A5073982985- Microbial Metabolic Engineering and Bioproduction
- Biochemical and biochemical processes
- Lignin and Wood Chemistry
- Biofuel production and bioconversion
- Microplastics and Plastic Pollution
- Bacterial Genetics and Biotechnology
- Microbial Community Ecology and Physiology
- biodegradable polymer synthesis and properties
- Marine Bivalve and Aquaculture Studies
- Polymer composites and self-healing
- Enzyme-mediated dye degradation
- Genomics and Phylogenetic Studies
- Microbial metabolism and enzyme function
- RNA and protein synthesis mechanisms
- Plant tissue culture and regeneration
- Biofield Effects and Biophysics
- Tardigrade Biology and Ecology
- Fermentation and Sensory Analysis
- Microbial bioremediation and biosurfactants
- Graphene and Nanomaterials Applications
- Microbial Natural Products and Biosynthesis
- Plant-Microbe Interactions and Immunity
- Enzyme Catalysis and Immobilization
- Fatty Acid Research and Health
- Microbial Inactivation Methods
Oak Ridge National Laboratory
2021-2025
Scripps Institution of Oceanography
2017-2024
University of California, San Diego
2017-2021
Scripps Research Institute
2020
Abstract The accumulation of microplastics in various ecosystems has now been well documented and recent evidence suggests detrimental effects on biological processes due to this pollution. Accumulation the natural environment is ultimately chemical nature widely used petroleum-based plastic polymers, which typically are inaccessible processing. One way mitigate crisis adoption plastics that biodegrade if released into environments. In work, we generated microplastic particles from a...
The transition to renewable plastics will require the development of substitutes with existing industrial standards and manufacturing processes. Polyurethanes (PU), versatile plastics, are traditionally dominated by aromatic diisocyanates, which challenging derive from sources. However, for higher biocontent, it is crucial utilize aliphatic can be sourced plant or algae waste streams. Historically, PU foams relied on diisocyanates essential hard segments, resulting in desired physical...
Methane utilization by methanotrophic bacteria is an attractive application for biotechnological conversion of natural or biogas into high-added-value products. Haloalcaliphilic belonging to the genus Methylomicrobium are among most promising strains methane-based biotechnology, providing easy and inexpensive cultivation, rapid growth, availability established genetic tools. A number methane bioconversions using microbial cultures have been discussed, including derivation biodiesel, alkanes,...
ABSTRACT Lignin contains a variety of interunit linkages, leading to range potential decomposition products that can be used as carbon and energy sources by microbes. β-O-4 linkages are the most common in native lignin, associated catabolic pathways have been well characterized. However, fate mono-aromatic intermediates result from dimer cleavage has not fully elucidated. Here, we experimental evolution identify mutant strains Novosphingobium aromaticivorans with improved catabolism model...
Aromatic compounds are an important source of commodity chemicals traditionally produced from fossil fuels. Aromatics derived plant lignin can potentially be converted into through depolymerization followed by microbial funneling monomers and low molecular weight oligomers. This study investigates the catabolism β-5 linked aromatic dimer dehydrodiconiferyl alcohol (DC-A) bacterium
Mobilizable plasmids are extra-chromosomal, circular DNA that have contributed to the rapid evolution of bacterial genomes and been used in environmental, biotechnological, medicinal applications. Degradative with genetic capabilities degrade organic contaminants, such as polycyclic aromatic hydrocarbons (PAHs), potential be useful for more environmentally friendly cost-effective remediation technologies compared existing physical methods. Genetic bioaugmentation, addition catabolic genes...
A bacterial isolate, B1D3AT, was isolated from river sediment collected the Hiwassee River near Calhoun, TN, by enrichment culturing with a model 5-5' lignin dimer, dehydrodivanillate, as its sole carbon source. B1D3AT also shown to utilize several lignin-derived monomers and dimers sources in variety of minimal media. Cells were Gram-stain-negative, aerobic, motile, rod-shaped formed yellow/cream-coloured colonies on rich agar. Optimal growth occurred at 30 °C, pH 7-8, absence NaCl. The...
Various marine gamma-proteobacteria produce omega-3 polyunsaturated fatty acids, such as eicosapentaenoic acid (20:5, EPA) and docosahexaenoic (22:6, DHA), which are incorporated into membrane phospholipids. Five genes, designated pfaABCDE, encode the polyketide/fatty synthase necessary for production of these long-chain acids. In addition to de novo biosynthesis EPA DHA, "Pfa synthase" is also involved with a hydrocarbon product (31:9, PUHC) in conjunction oleABCD pathway. this work, we...
The production of long-chain omega-3 polyunsaturated fatty acids (PUFA) by marine Gammaproteobacteria , particularly those from deep-sea environments, has been known for decades. These unique are produced a polyketide-type mechanism and subsequently incorporated into the phospholipid membrane. While much research focused on biosynthesis genes, their products, phylogenetic distribution these gene clusters, no prior studies have detailed genetic regulation this pathway. This study describes...
The biosynthesis and incorporation of polyunsaturated fatty acids into phospholipid membranes are unique features certain marine Gammaproteobacteria inhabiting high-pressure and/or low-temperature environments. In these bacteria, monounsaturated saturated produced via the classical dissociated type II acid synthase mechanism, while omega-3 such as eicosapentaenoic (EPA; 20:5n-3) docosahexaenoic (DHA; 22:6n-3) by a hybrid polyketide/fatty synthase-encoded pfa genes-also referred to secondary...
Abstract Lignin contains a variety of interunit linkages, which leads to range potential decomposition products that can be used as carbon sources by microbes. β-O-4 linkages are the most common in native lignin and associated catabolic pathways have been well characterized. However, fate mono-aromatic intermediates result from dimer cleavage has not fully elucidated. Here, we experimental evolution identify mutant strains Novosphingobium aromaticivorans with improved catabolism model...
Pressure is a key environmental parameter that influences the activity and distribution of microbial life on our planet. Despite its role there still not definitive list essential genes for adaptation to under increasing pressure. In this study we used previously characterized Escherichia coli strain (AN62) evolved grow at pressure (60 MPa) non-permissive parental performed comparative genomics in order identify genome-level adaptations might allowed observed pressure-adapted phenotype. We...
ABSTRACT A randomly barcoded transposon insertion sequencing (RB-TnSeq) library of Novosphingobium aromaticivorans DSM12444 was grown in media containing either glucose or the β-5-linked aromatic dimer dehydrodiconiferyl alcohol (DC-A) as sole carbon source. The cultures were to saturation and then sequenced, yielding barcode abundance data sets presented here.
Kurzfassung Eine überlegene Logistik ist für produzierende Unternehmen ein entscheidender Wettbewerbsvorteil. hohe Liefertreue logistisches Ziel und oftmals auch entscheidendes Kriterium bei der Lieferantenauswahl. Um eine gegenüber dem Kunden zu erreichen, Termintreue in Fertigung maßgebend. Diese kann mit einer gut konfigurierten implementierten Fertigungssteuerung erreicht werden. Dieser Beitrag zeigt am Beispiel Trumpf Grüsch AG, wie die deutlich verbessert werden kann.
Abstract A characteristic among many marine Gammaproteobacteria is the biosynthesis and incorporation of omega-3 polyunsaturated fatty acids into membrane phospholipids. Biosynthesis eicosapentaenoic (EPA) and/or docosahexaenoic (DHA) accomplished using a polyketide/fatty acid synthase mechanism encoded by set five genes pfaABCDE. This unique synthesis (FAS) pathway co-exists with canonical Type II dissociated pathway, which responsible for saturated, monounsaturated, hydroxylated used in...