A5038679251- Trace Elements in Health
- Iron Metabolism and Disorders
- Hemoglobinopathies and Related Disorders
- Animal Nutrition and Physiology
- Heavy Metal Exposure and Toxicity
- Erythrocyte Function and Pathophysiology
- Porphyrin Metabolism and Disorders
- Genetic Associations and Epidemiology
- RNA modifications and cancer
- Plant Micronutrient Interactions and Effects
- Cancer-related gene regulation
- Animal Behavior and Welfare Studies
- RNA regulation and disease
- Endoplasmic Reticulum Stress and Disease
- MicroRNA in disease regulation
- Pregnancy and Medication Impact
- Child Nutrition and Water Access
- Calcium signaling and nucleotide metabolism
- Aluminum toxicity and tolerance in plants and animals
- Enzyme Structure and Function
- Ferroptosis and cancer prognosis
- Hydrogen Storage and Materials
- GABA and Rice Research
- Autophagy in Disease and Therapy
- Pharmacological Effects of Medicinal Plants
Yonsei University
2022-2024
University of Minnesota
2017-2023
University of Minnesota System
2019-2021
National Institutes of Health
2014-2017
National Institute of Diabetes and Digestive and Kidney Diseases
2014-2017
University of Florida
2007-2012
ZIP14 (slc39A14) is a zinc transporter induced in response to pro-inflammatory stimuli. induction accompanies the reduction serum (hypozincemia) of acute inflammation. can transport Zn2+ and non-transferrin-bound Fe2+ vitro. Using Zip14−/− mouse model we demonstrated that was essential for control phosphatase PTP1B activity phosphorylation c-Met during liver regeneration. In current studies, global screening ZIP gene expression LPS-induced endotoxemia conducted. Following LPS, Zip14 most...
Developing erythrocytes take up exceptionally large amounts of iron, which must be transferred to mitochondria for incorporation into heme. This massive iron flux precisely controlled permit the coordinated synthesis heme and hemoglobin while avoiding toxic effects chemically reactive iron. In cultured animal cells, chaperones poly rC–binding protein 1 (PCBP1) PCBP2 deliver ferritin, sole cytosolic storage protein, nuclear receptor coactivator 4 (NCOA4) mediates autophagic turnover ferritin....
Significance One-third of the proteins expressed in eukaryotic and prokaryotic cells may require bound metal ions or cofactors for activity. In human cells, processes by which acquire their native avoid mismetallation are largely unknown. The multifunctional adaptor poly(rC)-binding protein (PCBP)1 PCBP2 iron chaperones that deliver ferrous via a metal-mediated, protein–protein interaction. Three PCBP targets have been identified: ferritin, an storage protein; prolyl asparagyl hydroxylase,...
Implementation of zinc interventions for subjects suspected being zinc-deficient is a global need, but limited due to the absence reliable biomarkers. To discover molecular signatures human deficiency, combination transcriptome, cytokine, and microRNA analyses was applied dietary depletion/repletion protocol with young male subjects. Concomitant decrease in serum concentration, changes buccal blood gene transcripts related homeostasis occurred depletion. Microarray whole RNA revealed...
The exocrine pancreas plays an important role in endogenous zinc loss by regulating excretion into the intestinal tract and hence influences dietary requirement. present experiments show that transporter ZnT2 (Slc30a2) is localized to zymogen granules restriction mice decreased concentration of expression. Excess given orally increased expression was associated with pancreatic accumulation. Rat AR42J acinar cells when induced a secretory phenotype, using glucocorticoid analog dexamethasone...
The regulation of cellular zinc uptake is a key process in the overall mechanism governing mammalian homeostasis and how participates functions. We analyzed transporters Zip family both brain liver zinc-deficient animals found large, significant increase Zip10 expression. Additionally, expression decreased response to repletion. Moreover, isolated mouse hepatocytes, AML12 Neuro 2A cells also respond differentially availability vitro. Measurement hnRNA actinomycin D inhibition studies...
Anemia is the most prevalent nutrition-related disorder worldwide. Zinc an essential trace element for various biological processes in body, and zinc deficiency has been associated with anemia humans. However, molecular mechanisms by which availability alters red blood cell development remain uncertain. The present study identifies essentiality of during erythroid development, particularly normal heme biosynthesis. G1E-ER4 mouse cells were used as vitro model terminal differentiation,...
Sows were supplemented with high dietary zinc (Zn) at different times in gestation to determine effects on piglet tissue mineral concentrations and gene expression. Ten sSows per treatment assigned treatments: 1) Control - sows fed a corn-soybean meal-based diet containing 206 ppm total supplemental supplied by hydroxychloride from mating farrowing; 2) Breed-to-Farrow as + additional 147 Zn ZnSO4 5 days post-breeding 3) Day 110-to-Farrow 4,079 starting day 110 of until farrowing. Final the...
Blood levels of iron and copper, even within their normal ranges, have been associated with a wide range clinical outcomes. The available epidemiological evidence for these associations is often inconsistent suffers from confounding reverse causation. This study aims to examine the causal effects blood copper Mendelian randomization (MR) analyses. Genetic instruments were curated existing genome-wide association studies. Candidate outcomes identified based on phenome-wide (PheWAS) between...
Abstract Data from small clinical trials in the United States and India suggest zinc supplementation reduces infection adolescents adults with sickle cell anemia (SCA), but no studies of for prevention have been conducted children SCA living Africa. We a randomized double-blind placebo-controlled trial to assess severe or invasive infections Ugandan 1.00-4.99 years SCA. Of 252 enrolled participants, 124 were assigned (10 mg) 126 placebo once daily 12 months. The primary outcome was incidence...
Neurons require iron to support their metabolism, growth, and differentiation, but are also susceptible iron-induced oxidative stress cytotoxicity. Ferritin, a cytosolic storage unit, mediates cellular adaptation fluctuations in delivery. NCOA4 has been characterized as selective autophagic cargo receptor facilitating the mobilization of intracellular from ferritin. This process named ferritinophagy results degradation ferritin consequent release into cytosol.Here we demonstrate that is...
Macrophages, via erythrophagocytosis, recycle iron from effete erythrocytes to newly developing red blood cells. Conversion of potentially cytotoxic levels its heme into nonheme form during recycling is safely accomplished coordinated regulations cellular transport and homeostasis. Herein, we demonstrate the roles regulation NCOA4 (nuclear receptor coactivator 4)-mediated ferritinophagy in macrophages after erythrophagocytosis using mouse macrophage cell line J774 Ferritin cells increased...
Abstract The objectives of this study were to determine a practical approach feeding elevated dietary zinc (Zn) gestating sows in commercial setting and confirm preweaning mortality could be reduced by high Zn during different periods gestation. was conducted at sow farm the upper Midwest. Mixed parity (n = 267) over three consecutive weekly farrowing groups (sows within 1 wk) assigned randomly one treatments parity. Treatments consisted of: (1) control fed corn–soybean meal diet containing...
Abstract The objective of this study was to assess if high dietary zinc (Zn) fed gestating sows modulates the microbiome sow and that their offspring. Sows were 1 3 treatments: 1) Control: a corn-soybean meal-based diet containing 125 ppm total supplemental zinc, 2) Breed-to-Farrow: as Control + 141 Zn ZnSO4 from 5 d post-breeding farrowing; 3) Day 110-to-Farrow: 2,715 starting on 110 gestation until farrowing. A subset third parity (n = 30; 10 per treatment) selected colostrum, milk, rectal...
Abstract Background Blood levels of iron and copper, even within their normal ranges, have been associated with a wide range clinical outcomes. Available epidemiological evidence on blood copper association potential effects, such as lipid metabolism disorder, is inconsistent scarce. This study aims to examine disentangle the causal effects copper. Methods Genetic instruments for level were curated from existing genome-wide studies. Candidate outcomes identified based phenome-wide (PheWAS)...