A5077973612- interferon and immune responses
- Bacteriophages and microbial interactions
- Immune Response and Inflammation
- Antibiotic Resistance in Bacteria
- Animal Disease Management and Epidemiology
- Reproductive Physiology in Livestock
- Biochemical and Structural Characterization
- Effects of Environmental Stressors on Livestock
- Antimicrobial Resistance in Staphylococcus
- Probiotics and Fermented Foods
- Phytochemicals and Antioxidant Activities
- Free Radicals and Antioxidants
- Ion Transport and Channel Regulation
- Inflammasome and immune disorders
- Milk Quality and Mastitis in Dairy Cows
- Genetic and phenotypic traits in livestock
- RNA regulation and disease
- Animal Genetics and Reproduction
- Microbial infections and disease research
- Viral Infections and Vectors
- Essential Oils and Antimicrobial Activity
- Antimicrobial Peptides and Activities
- thermodynamics and calorimetric analyses
- Microbial Metabolism and Applications
- Heat shock proteins research
The Pirbright Institute
2024
National Dairy Research Institute
2015-2024
Higher School of Applied Sciences
2020
International Higher School of Medicine
2020
Royal Veterinary College
2018
Congenital anomalies of kidney and urinary tract (CAKUT), including vesico-ureteric reflux (VUR), are major causes ESRD in childhood. Herein is reported evidence for a locus on 13q33q34 associated with CAKUT. Deletion mapping chromosome 13q was performed four children CAKUT using 31 microsatellite markers peripheral blood genomic DNA that obtained from the patients their parents. mRNA expression positional candidate genes compared sequences electronic databases silico also studied adult...
For early diagnosis of infections, a rapid, sensitive and precise method to identify microbial DNA coming from veterinary clinical samples, such as milk cows affected with mastitis, is fascinating. We developed an amplification-free visual assay for rapid detection specific Escherichia coli, particularly uid A gene encoding beta-glucuronidase, isolated in based on multiple gold nanoparticles (AuNPs) captured magnetic microbead surface, leading plasmonic signal enhancement, thereby improving...
ALRs (AIM2-like Receptors) are germline encoded PRRs that belong to PYHIN gene family of cytokines, which having signature N-terminal PYD (Pyrin, PAAD or DAPIN) domain and C-terminal HIN-200 (hematopoietic, interferon-inducible nuclear protein with 200 amino acid repeat) joined by a linker region. The positively charged senses binds negatively phosphate groups single-stranded DNA (ssDNA) double-stranded (dsDNA) purely through electrostatic attractions. On the other hand, interacts...
Polyinosinic:polycytidylic acid or poly(I:C) is a double stranded RNA analog that known for stimulating sensing pathways in variety of cell culture and animal models. Activation nucleic lead to the up-regulation Interferon-β which ultimately creates an antiviral state host. The involvement intracellular DNA sensors mediated immune response has not been extensively explored. Poly(I:C) stimulates addition buffalo fibroblasts. Genomic leak into cytosol due likely stimulate pathways. mRNA...
Abstract Inflammasomes are multiprotein caspase‐activating complexes that enhance the maturation and release of proinflammatory cytokines (IL‐1β IL‐18) in response to invading pathogen and/or host‐derived cellular stress. These assembled by sensory proteins (viz NLRC4, NLRP1, NLRP3, AIM‐2), adaptor protein (ASC), effector molecule procaspase‐1. In NLRP3‐mediated inflammasome activation, ASC acts as a mediator between NLRP3 procaspase‐1 for transmission signals. A series homotypic...
In eukaryotes, the heat shock factors (HSFs) are recognized as master regulator of response. this respect, genes encoding seem to be important for adaptation thermal stress in organisms. Despite this, only few mammalian HSFs has been characterized. study, four major factor viz. HSF-1, 2, 4, and 5 were studied. The main objective present study was characterize cDNA using conserved gene specific primers investigate expression status these buffalo HSF genes. Our RT-PCR analysis uncovered two...
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Nucleotide-binding and oligomerization domain-containing protein 2 (NOD2) recognizes the muramyl dipeptide activates NF-κB signaling cascade following its interaction with receptor-interacting (RIP2) via caspase recruitment domains (CARDs). The NOD2-RIP2 is not understood well due to inadequate structural information. Using comparative modeling multimicrosecond timescale molecular dynamics simulations, we have demonstrated association of NOD2-CARDs (CARDa-CARDb) their RIP2CARD. Our results...
The Anti-Müllerian Hormone (AMH) is a member of the transforming growth factor beta (TGF-β) superfamily, playing significant role in cell proliferation, differentiation and apoptosis. In females, AMH secreted throughout their reproductive life span from ovaries, whereas males it by gonadal cells at very early stage testicular development. promising marker ovarian reserve women can be used to measure female lifespan. present study, we cloned sequenced GC rich gene Indian riverine buffalo (...
Estrus detection in buffaloes primarily relies on behavioral and physiological signs. Especially during summer, these signs are less prominent to recognize. Thus, estrus is a pronounced challenge within the realm of buffalo husbandry, particularly summer. Therefore, simple accurate method required for farmers. The observation fern-like salivary crystallization patterns one such detect buffaloes, bactrian camels, beagle bitches, cows. However, exact mechanism formation typical not known. We...
Abstract Type III interferon (IFN), also known as IFN-λ, is an innate antiviral protein. We retrieved the sequences of IFN-λ and their receptors from 42 tetrapod species conducted a computational evolutionary analysis to understand diversity these genes. The copy number variation (CNV) was determined through qPCR in Indian cattle buffalo. feature intron-containing type IFN Some reptiles placental mammals have 2 loci, while marsupials, monotremes, birds single locus. amphibians exhibit...
Whereas the intranasally delivered influenza vaccines used in children affect transmission of virus community as well reducing illness, inactivated administered by intramuscular injection do not prevent and have a variable, sometimes low rate vaccine effectiveness. Although mucosally potential to induce more protective immune response at site viral infection, quantitating such responses large scale clinical trials developing correlates protection is challenging. Here we show that using...