This study reports on the total phenolic content and antioxidant activity as well compounds that are present in Calothamnus spp. (Red Bell), Agonis flexuosa (Coastal Peppermint), Corymbia calophylla (Marri) Eucalyptus marginata (Jarrah) honeys from Western Australia. The honey's (TPC) was determined using a modified Folin-Ciocalteu assay, while their FRAP DPPH assays. Phenolic constituents were identified High Performance Thin-Layer Chromatography (HTPLC)-derived database, quantified HPTLC....

The complex chemical composition of honey presents significant challenges for its analysis with variations influenced by factors such as botanical source, geographical location, bee species, harvest time, and storage conditions. This study aimed to employ high-performance thin-layer chromatography (HPTLC) fingerprinting, coupled multivariate data analysis, characterise the profiles Australian stingless samples from two distinct Tetragonula carbonaria hockingsi. Using a mobile phase composed...

The antibacterial activity of honeys derived from the endemic flora southwest corner Western Australia, including trees Jarrah (Eucalyptus marginata) and Marri (Corymbia calophylla), remains largely unexplored. Investigation these showed minimum inhibitory concentrations (MICs) 6.7-28.0% (w/v) against Gram positive negative bacteria. Honey solutions enhanced after hydrogen peroxide was allowed to accumulate prior testing, with a mean MIC accumulation 14.3% compared 17.4% before accumulation....

Honey adulteration, where a range of sugar syrups is used to increase bulk volume, common problem that has significant negative impacts on the honey industry, both economically and from consumer confidence perspective. This paper investigates High-Performance Thin Layer Chromatography (HPTLC) for authentication detection adulterants in honey. The composition various Australian honeys (Manuka, Jarrah, Marri, Karri, Peppermint White Gum) was first determined illustrate variance depending...

Article Investigation of In-Vitro Antioxidant, Antimicrobial, Thrombolytic and In-Vivo Anti-Inflammatory Antidepressant Potential Holigarna Longifolia Leaf Extract Md Mizan 1,2,*, Shawon Miah 1 Khairul Islam Department Pharmacy, Dhaka International University, Satarkul, Badda, 1212, Bangladesh 2 Pharmacy Discipline, School Life Science, Khulna 9208, * Correspondence: hellotomizan@gmail.com; Tel.: +88-1746469198 Received: 11 January 2025; Revised: 9 February Accepted: 18 Published: 20 2025...

The use of High-Performance Thin-Layer Chromatography (HPTLC) coupled with the DPPH* (2,2-diphenyl-1-picrylhydrazyl) as a derivatisation reagent is novel approach to analysis antioxidant activity honeys. method facilitates visualisation individual constituents that contribute overall honey, even if they are not yet chemically identified, and allows for quantification their gallic acid equivalents. supports more in-depth study honey it comparative fingerprints honeys different floral origin...

This study reports on the analysis of eleven Jarrah (Eucalyptus marginata) honeys, which nearly half (n = 5) were re-classified as Blackbutt (E. patens) honey grounds predominant flower pollen identified by melissopalynology. Based a comprehensive honeys' physico- and phytochemical characteristics antioxidant activity data, taking into account pH, electrical conductivity, refractive index Brix values well moisture content, individual fructose glucose content derived to ratio alongside total...

Honeys produced by Apis mellifera from different flowering plants can vary widely in their characteristics. Some floral sources yield honeys with especially high antibacterial and/or antioxidant activity, which may be candidates for further evaluation as therapeutic agents. This study investigated 115 harvested three Western Australian coastal bioregions: the Swan plain, Geraldton sandplains and Dampierland. were derived a range of sources, including Agonis, Banksia, Calothamnus,...

Abstract A novel, simple and reliable high-performance thin-layer chromatography (HPTLC) method was developed validated for the quantification of trehalulose in stingless bee honey. The chromatographic separation performed using silica gel 60 F 254 HPTLC plates 1-butanol‒2-propanol‒aqueous boric acid solution (5 mg/mL) (30:50:10, V/V ) as mobile phase. retardation factor ( R found to be 0.045. showed linearity over concentration range 100–800 ng per band with a coefficient correlation...

This study reports on the physicochemical and antioxidant properties of propolis samples from various regions across Western Australia identifies some phenolic constituents using high-performance thin-layer chromatography (HPTLC). Total content (TPC) was determined a modified Folin-Ciocalteu assay, activity investigated with Ferric Reducing Antioxidant Power (FRAP) assay also visualised semi-quantified by HPTLC-DPPH analysis. TPC values ranged 9.26 to 59.3 mg gallic acid equivalent/g raw...

In this paper, we describe a novel approach to the development of reference standard for quality control complex natural products, which will assist in assessment their authenticity and purity. The proposed method provides template selection samples, can be pooled obtain standard. A shortfall such an is, however, that sample is static nature therefore unable capture difference processing conditions or variations triggered by geographical climatic impacts over time. To address this, paper...

Jarrah ( Eucalyptus marginata ) is a dominant forest tree endemic to the southwest of Western Australia. Its honey appreciated for its highly desirable taste, golden colour, slow crystallisation, and high levels bioactivity, which have placed in premium product range. However, whilst customers are willing pay price this natural product, there currently no standard method authentication. As naturally sourced from flower nectar, novel route authentication identify nectar signature within...

In this study, a high-performance thin-layer chromatography (HPTLC)-based method was developed for the quantification of five water-soluble vitamins, B2, B3, B6, B12, and C, using ethanol–water (70:30, v/v) as mobile phase. The validated according to International Conference on Harmonisation (ICH) guidelines limits detection/limits were determined 5.27/15.97 ng (Vitamin B2), 13.03/39.47 B3), 59.61/180.64 B6), 14.17/42.93 B12), 119.27/361.42 C) per band, respectively. usefulness quality...

Despite its cultural and nutritional importance for local Aboriginal people, the unusual insect honey produced by Western Australian honeypot ant (Camponotus inflatus) has to date been rarely investigated. This study reports on honey's physicochemical properties, total phenolic, major sugars 5-hydroxymethylfurfural contents, antioxidant activities. The color value is 467.63 mAU/63.39 mm Pfund, it a pH of 3.85, electric conductivity 449.71 µSiemens/cm. Its Brix 67.00, corresponding 33%...

High-Performance Thin-Layer Chromatography (HPTLC) was used in a chemometric investigation of the derived sugar and organic extract profiles two different honeys (Manuka Jarrah) with adulterants. Each honey adulterated one six syrups (rice, corn, golden, treacle, glucose maple syrups) five concentrations (10%, 20%, 30%, 40%, 50% w/w). The analysis based on combined profiles’ datasets. To obtain respective profiles, amount fructose, glucose, maltose, sucrose present quantified for profile,...

Honeys are commonly subjected to a series of post-harvest processing steps, such as filtration and/or radiation treatment and heating various temperatures, which might affect their physicochemical properties bioactivity levels. Therefore, there is need for robust quality control assessments after honey storage ensure that the exposure higher example, does not compromise honey's chemical composition antioxidant activity. This paper describes comprehensive short-term (48 h) long-term (5...

A simple, cost-effective, and efficient novel high-performance thin-layer chromatography (HPTLC) tool was developed validated in accordance with International Conference on Harmonisation (ICH) guidelines for the detection quantification of four fat-soluble vitamins, D2, D3, E, K1, using chloroform: cyclohexane (55:45, v/v) as mobile phase. The limits were found to be 30.86 93.52 ng/band vitamin 19.44 58.92 14.17 42.95 0.86 2.61 which similar or lower than those reported previous methods....

The aim of this study was to examine a collection 79 honeys derived from plants endemic several Western Australian unique bioregions for bioactivity and physicochemical characteristics. For analyses, total phenolic content, high performance thin layer chromatography (HPTLC) fingerprints, pH, Brix, colour hydrogen peroxide generation were examined. Brix (82.6±1.3) pH (4.34±0.24) values within expected ranges, whereas levels determined using an o-dianisidine/horseradish peroxidase assay...