Abstract Deposition of silver or compounds in proteins separated linear gradient polyacrylamide electrophoretic gels is influenced by protein concentration and location, concentration, extent washing after incubation ammoniacal hydroxide solution, development time a citric acid: formaldehyde solution. Our modification existing staining methods provided routine resolution polypeptides 1.0 to 1.5‐mm‐thick without high background surface with picogram detection sensitivity. Removing components,...

The effects of two novel quinolone derivatives, CP-67,804 and CP-115,953 (the 1-ethyl 1-cyclopropyl derivatives 6,8-difluoro-7-(4-hydroxyphenyl)-4-quinolone-3-carboxylic acid, respectively), on the enzymatic activities Drosophila melanogaster topoisomerase II were examined. Both drugs enhanced enzyme's pre- post-strand passage DNA cleavage activities. was nearly as potent an enhancer etoposide, while approximately 2 times more than this II-targeted antineoplastic drug. In contrast to which...

The extracellular amino acid composition of experimental wounds in rats during peak macrophage infiltration bears the imprint elevated arginase activity present wound fluid: L-arginine is found this space concentrations markedly lower, and L-ornithine higher, than those that are detectable plasma. No evidence, form L-citrulline or NO2- accumulation, can be at time for nitric oxide synthase (NOS) activity. Wound-derived macrophages, however, metabolize through both NOS culture. Given...

Journal Article High-Level Quinolone Resistance in Clinical Isolates of Campylobacter jejuni Get access John Segreti, Segreti Reprints or correspondence: Dr. Rush Medical College, 600 S. Paulina, Rm. 143 AcFac, Chicago, IL 60612. Search for other works by this author on: Oxford Academic PubMed Google Scholar Thomas D. Gootz, Gootz Larry J. Goodman, Goodman George W. Parkhurst, Parkhurst P. Quinn, Quinn Barbara A. Martin, Martin Gordon M. Trenholme The Infectious Diseases, Volume 165, Issue...

High-level resistance to quinolones has previously been shown occur in Campylobacter spp. both vitro and patients treated with quinolones. We have selected isolates that are resistant by plating cells from a susceptible C. jejuni strain, UA535, on medium containing nalidixic acid at 32 micrograms/ml. Fluctuation analysis indicated occurred mutation frequency of 5 x 10(-8) per cell plated. Unlike what is observed other gram-negative organisms, the acid-resistant mutants demonstrated...

A previous study (M.J. Robinson, B.A. Martin, T.D. Gootz, P.R. McGuirk, M. Moynihan, J.A. Sutcliffe, and N. Osheroff, J. Biol. Chem. 266:14585-14592, 1991) demonstrated that novel 6,8-difluoroquinolones were potent effectors of eukaryotic topoisomerase II. To determine the contribution C-8 fluorine to drug potency, we compared effects CP-115,955 [6-fluoro-7-(4-hydroxyphenyl)-1-cyclopropyl-4-quinolone-3-carboxylic acid] on enzymatic activities Drosophila melanogaster II with those CP-115,953...

COSGRIFF, T. M.; BISHOP, D. T.; HERSHGOLD, E. J.; SKOLNICK, M. H.; MARTIN, B. A.; BATY, CARLSON, K. S. Author Information

Activated murine peritoneal macrophage cytotoxicity against P815 tumor cells has been shown to be mediated by the reactive nitrogen intermediates (RNI) produced macrophages from L-arginine through nitric oxide (NO) synthase. Previous results this laboratory indicated that NO-dependent killing of fulfilled criteria for apoptotic death. Work others, in turn, demonstrated product bcl-2 gene confers protection various inducers apoptosis, including oxygen intermediates. Experiments were performed...

First posted June 15, 2017 For additional information, contact: Director, Western Fisheries Research Center U.S. Geological Survey 6505 NE 65th Street Seattle, Washington 98115 Populations of the once abundant Lost River (Deltistes luxatus) and shortnose suckers (Chasmistes brevirostris) Upper Klamath Basin, decreased so substantially throughout 20th century that they were listed under Endangered Species Act in 1988. Major landscape alterations, deterioration water quality, competition with...

Populations of the Lost River sucker Deltistes luxatus have declined so precipitously in Upper Klamath Basin Oregon and California that this fish was recently listed for federal protection as an endangered species. Although Lake is a major refuge species, lake occasionally experience mass mortalities during summer early fall. This field study implemented to determine if resulted from degraded water quality conditions associated with seasonal blooms phytoplankton, especially Aphanizomenon...

The clinical and cytogenetic findings of a female infant with multiple congenital anomalies trisomy for the short arm chromosome 4(46,XX,21p+) are described. abnormal was inherited from father who had balanced translocation between 4 21. Clinical features compared those one definite probable previously described case 4. It is suggested that syndrome associated +4p eventually may be identified.

Abstract Unsustainably high mortality within the first 2 years of life prevents endangered Lost River Suckers Deltistes luxatus in Upper Klamath Lake, Oregon, from recruiting to spawning populations. Massive blooms cyanobacterium Aphanizomenon flos‐aquae and their subsequent death decay lake (bloom‐crashes) are associated with pH, low percent oxygen saturation, total ammonia concentrations, spikes cyanotoxin microcystin. Poor water quality is considered most likely cause juvenile sucker...

ADVERTISEMENT RETURN TO ISSUEPREVArticleNEXTBiosynthesis of the 5,6-dimethylbenzimidazole moiety vitamin B12William L. Alworth, Harold Nordean Baker, Dwight Augustus Lee, and Barbara Anita MartinCite this: J. Am. Chem. Soc. 1969, 91, 20, 5662–5663Publication Date (Print):September 1, 1969Publication History Published online1 May 2002Published inissue 1 September 1969https://pubs.acs.org/doi/10.1021/ja01048a052https://doi.org/10.1021/ja01048a052research-articleACS PublicationsRequest reuse...