Activation of T cells by lectins or mAb directed at components the Ag-specific TCR results in hydrolysis phosphorylated derivatives phosphatidylinositol and an increase intracellular free calcium concentration (Cai). We report that cholera toxin, which activates adenylate cyclase ADP ribosylation a G protein, also reduces both inositol phosphate (IP) production rise Cai Con A-stimulated murine cells. find similar dose-dependent inhibitory effects can be induced each four other agents raise...

To combat pharmaceutical counterfeiting in low- and middle-income countries (LMIC), there is a need for improved low-cost, portable methods that monitor concentrations relevant to dosage forms physiological fluids.

Portable and inexpensive analytical tools are required to monitor pharmaceutical quality in technology limited settings including low- middle-income countries (LMICs). Whole cell yeast biosensors have the potential help meet this need. However, most of readouts for require expensive equipment or reagents. To overcome challenge, we designed a biosensor that produces unique scent as readout. This inducible biosensor, "scentsor", does not user administer additional reagents reporter development...

The NASA Langley Research Center Unitary Plan Wind Tunnel and the 20-Inch Mach 6 were used to test two osculating cones waverider models. Mach-4 Mach-6 shapes generated using interactive design tool WIPAR. WIPAR performance predictions are compared experimental results. Vapor screen results for model at on- number provide visual verification that shock is attached along entire leading edge, within limits of observation. pressure distributions aerodynamic coefficients show general agreement...

Complex adaptive systems are dynamically assembled characterized by multiple competing stakeholders, fluid requirements, emergent behavior, and susceptibility to external pressures that can cause change across the entire system. This paper shows how key principles of complex engineering support achieving net centric operations (NCO) for DoD. We discuss approaches on-demand data, an IT infrastructure strategy based on Web service semantic technologies, as well guidance initial observations...

The cooperation between the actin and microtubule (MT) cytoskeletons is important for cellular processes such as cell migration muscle development. However, a full understanding of how this occurs has yet to be sufficiently developed. MT plus-end tracking protein CLIP-170 been implicated in actin-MT coordination by associating with actin-binding signaling IQGAP1 promoting polymerization through binding formins. Thus far, interactions were assumed indirect. Here, we demonstrate using...

Net-Centric Solutions, as enabled through distributed service-oriented architectures, will have a significant effect on the way DoD acquires capabilities, thereby requiring new ways to address development and integration of complex enterprises. One approach, based commercial best practices, entails establishing an environment set processes for users developers work together in maturation capabilities they transform from innovation fielded capability. In this paper, we discuss approach that...

A computational model has been developed to predict and ultimately control the unsteady dynamics of aeroelastic systems. Through a general, curvilinear coordinate transformation, achieves exact coupling between fluid structure without compromising effects viscosity, separation, shocks, shock-boundary layer interaction. The method enables comprehensive approach analysis coupled-system dynamics: capturing weak nonlinearities in an eigensystem formulation, strong using full nonlinear system...

Abstract The cooperation between the actin and microtubule (MT) cytoskeletons is important for cellular processes such as cell migration muscle development. Full understanding of how this occurs has yet to be sufficiently developed. MT plus-end tracking protein (+TIP) CLIP-170 been implicated in actin-MT coordination by associating with actin-binding signaling IQGAP1, promoting polymerization through binding formins. Thus far, CLIP-170’s interactions were assumed indirect. Here, we...

Nonprofit property tax exemption is a major policy issue as local governments face growing budgetary pressures because of the recession and decreased revenue. Few empirical studies investigate why government officials favor or oppose PILOTs (payments in lieu taxes) their communities. Even fewer consider SILOTs – services taxes. Using data from survey Indiana officials, we examine whether opinions on PILOT policies are related to officials’ personal involvement with nonprofits; views...

ABSTRACT Portable and inexpensive analytical tools are required to monitor pharmaceutical quality in technology limited settings including low- middle-income countries (LMICs). Whole cell yeast biosensors have the potential help meet this need. However, most of read-outs for require expensive equipment or reagents. To overcome challenge, we designed a biosensor that produces unique scent as readout. This inducible biosensor, “scentsor,” does not user administer additional reagents reporter...