A5005698702- Electrospun Nanofibers in Biomedical Applications
- Cardiac Valve Diseases and Treatments
- Cellular Mechanics and Interactions
- Tissue Engineering and Regenerative Medicine
- Pressure Ulcer Prevention and Management
- Elasticity and Material Modeling
- 3D Printing in Biomedical Research
- Diabetic Foot Ulcer Assessment and Management
- Wound Healing and Treatments
- Bone Tissue Engineering Materials
- Angiogenesis and VEGF in Cancer
- Nitric Oxide and Endothelin Effects
- Cardiac and Coronary Surgery Techniques
- Tendon Structure and Treatment
- Coronary Interventions and Diagnostics
- Orthopaedic implants and arthroplasty
- Cardiovascular Function and Risk Factors
- History and Theory of Mathematics
- Hippo pathway signaling and YAP/TAZ
- Cardiac Structural Anomalies and Repair
- Tribology and Lubrication Engineering
- Cell Adhesion Molecules Research
- Renal and related cancers
- Skin and Cellular Biology Research
- Connective tissue disorders research
Eindhoven University of Technology
2016-2025
Institute for Complex Systems
2017
Cytograft Tissue Engineering (United States)
2009-2015
Computational modeling–inspired heart valve designs guide tissue remodeling and ensure long-term functionality in tissue-engineered valves sheep.
Deep tissue injury (DTI) is a severe form of pressure ulcer where damage starts in deep tissues underneath intact skin. In the present study, contributions deformation, ischemia, and reperfusion to skeletal muscle development were examined rat model during 6-h period. Magnetic resonance imaging (MRI) was used study perfusion (contrast-enhanced MRI) integrity (T2-weighted MRI). The levels deformation estimated using finite element models. Complete ischemia caused gradual homogeneous increase...
Abstract The intermediate filament (IF) cytoskeleton has been proposed to regulate morphogenic processes by integrating the cell fate signaling machinery with mechanical cues. Signaling between endothelial cells (ECs) and vascular smooth muscle (VSMCs) through Notch pathway regulates arterial remodeling in response changes blood flow. Here we show that IF-protein vimentin strength hemodynamic forces. Vimentin is important for transactivation ECs knockout mice (VimKO) display disrupted VSMC...
Mechanical loading of soft tissues covering bony prominences can cause skeletal muscle damage, ultimately resulting in a severe pressure ulcer termed deep tissue injury. Recently, by means an experimental-numerical approach, it was shown that local deformations damage once deformation threshold is exceeded. In the present study, effects load exposure time and intermittent relief on development deformation-induced were investigated. The data showed 2 h period caused more than 10 min loading....
Tissue engineering represents a promising technique to overcome the limitations of current valve replacements, since it allows for creating living autologous heart valves that have potential grow and remodel. However, also this approach still faces number challenges. One particular problem is regurgitation, caused by cell-mediated tissue retraction or mismatch in geometrical material properties between tissue-engineered (TEHVs) their native counterparts. The goal present study was assess...
In the presence of anisotropic biochemical or topographical patterns, cells tend to align in direction these cues-a widely reported phenomenon known as "contact guidance." To investigate origins contact guidance, here, we created substrates micropatterned with parallel lines fibronectin dimensions spanning multiple orders magnitude. Quantitative morphometric analysis our experimental data reveals two regimes guidance governed by length scale cues that cannot be explained enforced alignment...
Continuous flow ventricular assist devices (VADs) for mechanical circulatory support (MCS) are generally smaller and believed to be more reliable than pulsatile VADs. However, regarding continuous flow, there concerns about the decreased pulsatility unloading. Moreover, VADs offer a wider range in control strategies. For this reason, we used computer model evaluate whether operation of VAD would beneficial standard constant pump speed. The describes left right ventricle with one-fiber heart...
In the cardiac microenvironment, cardiomyocytes (CMs) are embedded in an aligned and structured extracellular matrix (ECM) to maintain coordinated contractile function of heart. The fibroblast (cFB) is main cell type responsible for producing remodeling this matrix. diseases, however, adverse CM death may lead deterioration myocardial structure. Here, we present vitro model system with uniaxial biaxial constraints induce (an)isotropy 3D microtissues, thereby mimicking ‘healthy’ ‘diseased’...
One of the most critical problems in heart valve tissue engineering is progressive development valvular insufficiency due to leaflet retraction. Understanding underlying mechanisms this process crucial for developing tissue-engineered valves (TEHVs) that maintain their functionality long term. In present study, we adopted a computational approach predict remodeling TEHVs subjected dynamic pulmonary and aortic pressure conditions, assess risk insufficiency. addition, investigated importance...
Recent studies on decellularized tissue engineered heart valves (DTEHVs) showed rapid host cell repopulation and increased valvular insufficiency developing over time, associated with leaflet shortening. A possible explanation for this result was found using computational simulations, which revealed radial compression in the original geometry when subjected to physiological pressure conditions. Therefore, an improved suggested enable extension counteract mediated retraction. In study, we...
Significance Notch signaling and hemodynamics are widely known to regulate arterial morphogenesis, remodeling, homeostasis. Recent studies suggest that mechanics interact in vascular but the impact on homeostasis is still unclear. Here, using a computational–experimental approach, we show expression of ligands, receptors, target genes down-regulated with mechanical strain. Incorporation these results computational model wall reveals this mechanosensitivity leads sudden transition from...
Tendinopathy is a widespread and unresolved clinical challenge, in which associated pain hampered mobility present major cause for work-related disability. associates with change from healthy tissue aligned extracellular matrix (ECM) highly polarized cells that are connected head-to-tail, towards diseased disorganized ECM randomly distributed cells, scar-like features commonly attributed to poor innate regenerative capacity of the tissue. A fundamental dilemma this scarring process whether...
Epithelial cysts and organoids are multicellular hollow structures formed by correctly polarized epithelial cells. Important in steering these from single cells is the dynamic regulation of extracellular matrix presented ligands, dynamics. Here, control over effective ligand concentration introduced, decoupled bulk local mechanical properties, synthetic supramolecular hydrogels through noncovalent crosslinking fibers. Control realized 1) keeping constant, but changing nonfunctionalized...
Abstract Pressure ulcers are localized areas of soft tissue breakdown due to mechanical loading. Susceptible individuals subjected pressure relief strategies prevent long loading periods. Therefore, ischemia‐reperfusion injury may play an important role in the etiology ulcers. To investigate inter‐relation between postischemic perfusion and changes skeletal muscle integrity, hindlimbs Brown Norway rats were 4‐h ischemia followed by 2‐h reperfusion. Dynamic contrast‐enhanced MRI was used...
Abstract Regenerative tissue-engineered matrix-based heart valves (TEM-based TEHVs) may become an alternative to currently-used bioprostheses for transcatheter valve replacement. We recently identified TEM-based TEHVs-geometry as one key-factor guiding their remodeling towards successful long-term performance or failure. While our first-generation TEHVs, with a simple, non-physiological valve-geometry, failed over time due leaflet-wall fusion phenomena, second-generation computational...