- Odor and Emission Control Technologies
- Microbial bioremediation and biosurfactants
- Microbial Community Ecology and Physiology
- Indoor Air Quality and Microbial Exposure
- Genomics and Phylogenetic Studies
- Wastewater Treatment and Nitrogen Removal
- Vehicle emissions and performance
- Water Treatment and Disinfection
- Urban Stormwater Management Solutions
- Environmental remediation with nanomaterials
- Methane Hydrates and Related Phenomena
- Chemical Reactions and Isotopes
- Microbial Fuel Cells and Bioremediation
- Chromium effects and bioremediation
- Toxic Organic Pollutants Impact
- Groundwater flow and contamination studies
- Microbial Applications in Construction Materials
- Biocrusts and Microbial Ecology
- Wind and Air Flow Studies
- Water Systems and Optimization
- Air Quality and Health Impacts
- Biofuel production and bioconversion
- Hydrocarbon exploration and reservoir analysis
- Plant Surface Properties and Treatments
- Slime Mold and Myxomycetes Research
Louisiana State University
2014-2025
The University of Texas at Austin
2005
American Society of Civil Engineers
2000
University of Notre Dame
1970-2000
Two recently reported bacterial strains that are able to reductively dehalogenate polychlorinated aliphatic alkanes, including 1,2,3-trichloropropane, 1,2-dichloropropane, 1,1,2,2-tetrachloroethane, 1,1,2-trichloroethane and 1,2-dichloroethane, were further characterized clarify their taxonomic position. The two strains, designated BL-DC-8 BL-DC-9T, mesophilic, non-spore-forming, non-motile, Gram-negative staining strictly anaerobic. Cells irregular cocci, 0.3–0.6 μm in diameter. resistant...
Hurricane Katrina, rated as a Category 4 hurricane on the Saffir−Simpson scale, made landfall U. S. Gulf Coast near New Orleans, Louisiana Monday, August 29, 2005. The storm brought heavy winds and rain to city, several levees intended protect Orleans from water of Lake Pontchartrain were breached. Consequently, up 80% city was flooded with reaching depths in excess three meters some locations. Research described this paper conducted provide an initial assessment contaminants present...
Summary Two strictly anaerobic bacterial strains were isolated from contaminated groundwater at a Superfund site located near Baton Rouge, LA, USA. These represent the first isolates reported to reductively dehalogenate 1,2,3‐trichloropropane. Allyl chloride (3‐chloro‐1‐propene), which is chemically unstable, was produced 1,2,3‐trichloropropane, and it hydrolysed abiotically allyl alcohol also reacted with sulfide‐ cysteine‐reducing agents in medium form various sulfides. Both dehalogenated...
Two strictly anaerobic bacterial strains, designated IP3-3(T) and SBP-1, were isolated from groundwater contaminated by chlorinated alkanes alkenes at a Superfund Site located near Baton Rouge, Louisiana (USA). Both strains reductively dehalogenate variety of polychlorinated aliphatic alkanes, including 1,2-dichloroethane, 1,2-dichloropropane, 1,1,2,2-tetrachloroethane, 1,1,2-trichloroethane 1,2,3-trichloropropane, when provided with hydrogen as the electron donor. To clarify their taxonomic...
A novel, Gram-negative bacterial strain, SUA2 T , isolated from groundwater, was characterized using a polyphasic approach. Cells are Gram-negative, non-spore-forming, straight to curved rods with single polar flagellum. Strain is oxidase- and catalase-positive able fix nitrogen. Poly- β -hydroxybutyrate storage granules produced. Dominant fatty acids when grown in R2A VM ethanol media for 72 h at 37 °C C 16 : 0 1 ω 7c, 17 cyclo, 10 3-OH, 18 12 15 . DNA G+C content 67.9 mol%. Phenotypic...
A strictly anaerobic, Gram-stain-negative, non-spore-forming bacterium designated NSZ-14T, isolated from contaminated groundwater in Louisiana (USA), was characterized using a polyphasic approach. Strain NSZ-14T reductively dehalogenated variety of polychlorinated aliphatic alkanes, producing ethene 1,2-dichloroethane, propene 1,2-dichloropropane, mixture cis- and trans-1,2-dichloroethene 1,1,2,2-tetrachloroethane, vinyl chloride 1,1,2-trichloroethane allyl (3-chloro-1-propene)...
Dehalogenimonas lykanthroporepellens is the type species of genus Dehalogenimonas, which belongs to a deeply branching lineage within phylum Chloroflexi. This strictly anaerobic, mesophilic, non spore-forming, Gram-negative staining bacterium was first isolated from chlorinated solvent contaminated groundwater at Superfund site located near Baton Rouge, Louisiana, USA. D. interest for genome sequencing two reasons: (a) an unusual ability couple growth with reductive dechlorination...
Studies were conducted to evaluate use of granular activated carbon (GAC) as a passively operated load-equalization mechanism for biofilters treating gas streams with dynamically varying (intermittent) pollutant loading. In the initial stage research, abiotic fixed-bed sorption experiments and numerical modeling assess degree achieved by GAC columns air flows containing intermittent loading acetone toluene present single-component contaminants mixture. subsequent an integrated system...
Anaerobic degradation of cis-1,2-dichloroethene (cis-1,2-DCE) and 1,2-dichloroethane (1,2-DCA) was studied in microcosms derived from a laboratory-scale upflow treatment wetland system used to biodegrade chlorinated compounds present groundwater Superfund site. Dechlorination kinetics cis-1,2-DCE (0.94−1.57 d-1) 1,2-DCA (0.15−0.71 were rapid, proceeded completion with ethene or ethane as terminal dechlorination products. Hydrogen concentrations, measured simultaneously during dechlorination,...
Biological processes, most notably biofilters and biotrickling filters, are increasingly used to remove biodegrade a wide variety of volatile organic compounds (VOCs) present in gas streams emitted from industrial operations. In the research described herein, laboratory-scale biofilter was operated for period more than 180 days treat waste comprised four-component VOC mixture representative solvents off-gases by painting The biofilter, packed with cubed polyurethane foam media initially...
Two anaerobic bacterial strains, designated SHI-1(T) and SHI-2, were isolated from chlorinated solvent-contaminated groundwater. They found to be identical in phenotypic properties shared high (98.5-99.8 %) pairwise 16S rRNA gene sequence similarity. Multiple genes present the isolates as well Pelosinus fermentans DSM 17108(T) Sporotalea propionica 13327(T). Strains SHI-2 could differentiated their closest phylogenetic relatives, P. S. 13327(T), on basis of properties. The Gram-negative,...
Abstract The contaminant concentrations over which type strains of the species Dehalogenimonas alkenigignens and lykanthroporepellens were able to reductively dechlorinate 1,2-dichloroethane (1,2-DCA), 1,2-dichloropropane (1,2-DCP), 1,1,2-trichloroethane (1,1,2-TCA) evaluated. Although initially isolated from an environment with much lower halogenated solvent , D. IP3-3 T was found dehalogenate chlorinated alkanes at comparable BL-DC-9 . Both dechlorinated 1,2-DCA, 1,2-DCP, 1,1,2-TCA present...
Although biological treatment has been applied successfully to treat waste gases using conventional organic packing media such as compost, there is currently a need for further research on ways optimize the medium selected. Use of superior filter-bed could overcome or minimize many problems encountered in biofilter operation (e.g., clogging, difficulty control moisture content, and nutrient limitation). In described herein, polyurethane foam was manufactured characterized determine its...
Bacterial concentration and diversity was assessed in a moderately acidic (pH 5.1) anaerobic groundwater contaminated by chlorosolvent-containing DNAPL at Superfund site located near Baton Rouge, Louisiana. Groundwater analysis revealed total aqueous-phase chlorosolvent exceeding 1000 mg L(-1), including chloroethanes, vinyl chloride, 1,2-dichloropropane, hexachloro-1,3-butadiene as the primary contaminants. Direct counting of stained cells more than 3 x 10(7) mL(-1) groundwater, with 58%...
Two novel facultatively anaerobic bacterial strains, BL-34 T and BL-35, isolated from groundwater contaminated by a mixture of chlorosolvents were characterized using polyphasic approach. The two strains exhibited essentially identical taxonomic features except for vitamin B 12 requirement strain BL-35 optimal growth. Phylogenetically, the isolates affiliated with members family Propionibacteriaceae placed in phylogenetic branch adjacent to, but distinct from, those genera Propionimicrobium...
Although anaerobic bioremediation of chlorinated organic contaminants in the environment often requires exogenous supply hydrogen as an electron donor, little is known about ability hydrogen-producing bacteria to grow presence solvents. In this study, 18 Clostridium strains including nine uncharacterized isolates originating from solvent contaminated groundwater were tested determine their fermentatively produce three common aliphatic contaminants: 1,2-dichloroethane (DCA),...
Members of the haloalkane dechlorinating genus Dehalogenimonas are distantly related to "Dehalococcoides" but share high homology in some variable regions their 16S rRNA gene sequences. In this study, primers and PCR protocols intended uniquely target Dehalococcoides were reevaluated, developed tested. Use genus-specific revealed presence both bacterial groups groundwater at a Louisiana Superfund site.
A polyurethane foam medium with characteristics described in Part I of this paper was tested a toluene degrading biofilter to demonstrate its ability support an active biofilm and study feasibility novel nutrient addition biomass wasting strategy. laboratory-scale fed model waste stream containing for more than 300 days using empty bed residence times ranging from 1 4 min concentrations 50 200 parts per million by volume. Results reported herein that high porosity, suitable pore size, low...