A5087210619- Plant Stress Responses and Tolerance
- Plant Gene Expression Analysis
- Plant Molecular Biology Research
- Photosynthetic Processes and Mechanisms
- Plant tissue culture and regeneration
- Plant-Microbe Interactions and Immunity
- Genetic Mapping and Diversity in Plants and Animals
- Plant nutrient uptake and metabolism
- Algal biology and biofuel production
- Plant responses to water stress
- Plant Genetic and Mutation Studies
- Legume Nitrogen Fixing Symbiosis
- Genomics and Phylogenetic Studies
- Plant biochemistry and biosynthesis
- Biochemical and biochemical processes
- Pesticide and Herbicide Environmental Studies
- Enzyme-mediated dye degradation
- GABA and Rice Research
- Plant Micronutrient Interactions and Effects
- Polysaccharides and Plant Cell Walls
- Plant-based Medicinal Research
- Aluminum toxicity and tolerance in plants and animals
- Polymer-Based Agricultural Enhancements
- Polyamine Metabolism and Applications
- Light effects on plants
Northeast Forestry University
2014-2025
Northeast Institute of Geography and Agroecology
2014-2023
Chinese Academy of Sciences
2014-2023
Shenyang Agricultural University
2023
Abstract In rice (Oryza sativa) and other plants, plant architecture seed size are closely related to yield. Brassinosteroid (BR) signaling the mitogen-activated protein kinase (MAPK) pathway (MAPK 10 [MAPKKK10]–MAPK 4 [MAPKK4]–MAPK6) two major regulatory pathways that control size. However, their possible relationship crosstalk remain elusive. Here, we show WRKY53 mediated between BR MAPK pathway. Biochemical genetic assays demonstrated glycogen synthase kinase-2 (GSK2) phosphorylates...
Abstract Cold tolerance at the booting (CTB) stage is a major factor limiting rice (Oryza sativa L.) productivity and geographical distribution. A few cold-tolerance genes have been identified, but they either need to be overexpressed result in CTB or cause yield penalties, their utility for breeding. Here, we characterize function of cold-induced transcription WRKY53 rice. The wrky53 mutant displays increased CTB, as determined by higher seed setting. Low temperature associated with lower...
Salinity is a major abiotic stress that seriously affects crop growth worldwide. In this work, we aimed to isolate potential halotolerant plant growth-promoting rhizobacteria (PGPR) mitigate the adverse impacts of salt in rice. An isolate, D2, with multiple (PGP) characteristics was identified as Enterobacter asburiae D2. Strain D2 could produce indole-3-acetic acid and siderophore. It also exhibited phosphate solubilization 1-aminocyclopropane-1-carboxylic deaminase activity. Genome...
The 636-bp-long cDNA sequence of OsCu/Zn-SOD (AK059841) was cloned from Oryza sativa var. Longjing11 via reverse transcription polymerase chain reaction (RT-PCR). encoded protein comprised 211 amino acids is highly homologous to Cu/Zn-SOD proteins tuscacera rice and millet. Quantitative RT-PCR revealed that in rice, the level gene expression lowest roots highest petals during S5 leaf stage. Moreover, decreased L5 stage maturity. expression, however, increased under saline–sodic stress NaHCO3...
Soil salinization severely inhibits plant growth, leading to a low crop yield. The aim of the current study was isolate endophytic bacteria with ability promote rice growth under saline conditions.We isolated eight salt-tolerant from roots. An strain D1 selected due its stimulate seed germination in presence NaCl, which identified as Pantoea ananatis D1. It exhibited multiple growth-promoting traits including phosphate solubilization, production indole-3-acetic acid,...
Grain size and leaf angle are key agronomic traits that determine final yields in rice. However, the underlying molecular mechanisms not well understood. Here we demonstrate Oryza sativa Mitogen Activated Protein Kinase OsMKKK70 regulates grain Overexpressing caused plants to produce longer seeds. The osmkkk62/70 double mutant osmkkk55/62/70 triple displayed significantly smaller seeds a more erect compared wild type, indicating functions redundantly with its homologs OsMKKK62 OsMKKK55....
ABSTRACT Panicle exsertion is one of the crucial agronomic traits in rice ( Oryza sativa ). Shortening panicle often leads to enclosure and severely reduces seed production. Gibberellin (GA) plays important roles regulating exsertion. However, underlying mechanism relative regulatory network remain elusive. Here, we characterized oswrky78 mutant showing severe enclosure, found that defect caused by decreased bioactive GA contents. Biochemical analysis demonstrates OsWRKY78 can directly...
Soil salinization is a worldwide environmental problem, especially in the arid and semiarid regions of northeastern China, which are heavily affected by soda saline-alkaline stress. At present, there an urgent need to improve stress tolerance rice.Stress-associated proteins involved regulating abiotic stresses plants. There 18 members rice stress-associated protein (OsSAP) gene family. In this study, expression levels OsSAP6 leaves roots were upregulated with increasing NaHCO3 duration. was...
Abstract Background WRKY transcription factors (TFs) have been suggested to play crucial roles in the response biotic and abiotic stresses. This study is first report alkaline salt regulation of gene. Results In this study, we cloned a gene ( SlWRKY28 ) from Salix linearistipularis then transferred Populus davidiana × P. bolleana for expression. Sequence analysis on transcriptome linearistipular showed significant up-regulation expression salt-alkali stress seedlings. Our data that localized...
Drought and soil salinization significantly constrain agricultural productivity, driving the need for molecular breeding strategies to enhance stress resistance. Zinc finger proteins play a critical role in plant response abiotic stress. In this study, gene encoding C2H2-type zinc protein (AfZFP5) was cloned from Amorpha fruticosa, species known its strong adaptability. qRT-PCR analysis revealed that AfZFP5 expression is regulated by sorbitol, H2O2, NaCl, NaHCO3. And all four treatments can...
The OsAPx2 gene from rice was cloned to produce PBI121::OsAPx2 dual-expression plants, of which expression level would be increasing under stressful conditions. enzyme ascorbate peroxidase (APX) in the leaves and roots plants increased with exposure time different sodium chloride (NaCl) hydrogen peroxide (H2O2)concentrations, as indicated by protein gel blot analysis. yield improved ability resist stress treatments. localized cytoplasm epidermal onion cells instantaneous green fluorescence....
Abstract Lipoxygenase (LOX, EC1.13.11.12) catalyzes the oxygenation of polyunsaturated fatty acids and further generates oxylipins via enzymatic or non-enzymatic pathways, thus participating in regulation growth development, stress response, other processes. The objective this research was to characterize function evaluate mechanism lipoxygenase gene AfLOX4 ’s role enhancing drought tolerance. Transcriptomic data were generated analyzed for LOX gene, under different concentrations...
Phenylalanine ammonia-lyase (PAL, E.C.4.3.1.5) catalyzes the benzene propane metabolism and is most extensively studied enzyme of phenylpropanoid pathway. However, role PAL genes in Astragalus membranaceus, a non-model plant showing high capability toward abiotic stress, less studied. Here, we cloned AmPAL found that it encodes protein resides cytoplasmic membrane. The mRNA was strongly induced by NaCl or NaHCO3 treatment, especially root. Overexpressing Nicotiana tabacum resulted higher...
The WRKY gene family in plants regulates the plant’s response to drought through regulatory networks and hormone signaling. AfWRKY20 (MT859405) was cloned from Amorpha fruticosa (A. fruticosa) seedlings using RT-PCR. binding properties of protein W-box (a DNA cis-acting element) were verified both vivo vitro EMSA Dual-Luciferase activity assays. RT-qPCR detected that total expression level leaves roots 22 times higher 30% PEG6000 simulated treatment compared untreated group. Under stress...
Flowering time is a fundamental factor determining the global distribution and final yield of rice (Oryza sativa). Although diverse flowering genes have been reported in this crop, transcriptional regulation its key are poorly understood. Here, we report that basic leucine zipper transcription factor, bZIP71, functions as repressor. The overexpression bZIP71 delays flowering, while bzip71 mutant flowers early both long-day short-day conditions. A genetic analysis showed by might be...
Mitogen-activated protein kinase (MAPK) is one of the factors cascade reactions affecting responses to signal pathway environmental stimuli. Throughout life plants, MAPK family members participate in transduction pathways and regulate various intracellular physiological metabolic reactions. To gain insights into regulatory function (MAPKK) Populus trichocarpa under salt stress, we obtained full-length cDNA PtMAPKK4 analyzed different expression levels gene leaves, stems, root organs. The...