A5042911414- DNA and Nucleic Acid Chemistry
- Crystallization and Solubility Studies
- X-ray Diffraction in Crystallography
- Chemical Synthesis and Analysis
- Supramolecular Self-Assembly in Materials
- Advanced biosensing and bioanalysis techniques
- Light effects on plants
- Photochromic and Fluorescence Chemistry
- Synthesis and Characterization of Heterocyclic Compounds
- Click Chemistry and Applications
- Membrane Separation and Gas Transport
- RNA Interference and Gene Delivery
- Carbon and Quantum Dots Applications
- Nanoparticle-Based Drug Delivery
- Alzheimer's disease research and treatments
- Carbon Dioxide Capture Technologies
- Computational Drug Discovery Methods
- Microbial Metabolism and Applications
- Synthesis and Biological Evaluation
- Nanocluster Synthesis and Applications
- Enzyme-mediated dye degradation
- Microbial bioremediation and biosurfactants
- RNA and protein synthesis mechanisms
- Membrane Separation Technologies
- Nanoplatforms for cancer theranostics
Indian Institute of Technology Guwahati
2013-2024
RIKEN Advanced Science Institute
2012
University of Alberta
2006
Ludwig-Maximilians-Universität München
2004
Philipps University of Marburg
2001-2002
DNA and RNA hairpins, which represent well-folded oligonucleotide structures, were irradiated the amount of damaged hairpins was directly quantified by using ion-exchange HPLC. The types photoproducts formed in determined ESI-HPLC-MS/MS experiments. Irradiation with systematically varied sequences conformations (A versus B) revealed remarkable differences regarding photolesions formed. UV-damage formation is, therefore, a strongly sequence conformation dependent process.
In order to gain deeper insight into the function and interplay of proteins in cells it is essential develop methods that allow profiling protein real time, solution, cells, cell organelles. Here we report development a U-type oligonucleotide (molecular beacon) contains fluorophore quencher at tips, addition substrate analogue loop structure. This induces hairpin cleavage response enzyme action, which translated fluorescence signal. The molecular beacon developed here was used characterize...
An affinity capillary electrophoresis method was developed to determine a binding constant between peptide nucleic acid (PNA) and hairpin-structured DNA. A diblock copolymer composed of PNA polyethylene glycol (PEG) synthesized as novel probe. The base sequence the probe's segment complementary region 60-base single-stranded DNA (ssDNA). Upon applying voltage, hairpin migrated slowly compared random ssDNA in presence This retardation induced by strand invasion into form hybridized complex,...
In order to gain deeper insight into the function and interplay of proteins in cells it is essential develop methods that allow profiling protein real time, solution, cells, cell organelles. Here we report development a U-type oligonucleotide (molecular beacon) contains fluorophore quencher at tips, addition substrate analogue loop structure. This induces hairpin cleavage response enzyme action, which translated fluorescence signal. The molecular beacon developed here was used characterize...
Strategically designed, heteroatom-rich surface functionalized blue fluorescent carbon dots (CDs) were synthesized for high-throughput detection of folic acid (vitamin B9). The highly stable CDs could particularly detect vitamin B9 in the presence 35 analytes, even up to 40 nM vitamin. versatile found have a high affinity wastewater, tablets, and food samples enriched with acid. hemocompatibility was also studied by using hemolysis assay, confirming be nontoxic human blood 400 μg/mL. then...
8-oxo-deoxyguanosine (8-oxo-dG) is a major oxidative lesion in DNA and responsible for mutation cancer. Current techniques detecting 8-oxo-dG are indirect methods. Thus, development of new methodologies needed to directly detect such lesions. In this article, we have used ultraviolet resonance Raman (UVRR) spectroscopy as novel analytical technique the detection 8-oxo-dG. Here, UVRR spectrum was acquired compared that deoxyguanosine (dG) deoxyadenosine (dA). Data analysis shows distinct with...
A series of 2-aminopyrimidine derivatives, substituted at 5- and 6-positions, were synthesized.
Abstract Widely used chemotherapeutic agent 5‐fluorouracil (5‐Fu) exhibits adverse effects by damaging the normal cells. Controlled delivery and release of such antitumor drugs is essential for better treatment. Herein, a low molecular weight peptide‐drug conjugate photo‐controlled 5‐Fu demonstrated. covalently attached with short peptide through photo‐cleavable linker. With careful choice sequence, was able to form hydrogel within narrow pH window (pH 6.0 ‐ 8.0). MTT assay in HeLa cells...