A5052741873- Developmental Biology and Gene Regulation
- Nanoparticle-Based Drug Delivery
- Cellular Mechanics and Interactions
- 3D Printing in Biomedical Research
- Skin and Cellular Biology Research
- Cancer Cells and Metastasis
- Angiogenesis and VEGF in Cancer
- Cell Adhesion Molecules Research
- Tissue Engineering and Regenerative Medicine
- Cancer Genomics and Diagnostics
- Epigenetics and DNA Methylation
- Congenital heart defects research
- Graphene and Nanomaterials Applications
- Pluripotent Stem Cells Research
- RNA Interference and Gene Delivery
- Nanoplatforms for cancer theranostics
- Microfluidic and Bio-sensing Technologies
- Mesoporous Materials and Catalysis
- RNA Research and Splicing
- Muscle Physiology and Disorders
- Diamond and Carbon-based Materials Research
- Bone Tissue Engineering Materials
- Wnt/β-catenin signaling in development and cancer
- Cancer, Hypoxia, and Metabolism
- Cancer Mechanisms and Therapy
Åbo Akademi University
2016-2025
Eindhoven University of Technology
2016-2025
University of Turku
2015-2024
Turku Centre for Computer Science
2020-2024
Turku Centre for Biotechnology
2011-2023
Cytograft Tissue Engineering (United States)
2016-2018
Institute for Complex Systems
2016
In-Q-Tel
2016
Turku University of Applied Sciences
2015
Karolinska Institutet
2006-2010
Tumor hypoxia is linked to increased metastatic potential, but the molecular mechanisms coupling metastasis are poorly understood. Here, we show that Notch signaling required convert hypoxic stimulus into epithelial-mesenchymal transition (EMT), motility, and invasiveness. Inhibition of abrogated hypoxia-induced EMT invasion, and, conversely, an activated form could substitute for induce these processes. deploys two distinct act in synergy control expression Snail-1, a critical regulator...
Mesoporous silica nanoparticles functionalized by surface hyperbranching polymerization of poly(ethylene imine), PEI, were further modified introducing both fluorescent and targeting moieties, with the aim specifically cancer cells. Owing to high abundance folate receptors in many cells as compared normal cells, folic acid was used ligand. The internalization particles cell lines expressing different levels studied. Flow cytometry quantify mean number internalized per cell. Five times more...
Intermediate filaments are general constituents of the cytoskeleton. The function these structures and requirement for different types intermediate filament proteins by individual cells only partly understood. Here we have addressed role specific protein partnerships in formation astrocytes. Astrocytes may express three proteins: glial fibrillary acidic (GFAP), vimentin, nestin. We used mice with targeted mutations GFAP or vimentin genes, both, to study impact loss either both on cultured...
Targeted nanoparticle-mediated intracellular delivery is demonstrated using two hydrophobic fluorophores as model drug cargo. The presented hybrid carrier system exhibits both cancer cell-targeting ability and capacity to retain a agent with subsequent specific release into the endosomal compartment. Furthermore, incorporated shown be able escape from endosomes cytoplasm, making particles promising candidates carriers for targeted treatment.
Notch signaling, a key regulator of stem cells, is frequently overactivated in cancer. It often linked to aggressive forms cancer, evading standard treatment highlighting as an exciting therapeutic target. principle "druggable" by γ-secretase inhibitors (GSIs), inhibitory peptides and antibodies, but clinical use restricted severe side effects there demand for alternative cancer-targeted therapy. Here, we present novel approach, using imagable mesoporous silica nanoparticles (MSNPs) vehicles...
Notch signaling is critically important for proper architecture of the vascular system, and mutations in NOTCH3 are associated with CADASIL, a stroke dementia syndrome smooth muscle cell (VSMC) dysfunction. In this report, we link to platelet-derived growth factor (PDGF) signaling, key determinant VSMC biology, show that PDGF receptor ( PDGFR )-β novel immediate target gene. -β expression was upregulated by ligand induction or activated forms receptor. Moreover, upregulation response...
Targeted delivery of the chemotherapeutic agent methotrexate (MTX) to cancer cells using poly(ethyleneimine)-functionalized mesoporous silica particles as drug-delivery vectors is reported. Due its high affinity for folate receptors, expression which elevated in cells, MTX serves both a targeting ligand and cytotoxic agent. Enhanced cancer-cell apoptosis (programmed cell death) relative free thus observed at particle concentrations where nonspecific MTX-induced not nontargeted healthy line,...
Adult neurogenesis is regulated by a number of cellular players within the neurogenic niche. Astrocytes participate actively in brain development, regulation mature central nervous system (CNS), and plasticity. They are important regulators local environment adult niches through secretion diffusible morphogenic factors, such as Wnts. control niche also membrane-associated however, identity these factors mechanisms involved largely unknown. In this study, we sought to determine underlying our...
Notch signaling is frequently hyperactivated in breast cancer, but how the enhanced contributes to tumor process less well understood. In this report, we identify proinflammatory cytokine interleukin-6 (IL-6) as a novel target cells. Enhanced upregulated IL-6 expression, leading activation of autocrine and paracrine Janus kinase/signal transducers activators transcription signaling. upregulation was mediated by non-canonical signaling, it could be effectuated cytoplasmically localized...
A switch from oxidative phosphorylation to glycolysis is frequently observed in cancer cells and linked tumor growth invasion, but the underpinning molecular mechanisms controlling are poorly understood. In this report we show that Notch signaling a key regulator of cellular metabolism. Both hyper- hypoactivated induce glycolytic phenotype breast cells, although by distinct mechanisms: hyperactivated leads increased through activation phosphatidylinositol 3-kinase/AKT serine/threonine kinase...
Recent advances within materials science and its interdisciplinary applications in biomedicine have emphasized the potential of using a single multifunctional composite material for concurrent drug delivery biomedical imaging. Here we present novel consisting photoluminescent nanodiamond (ND) core with porous silica (SiO2) shell. This probe serves as an alternative nanomaterial to address existing problems subsequent tracing particles. Whereas unique optical properties ND allows long-term...
Cell signalling and mechanics influence vascular pathophysiology there is an increasing demand for<italic>in vitro</italic>model systems that enable examination of between cells under hemodynamic conditions.
Significance Angiogenesis, the formation of new blood vessels from existing ones, is essential for embryonic development and necessary tumor growth invasion. The Notch signaling pathway regulates angiogenesis where two ligands, Dll4 Jagged 1, exert opposite functions on sprouting. We found that intermediate filament vimentin balances by binding specifically to proangiogenic ligands. This provides a force-generating mechanism Jagged–Notch complex ensure efficient transendocytosis activation....
Cancer stem cells (CSCs) are a challenge in cancer treatment due to their therapy resistance. We demonstrated that enhanced Notch signaling breast promotes self-renewal of CSCs display high glycolytic activity and aggressive hormone-independent tumor growth vivo. took advantage the phenotype dependence on designed nanoparticles target CSCs. Mesoporous silica were functionalized with glucose moieties loaded γ-secretase inhibitor, potent interceptor signaling. vitro vivo efficiently...
Drug delivery into the brain is impeded by blood-brain-barrier (BBB) that filters out vast majority of drugs after systemic administration. In this work, we assessed transport, uptake and cytotoxicity promising drug nanocarriers, mesoporous silica nanoparticles (MSNs), in vitro models BBB. RBE4 rat endothelial cells Madin-Darby canine kidney epithelial cells, strain II, were used as BBB models. We studied spherical rod-shaped MSNs with following modifications: bare coated a poly(ethylene...