Insulin-like growth factor II (IGF-II) is present at high levels in fetal and early neonatal rat plasma, decreases profoundly following birth. In the study, of IGF-II RNA different tissues ages were determined by hybridization to a cDNA probe. was 11 13 or examined: higher muscle, skin, lung, liver, intestine, thymus; lower brain stem, heart, cerebral cortex, kidney, hypothalamus; undetectable spleen pancreas (although latter partially degraded). each tissue, Northern blot revealed presence...

Insulin-like growth factor I (IGF-I) is a mitogenic polypeptide present in the plasma of man and rat that thought to mediate actions pituitary hormone on cartilage promote skeletal elongation.In rat, levels IGF-I show both developmental hormonal regulation: are low at birth, increase with age, decreased hormone-deficient adult animals.The study demonstrates these changes reflect abundance RNA liver.A human cDNA probe hybridized multiple species liver sizes 8.6,4.6,3.2,2.1,...

1. Loss of 6 to 8 pereiopods or chelipeds triggers precocious molts in a number marine crabs including the green crab, Carcinus maenas, blue Callinectes sapidus, and fiddler crabs, Uca pugnax U. pugilator, but not spider Libinia emarginata. Mortality rates are negligible compared those animals induced molt by eyestalk removal. 2. Precocious call be elicited land Gecarcinus lateralis, loss 5 appendages (pereiopods chelipeds) all 10 inhibits molting. cheliped which may have mass ten times...

Insulin-like growth factor II (IGF-II) is a mitogenic polypeptide present in rat plasma at high levels during fetal and early postnatal life believed to play an important, although as yet undefined, role development. Both humans rats, expression of the IGF-II gene results appearance several mRNA species. In study, cDNA synthetic oligonucleotide probes were used isolate characterize from genomic libraries. The extends over 12 kilobase pairs contains two 5'-noncoding exons three protein-coding...

If the Bermuda land crab, Gecarcinus lateralis , loses numerous walking legs or both chelipeds, it undergoes almost immediate preparations for molting with attendant limb regeneration. Injections of arthropod-molting hormone, ecdysterone, have no effect in either intact animals those missing legs.

We have used the rat C6 glial cell line as a model system to study role of insulin-like growth factors (IGF) in neuroglial cells central nervous (CNS). Northern blot analysis RNA demonstrated presence IGF-I mRNA and undetectable IGF-II mRNA. IGF-binding protein (s), but not IGF-II, were detected cell-conditioned medium. The level was 1-4 ng/ml conditioned medium based on human standard. immunoreactive inhibited [125I] binding receptor chick embryo fibroblasts stimulated [3H] thymidine...

Insulin-like growth factor II (IGF-II) is a mitogenic polypeptide that thought to play role in fetal and development. To study the hormonal developmental regulation of IGF-II gene expression, we have isolated cDNA clone for rat (rIGF-II) from 12S [1.2-kilobase-pair (kbp)] fraction mRNA liver cell line (BRL-3A) directs cell-free synthesis pre-pro-rIGF-II. In present study, rIGF-II probe was used determine size RNA. Surprisingly, BRL-3A cells neonatal liver, hybridized under stringent...

Recent investigations carried out in our laboratory have shown for several animal DNAs that repetitive and nonrepetitive sequences are interspersed a generally ordered manner. The basic type of experiment on which this conclusion rests begins with the reassociation trace quantities long, labeled DNA fragments short, unlabeled present excess. mixture is incubated to low C0t, so only reassociated, then passed over hydroxyapatite. Long, radioactive bind hydroxyapatite contain sequence elements...

The major excreted protein of transformed mouse fibroblasts, a secreted, mannose 6-phosphate-containing glycoprotein, is induced in nontransformed cells by variety transforming agents, phorbol esters, and platelet-derived growth factor. We report here the molecular cloning cDNA encoding this demonstrate that its induction consequence enhanced mRNA levels for both tetradecanoyl acetate-treated 3T3 retroviruses or retroviral oncogenes. These results indicate tumor promoters transformation...

Long and short repetitive sequences of sea urchin DNA were prepared by reassoci-ation 2000 nucleotide long fragments to Cot 4 digestion with the single strand specific nuclease S1. The S1 resistant duplexes separated into fractions on Agarose A50. extent shared was studied reassociating a labeled preparation an excess unlabeled DNA. Less than 10% able reassociate Thus elements appear be principally independent sequence classes in Precisely four successive steps reassociation thermal...

The bioavailability and action of the insulin-like growth factors (IGFs) are determined by specific IGF-binding proteins (IGFBP) to which they complexed. Complementary DNA clones have been isolated that encode three related IGFBPs: human IGFBP-1 (hlGFBP-1), IGFBP-3 (hlGFBP-3), rat IGFBP-2 (rlGFBP-2). regulated differently in plasma, suggesting different functions. In order study molecular basis regulation IGFBPs, we identified a panel cell lines express single predominant binding protein...

The single, overriding event that occurs during all proecdysial periods in crustaceans is the synthesis of a new exoskeleton encompasses an enlarged animal when old shell cast off. Regeneration missing appendages and larval or puberty metamorphoses also occur proecdysis. Proecdysial have been divided into substages defined by occurrence specific events. Although number factors must be postulated to account for individual events, only molting hormone, 20-hydroxyecdysone, has identified...

Increased amounts of mouse mammary tumor virus (MMTV) proviral transcripts were found in RNA dot blots from MMTV-negative, C3H/Sm tumors which arose spontaneously or induced by hormonal chemical carcinogens both. Other probed with a long terminal repeat (LTR) probe showed that LTR (MMTV)-containing disproportionately represented these RNAs. Different segments the MMTV genome used sequential hybridizations to Northern determine relative sequence content and size transformed tissues, as...

The major excreted protein of transformed mouse fibroblasts, a secreted, mannose 6-phosphate-containing glycoprotein, is induced in nontransformed cells by variety transforming agents, phorbol esters, and platelet-derived growth factor. We report here the molecular cloning cDNA encoding this demonstrate that its induction consequence enhanced mRNA levels for both tetradecanoyl acetate-treated 3T3 retroviruses or retroviral oncogenes. These results indicate tumor promoters transformation...

A 64 year old woman with a long history of "drop attacks" and dizzy spells was found to have spontaneous hypoglycaemia. slowly enlarging pleural mass had been present for at least five years. At thoracotomy the (weight 1.7 kg) excised hypoglycaemia ceased. Histologically tumour fibroma, no features malignancy. Endocrine tests before surgery showed subnormal growth hormone response hypoglycaemia, reduced concentration serum insulin like factor I (IGF-I), an inappropriately high II (IGF-II)....

C3H/Sm mice have lost the exogenous milk-borne mouse mammary tumor virus (MMTV) characteristic of C3H strain and a very low (1.5%) incidence spontaneous tumors, yet they are highly susceptible to carcinogenesis by either chemical carcinogens or infection with virus. We analyzed MMTV proviral DNA content normal tissues spontaneous, virus-induced, chemically induced tumors restriction endonuclease digestion Southern blot analysis. Although results clearly showed additional sequences in...

Journal Article Isolation of discrete repetitive sequence classes from Xenopus DNA by high temperature reassociation Get access Barbara A. Braun, Braun Department Biological Sciences, Purdue UniversityWest Lafayette, IN 47907, USA Search for other works this author on: Oxford Academic PubMed Google Scholar Kathleen E. Schanke, Schanke Dale Graham Nucleic Acids Research, Volume 5, Issue 11, 1 November 1978, Pages 4283–4304, https://doi.org/10.1093/nar/5.11.4283 Published: 01 1978 history...