A5002472661- Microtubule and mitosis dynamics
- Chromosomal and Genetic Variations
- Protist diversity and phylogeny
- Plant Molecular Biology Research
- Cancer Genomics and Diagnostics
- Photosynthetic Processes and Mechanisms
- Genomics and Chromatin Dynamics
- Pluripotent Stem Cells Research
- Viral Infectious Diseases and Gene Expression in Insects
- CRISPR and Genetic Engineering
Oncode Institute
2020-2023
University Medical Center Utrecht
2017-2023
Utrecht University
2020-2022
Chromosome segregation errors during cell divisions generate aneuploidies and micronuclei, which can undergo extensive chromosomal rearrangements such as chromothripsis1-5. Selective pressures then shape distinct aneuploidy rearrangement patterns-for example, in cancer6,7-but it is unknown whether initial biases micronucleation exist for particular chromosomes. Using single-cell DNA sequencing8 after an error-prone mitosis untransformed, diploid lines organoids, we show that chromosomes have...
Aurora B kinase is essential for faithful chromosome segregation during mitosis. During (pro)metaphase, concentrated at the inner centromere by kinases Haspin and Bub1. However, how Bub1 collaborate to control activity centromeres remains unclear. Here, we show that either or sufficient recruit a distinct chromosomal locus. Moreover, identified small, kinase-dependent pool supported in otherwise unchallenged cells. Joined inhibition of activities fully abolished accumulation centromeres....
Abstract Various cancer types exhibit characteristic and recurrent aneuploidy patterns. The origins of these type‐specific karyotypes are still unknown, partly because introducing or eliminating specific chromosomes in human cells poses a challenge. Here, we describe novel strategy to induce mis‐segregation different cell types. We employed Tet repressor nuclease‐dead Cas9 link microtubule minus‐end‐directed kinesin (Kinesin14VIb) from Physcomitrella patens integrated operon repeats...
The CRISPR/Cas9 system is a highly effective tool for genome editing. Key to robust editing the efficient delivery of machinery. Viral systems are vehicles transduction foreign genes but commonly used viral vectors suffer from limited capacity in genetic information they can carry. Baculovirus however capable carrying large exogenous DNA fragments. Here we investigate use baculoviral as vehicle based genome-editing tools. We demonstrate panel cell lines with Cas9 and an sgRNA sequence, which...
Abstract Various cancer types exhibit highly characteristic and recurrent aneuploidy patterns. The origin of these type-specific karyotypes, the extent to which they contribute progression, remains be elucidated, partly because introducing or eliminating specific chromosomes in human cells still poses a challenge. Here, we describe novel strategy mis-segregate at will different cell types. We employed Tet repressor (TetR) nuclease dead Cas9 (dCas9) link plant-derived microtubule...