A5038611881- Freshwater macroinvertebrate diversity and ecology
- Environmental DNA in Biodiversity Studies
- Aquatic Invertebrate Ecology and Behavior
- Species Distribution and Climate Change
- Fish Ecology and Management Studies
- Genetic diversity and population structure
- Fossil Insects in Amber
- Lepidoptera: Biology and Taxonomy
- Plant and animal studies
- Invertebrate Taxonomy and Ecology
- Hemiptera Insect Studies
- Insect and Arachnid Ecology and Behavior
- Evolution and Genetic Dynamics
- Diptera species taxonomy and behavior
- Marine Ecology and Invasive Species
- Amphibian and Reptile Biology
- Microbial Community Ecology and Physiology
- Identification and Quantification in Food
- Genomics and Phylogenetic Studies
- Animal Behavior and Reproduction
- Bat Biology and Ecology Studies
- Forest Insect Ecology and Management
- Marine Biology and Ecology Research
- Fish biology, ecology, and behavior
- Silk-based biomaterials and applications
Shinshu University
2015-2024
Ōtani University
2015-2024
Nagano University
2011-2024
Shinshu University Hospital
2006-2023
Shimadzu (China)
2018
Kansai Medical University
2014
Hokkaido University
2013
Japan Fisheries Research and Education Agency
2009-2011
Matsumoto University
2007
Kanagawa Environmental Research Center
2007
Abstract Japan is considered a global hot spot of biodiversity. With regard to species diversity, insects are no exception. To date, more than 32,000 insect have been identified in Japan, while around 100,000 estimated inhabit this country. In paper, we outline background factors having contributed diversification Japanese insects. Of course, the high degree diversity result many complex factors. addition humid Asian monsoon climate and extensive latitudinal gradient habitats, extremely...
Insect wing is a key evolutionary innovation for insect radiation, but its origins and intermediate forms are absent from the fossil record. To understand ancestral state of wing, expression three regulatory genes in development, wingless (wg), vestigial (vg), apterous (ap) was studied two basal insects, mayfly bristletail. These insects develop dorsal limb branches, tracheal gill stylus, respectively, that have been considered candidates origin. Here we show wg vg expressed primordia...
The use of environmental DNA (eDNA) has recently been employed to evaluate the distribution various aquatic macroorganisms. Although this technique applied a broad range taxa, from vertebrates invertebrates, its application is limited for insects such as heteropterans . Nepa hoffmanni (Heteroptera: Nepidae) small (approx. 23 mm) heteropteran that inhabits wetlands, can be difficult capture and endangered in Japan. molecular tool eDNA was used species N. comparison determined using...
Insect silk is a versatile biomaterial. Lepidoptera and Trichoptera display some of the most diverse uses silk, with varying strength, adhesive qualities, elastic properties. Silk fibroin genes are long (>20 Kbp), many repetitive motifs that make them challenging to sequence. Most research thus far has focused on conserved N- C-terminal regions because full comparison across taxa not been possible. Using PacBio Sequel II system SMRT sequencing, we generated high fidelity (HiFi) long-read...
Abstract Aim Pelophylax frogs in East Asia provide an opportunity to explore the impact of glacial cycling on demographic and genetic dynamics, because it has been suggested that they experienced distribution shifts subsequent mt DNA introgression from plancyi nigromaculatus association with climatic oscillations. However, their evolutionary history, including pattern introgression, is incompletely understood. We used phylogenetic analyses based multiple markers address palaeodistribution...
A new mayfly species, Bleptus michinokuensis sp. nov. (Ephemeroptera: Heptageniidae) is described on the basis of specimens male and female adults mature nymphs collected at a seepage zone small freshwater branch ‘Tachiya-zawa-gawa’ River located amongst northern foothills Mt. Gassan (Shonai-machi Town, Yamagata Prefecture, Japan). This species characterized by its clear fore hind wings. That is, they neither exhibit distinct black band wings, nor characteristic darkened margins along edges...
In East Asia, the mayfly genus Ephoron, a member of Polymitarcyidae, has been classified into three species: Ephoron eophilum, limnobium, and shigae. Using nuclear histone 3 internal transcribed spacer 1 mitochondrial 16S rRNA cytochrome c oxidase subunit I genes, we investigated phylogenetic relationships these mayflies. limnobium formed polyphyletic within clade Japanese E. shigae shared identical sequences with shigae, suggesting that is synonym Monophyly was supported in Korean +...
The Japanese Islands have a high degree of biodiversity, with almost the entire area being designated as 'global hotspot biodiversity'. There is no doubt that many complex factors contributed to this, particularly history geological formation Islands. As such, we focused on genetic structure and evolutionary dipteromimid mayflies. In this study, 392 specimens from 158 localities were collected analysed genetically. Phylogenetic analyses performed using mitochondrial DNA 16S rRNA COI regions...
We focused on the genetic structure and evolutionary divergence of a typical habitat generalist mayfly, Isonychia japonica, inhabiting rivers widely distributed across eastern Asia. In Japanese Archipelago, they are wide range environments, including up- downstream areas in 1 river basin. analyzed DNA sequences this mayfly from both mitochondrial (cytochrome oxidase c subunit I [COI] 16S ribosomal RNA) nuclear genomes (Histone H3 ITS2) specimens populations Asian region, with emphasis...
Underwater optical wireless communication has been merely a theory for long time because light sources are too weak to use them as emitters communications. In the past decade, however, underwater communications have used laser diodes or emitting with visible in high brightness low power consumption. Recently, they become practical. As described this paper, recent trends of study, practical modems and prospective uses presented first. Next, characteristics seawater various conditions...
Elucidation of the diversification process organisms is one important tasks biology. From viewpoint species diversity, insects are most successful group among diverse on earth and evolutionary adaptation factors driving this pattern. Evolutionary in insects. One representative examples environmental shortening loss wings subalpine alpine zones. In study, we focused Japanese scorpionfly, Panorpodes paradoxus. species, individuals that inhabit mountainous regions zones have long (the "general...
Abstract Diving beetles play an important role in fishless freshwater communities. The genus Cybister is included the Japanese Red Data List owing to its diminished population size. phylogenetic relationships and genetic structures of chinensis brevis, whose populations are declining, tripunctatus lateralis, distribution increasing, poorly understood must be addressed future conservation efforts. In this study, we investigated flight behaviour phylogeography three species. Cybistyer...
Abstract Long‐term biodiversity monitoring is necessary for conservation and management. In such circumstances, environmental DNA (eDNA) surveys can enable easy effective biomonitoring of aquatic insects. However, previous studies insects based on the mtDNA COI region have revealed incomplete taxonomic coverage, frequent amplification nontarget taxa (e.g., algae diatoms). Additionally, it has been indicated that there are few reference sequences registered in databases metabarcoding analysis...
Evaluating the relative connectivity of populations within and among streams in a riverine landscape is crucial to developing better understanding stream ecology. Molecular markers provide reasonable approach assess evaluate inherent properties populations, such as genetic diversity. Many previous investigators differentiation species at regional level have focused on habitat specialists, but generalists, with their continuous broad distribution across wide variety habitats, not been focus...
Phylogeographic studies based on molecular information have been attracting attention because they come to play a significant role in the elucidation of population structure and phylogenetic relationships species. Furthermore, tools helped reveal evidence for cryptic biodiversity, including many cases discovery new We focused phylogeography stenopsychid caddisflies. The family Stenopsychidae has distribution area mainly within Southern Hemisphere, only Stenopsyche caddisflies spread into...
In the present study, we add genetic data of mayfly Cloeon dipterum collected from Japanese Islands to established molecular phylogenetic knowledge in mitochondrial COI gene mayflies. is a typical cosmopolitan species that includes six intraspecific haplotype groups. The analysis revealed haplotypes C. constitute seventh group together with Korea. This East Asian forms sister previously known European and North American groups (i.e., clade CT1 CT3). further occurrence two described (C....
Carnitine palmitoyltransferase II (CPT II) deficiency is an inherited disorder involving β‐oxidation of long‐chain fatty acids. CPT a wide‐spectrum that includes lethal neonatal form, infantile and adult‐onset form. However, the ethnic characteristics relationship between genotype clinical manifestation are not well understood. We investigated three non‐consanguineous Japanese patients with examined cell lines from 4 unrelated 50 healthy donors. The 2 gene was typed by direct DNA sequencing...
In the geographically parthenogenetic mayfly, Ephoron shigae, egg maturation and counts of chromosome number unfertilized, eggs were studied, in comparison with fertilized from a bisexual population. The primary oocyte becomes mature through two successive divisions. first division (meiotic division) takes place to produce secondary polar body. second soon occurs oocyte, which nucleus is divided into (female pronucleus) body nuclei. body, some cases, was successively bodies; other instances,...