A5064222137- Tuberculosis Research and Epidemiology
- Bioinformatics and Genomic Networks
- Mycobacterium research and diagnosis
- Diet and metabolism studies
- Liver Disease Diagnosis and Treatment
- Tryptophan and brain disorders
- Gut microbiota and health
- Adipokines, Inflammation, and Metabolic Diseases
- Genomics and Phylogenetic Studies
- Metabolomics and Mass Spectrometry Studies
- Hops Chemistry and Applications
- Adipose Tissue and Metabolism
- Ginseng Biological Effects and Applications
- Bacteriophages and microbial interactions
- Computational Drug Discovery Methods
- Cancer-related molecular mechanisms research
- Peroxisome Proliferator-Activated Receptors
- Biochemical and Molecular Research
- vaccines and immunoinformatics approaches
- Histone Deacetylase Inhibitors Research
- Digestive system and related health
- Quinazolinone synthesis and applications
- Sirtuins and Resveratrol in Medicine
- Lipid metabolism and disorders
- Gene Regulatory Network Analysis
Oregon State University
2022-2024
Indian Institute of Science Bangalore
2010-2016
University of Cologne
2005
IL-22 is critical for ameliorating obesity-induced metabolic disorders. However, it unknown where acts to mediate these outcomes. Here we examine the importance of tissue-specific IL-22RA1 signaling in mediating long-term high fat diet (HFD) driven To do so, generated intestinal epithelium-, liver-, and white adipose tissue (WAT)-specific Il22ra1 knockout littermate control mice. Intestinal epithelium- liver-specific upregulated systemic glucose metabolism. also mediated liver WAT metabolism...
Microbiota contribute to the induction of type 2 diabetes by high-fat/high-sugar (HFHS) diet, but which organs/pathways are impacted microbiota remain unknown. Using multiorgan network and transkingdom analyses, we found that microbiota-dependent impairment OXPHOS/mitochondria in white adipose tissue (WAT) plays a primary role regulating systemic glucose metabolism. The follow-up analysis established Mmp12+ macrophages link inflammation OXPHOS damage WAT. Moreover, molecular signature WAT...
Abstract Background The gut microbiota contributes to macrophage-mediated inflammation in adipose tissue with consumption of an obesogenic diet, thus driving the development metabolic syndrome. There is a need identify and develop interventions that abrogate this condition. hops-derived prenylated flavonoid xanthohumol (XN) its semi-synthetic derivative tetrahydroxanthohumol (TXN) attenuate high-fat diet-induced obesity, hepatosteatosis, syndrome C57Bl/6J mice. This coincides decrease...
We report the whole genome sequences of a Mycobacterium smegmatis laboratory wild-type strain (MC(2) 155) and mutants (4XR1, 4XR2) resistant to isoniazid. Compared MC(2) 155 (NC_008596), widely used in experiments, 155, 4XR1, 4XR2 strains are 60, 128 93 bp longer, respectively.
The global mechanisms and associated molecular alterations that occur in drug-resistant mycobacteria are poorly understood. To address this, we obtain genomics data then construct a genome-scale response network isoniazid-resistant Mycobacterium smegmatis apply network-mining algorithm. Through decipher an unbiased manner identify emergent vulnerabilities resistant bacilli, of which redox was prominent. Using phenotypic profiling, find bacilli exhibit collateral sensitivity to several...
Many studies on M. tuberculosis have emerged from using smegmatis MC 2 155 (Msm), since they share significant similarities and yet Msm is non-pathogenic faster growing. Although several individual molecules been studied Msm, many questions remain open about its metabolism as a whole capability to be versatile. Adaptability versatility are emergent properties of system, warranting molecular systems perspective understand them. We identify feasible metabolic pathways in reference condition...
Nonalcoholic fatty liver disease (NAFLD) is a global health problem. Identifying early gene indicators contributing to the onset and progression of NAFLD has potential develop novel targets for therapeutic intervention. We report on late transcriptomic signatures western diet (WD)-induced nonalcoholic steatohepatitis (NASH) in female male
The biosynthesis of NAD constitutes an important metabolic module in the cell, since is essential cofactor involved several reactions. concentrations are known to be significantly increased cancers, particularly glioma, consistent with observation up-regulation enzymes network. Modulating glioma therefore attractive therapeutic strategy. Here we report reconstruction a biochemical network consisting 22 proteins, 36 metabolites, and 86 parameters, tuned mimic conditions glioma. Kinetic...
Resistance to therapy limits the effectiveness of drug treatment in many diseases. Drug resistance can be considered as a successful outcome bacterial struggle survive hostile environment drug-exposed cell. An important mechanism by which bacteria acquire is through mutations target. resistant strains (multi-drug and extensively resistant) Mycobacterium tuberculosis are being identified at alarming rates, increasing global burden tuberculosis. understanding nature different targets how they...
The global variations in the gene expression pattern of drug treated (½X) and laboratory evolved drug-resistant strains (2XR 4XR) Mycobacterium smegmatis were obtained compared with M. mc(2) 155 (WT) strain. genes exhibiting two-fold change p-value <0.05 under conditions have been considered as differentially expressed (DEGs). Overall, numbers DEGs observed are 1529 ½X (596-up, 933-down), 1381 (899-up, 482-down) 2XR 716 4XR (267-up, 449-down) conditions. data is publicly available through...
metabolic pathway alignmentload pointchoke pointdrug targetpathway analysisconserved pathwaysalternate
Technological advances have generated tremendous amounts of high-throughput omics data. Integrating data from multiple cohorts and diverse types new previously published studies can offer a holistic view biological system aid in deciphering its critical players key mechanisms. In this protocol, we describe how to use Transkingdom Network Analysis (TkNA), unique causal-inference analytical framework that perform meta-analysis detect master regulators among measured parameters govern...
Abstract Clinical and preclinical studies have established that supplementing diets with ω3 polyunsaturated fatty acids (PUFA) can reduce hepatic dysfunction in nonalcoholic steatohepatitis (NASH). Herein, we used multi-omic network analysis to unveil novel mechanistic targets of PUFA effects a mouse model western diet induced NASH. After identifying critical molecular processes responsible for the PUFA, next performed meta-analysis human liver cancer transcriptomes uncovered betacellulin as...
Abstract The gut microbiota contributes to macrophage-mediated inflammation in adipose tissue with consumption of an obesogenic diet, thus driving the development metabolic syndrome. There is a need identify and develop interventions that abrogate this condition. hops-derived prenylated flavonoid xanthohumol (XN) its semi-synthetic derivative tetrahydroxanthohumol (TXN) attenuate high-fat diet-induced obesity, hepatosteatosis syndrome C57Bl/6J mice. This coincides decrease pro-inflammatory...