Remote polar and deepwater fish faunas are under pressure from ongoing climate change increasing fishing effort. However, these communities difficult to monitor for logistic financial reasons. Currently, monitoring of marine fishes largely relies on invasive techniques such as bottom trawling, official reporting global catches, which can be unreliable. Thus, there is need alternative non-invasive qualitative quantitative oceanic surveys. Here we report environmental DNA (eDNA) metabarcoding...

The European weather loach (Misgurnus fossilis) represents one of many freshwater fishes in decline. Efficient monitoring is essential if conservation efforts are to be successful, but due the species' cryptic biology, traditional methods currently use inefficient, time consuming and likely prone non-detection error. Here, we investigate usefulness environmental DNA (eDNA) as an alternative or supplementary method for surveying Danish population, which presumed consist primarily a single...

Summary Aqueous environmental DNA ( eDNA ) is an emerging efficient non‐invasive tool for species inventory studies. To maximize performance of downstream quantitative PCR (qPCR) and next‐generation sequencing NGS applications, quality quantity the starting material crucial, calling optimized capture, storage extraction techniques . Previous comparative studies capture/storage have tested precipitation ‘open’ filters. However, practical ‘enclosed’ filters which reduce unnecessary handling...

Terrestrial arthropods comprise the most species-rich communities on Earth, and grassland flowers provide resources for hundreds of thousands arthropod species. Diverse ecosystems worldwide are threatened by various types environmental change, which has led to decline in diversity. At same time, monitoring diversity is time-consuming strictly dependent declining taxonomic expertise. Environmental DNA (eDNA) metabarcoding complex samples demonstrated that information species compositions can...

Abstract Conservation and management of marine biodiversity depends on biomonitoring habitats, but current approaches are resource‐intensive require different for organisms. Environmental DNA (eDNA) extracted from water samples is an efficient versatile approach to detecting aquatic animals. In the ocean, eDNA composition reflects local fauna at fine spatial scales, little known about effectiveness eDNA‐based monitoring communities larger scales. We investigated potential characterize...

Abstract Temporal variation in eDNA signals is increasingly explored for understanding community ecology aquatic habitats. Seasonal changes have been addressed using sampling, but very little known regarding short‐term temporal that spans hours to days. To address this, we filtered marine water samples from a single coastal site Denmark every hour 32 h. We used metabarcoding target both fish and broader eukaryote diversity evaluated this community. Results revealed species richness (15–27)...

Population genetic data can provide valuable information on the demography of a species. For rare and elusive marine megafauna, samples for generating are traditionally obtained from tissue biopsies, which be logistically difficult expensive to collect require invasive sampling techniques. Analysis environmental DNA (eDNA) offers an alternative, minimally approach important information. Although eDNA approaches have been studied extensively species detection biodiversity monitoring in...

Marine biodiversity is threatened by human activities. To understand the changes happening in aquatic ecosystems and to inform management, detailed, synoptic monitoring of across large spatial extents needed. Such challenging due time, cost, specialized skills that this typically requires. In an unprecedented study, we combined citizen science with eDNA metabarcoding map coastal fish at a national scale. We engaged 360 scientists collect filtered seawater samples from 100 sites Denmark over...

Abstract Environmental DNA (eDNA) provides a promising supplement to traditional sampling methods for population genetic inferences, but current studies have almost entirely focused on short mitochondrial markers. Here, we develop one and nuclear set of target capture probes the whale shark ( Rhincodon typus ) test them seawater samples collected in Qatar investigate potential eDNA‐based studies. The successfully retrieved ~235× (90× − 352× per base position) coverage mitogenome. Using minor...

Abstract While environmental DNA (eDNA) is becoming increasingly established in biodiversity monitoring of freshwater ecosystems, the use eDNA surveys marine environment still its infancy. Here, we two approaches: targeted quantitative PCR (qPCR) and whole‐genome enrichment capture followed by shotgun sequencing an effort to amplify killer whale from seawater samples. Samples were collected close proximity whales inshore offshore waters, varying sea conditions surface subsurface but none...

Abstract Environmental DNA (eDNA) metabarcoding is increasingly being implemented as a non‐invasive and efficient approach for biodiversity research monitoring across ecosystems. However, accurate detection of species with eDNA requires robust experimental designs analysis carries risk contamination at every step the fieldwork laboratory processes. Several studies focus on rigorous procedures processing sequencing data, but surprisingly, little investigates process background input in field....

Abstract Macroinvertebrate communities are crucial for biodiversity monitoring and assessment of ecological status in stream ecosystems. However, traditional approaches require intensive sampling rely on invasive morphological identifications that time‐consuming dependent taxonomic expertise. Importantly, is often only carried out once a year, namely during late winter–spring, where most indicator taxa have larval stages the streams. Hence, species with divergent phenology might not be...

Abstract Trophic rewilding is increasingly applied in restoration efforts, with the aim of reintroducing ecological functions provided by large‐bodied mammals and thereby promote self‐regulating, biodiverse ecosystems. However, empirical evidence for effects megafauna introductions on abundance richness other organisms such as plants invertebrates, mechanisms involved still need strengthening. In this study, we use environmental DNA (eDNA) metabarcoding dung from co‐existing feral cattle...

Abstract Insects and other terrestrial invertebrates are declining in species richness abundance. This includes the associated with herbivore dung, which have been negatively affected by grazing abandonment progressive loss of large herbivores since Late Pleistocene. Importantly, traditional monitoring these is time‐consuming requires considerable taxonomic expertise, becoming increasingly scarce. In this study, we investigated potential environmental DNA (eDNA) metabarcoding cow dung...

An assessment of vertical distribution, diel migration, taxonomic and functional diversity fishes was carried out at offshore platforms in The (Arabian–Iranian–Persian) Gulf. Video footage recorded the Al Shaheen oil field between 2007 2014 using a remotely operated vehicle (ROV). A total 12 822 individual fishes, from 83 groups were around platforms. All species identified are considered native to Gulf, although Cyclichthys orbicularis Lutjanus indicus for first time Qatari waters. Several...

Ocean circulation, geological history, geographic distance, and seascape heterogeneity play an important role in phylogeography of coral-dependent fishes. Here, we investigate potential genetic population structure within the yellowbar angelfish (Pomacanthus maculosus) across Northwestern Indian (NIO). We then discuss our results with respect to above abiotic features order understand contemporary distribution diversity species. To do so, restriction site-associated DNA sequencing (RAD-seq)...

Abstract Terrestrial invertebrates are highly important for the decomposition of dung from large mammals. Mammal has been present in many Earth's ecosystems millions years, enabling evolution a broad diversity dung‐associated that process various components dung. Today, herbivorous mammals increasingly introduced to with aim restoring ecological functions formerly provided by their extinct counterparts. However, we still know little about ecosystem and nutrient flows these rewilded...

Abstract The anthropogenic impact on the world's ecosystems is severe and need for non‐invasive, cost‐effective tools monitoring understanding those impacts are therefore urgent. Here, we combine two such methods in a comprehensive multi‐year study; camera trapping (CT) analysis of environmental DNA (eDNA), river marginal zones temperate, wetland Nature Park Denmark. CT was performed from 2015 to 2019 total 8778 trap days yielded 24,376 animal observations. observations covered 87 taxa,...

The Zebra Shark, in recent years known as Stegostoma fasciatum (Hermann, 1783), is well for its dramatic ontogenetic change of color pattern, from striped ("zebra") juveniles to spotted ("leopard") adults. Nevertheless, many aspects the species' biology, ecology, and morphology are still unknown or inadequately described, nomenclature contentious. This study introduces a hitherto undescribed morph Shark provides an updated diagnosis redescription species. Firstly, we establish that remains...

ABSTRACT Benthic macrofauna are important and widely used biological indicators of marine ecosystems as they have limited mobility therefore integrate the effects local environmental stressors over time. Recently, DNA (eDNA) analysis has provided a potentially more resource‐efficient approach for benthic biomonitoring than traditional morphology‐based methods. Several studies compared eDNA with monitoring, but few two approaches using exact same sediment cores. In addition, meiofauna pelagic...