A5009876043- Cancer Genomics and Diagnostics
- Developmental Biology and Gene Regulation
- Cancer Cells and Metastasis
- Single-cell and spatial transcriptomics
- Monoclonal and Polyclonal Antibodies Research
- Advanced biosensing and bioanalysis techniques
- Congenital Anomalies and Fetal Surgery
- HER2/EGFR in Cancer Research
- Diabetic Foot Ulcer Assessment and Management
- Cancer-related molecular mechanisms research
- Extracellular vesicles in disease
- Wound Healing and Treatments
- Pediatric Hepatobiliary Diseases and Treatments
- Cytokine Signaling Pathways and Interactions
- Thermoregulation and physiological responses
- Adipose Tissue and Metabolism
- Sepsis Diagnosis and Treatment
- Gallbladder and Bile Duct Disorders
- Bacterial Identification and Susceptibility Testing
- PI3K/AKT/mTOR signaling in cancer
- Neonatal and Maternal Infections
- Immune cells in cancer
- Mesenchymal stem cell research
- NF-κB Signaling Pathways
- Molecular Biology Techniques and Applications
Integrated Cardio Metabolic Centre
2017-2020
AstraZeneca (Sweden)
2020
Karolinska Institutet
2012-2020
Biocon (India)
2012
Global Hospitals
2012
The Ohio State University
2005
The Ohio State University Wexner Medical Center
2005
Abstract Many important cell types in adult vertebrates have a mesenchymal origin, including fibroblasts and vascular mural cells. Although their biological importance is undisputed, the level of heterogeneity within between organs, while appreciated, has not been analyzed detail. Here, we compare single-cell transcriptional profiles cells across four murine muscular organs: heart, skeletal muscle, intestine bladder. We reveal gene expression signatures that demarcate from provide molecular...
Notch signaling is frequently hyperactivated in breast cancer, but how the enhanced contributes to tumor process less well understood. In this report, we identify proinflammatory cytokine interleukin-6 (IL-6) as a novel target cells. Enhanced upregulated IL-6 expression, leading activation of autocrine and paracrine Janus kinase/signal transducers activators transcription signaling. upregulation was mediated by non-canonical signaling, it could be effectuated cytoplasmically localized...
Alagille syndrome is a genetic disorder characterized by cholestasis, ocular abnormalities, characteristic facial features, heart defects, and vertebral malformations. Most cases are associated with mutations in JAGGED1 (JAG1), which encodes Notch ligand, although it not clear how these contribute to disease development. We aimed develop mouse model of elucidate mechanisms.Mice missense mutation (H268Q) Jag1 (Jag1+/Ndr mice) were outbred C3H/C57bl6 background generate for (Jag1Ndr/Ndr mice)....
Hypoxia limits wound healing. Both normobaric (1 atm) and hyperbaric oxygen (HBO) approaches have been used clinically to oxygenate tissue. Recently, we reported that HBO ameliorates stress-induced impairment of dermal We examined the effect pressure on oxygen-induced vascular endothelial growth factor (VEGF) expression by human HaCaT keratinocytes. Next, investigated whole-body redox ratio oxidized reduced glutathione (GSSG/GSH) in liver, heart, lung, brain rats. Superoxygenation (90% O2)...
Decoding transcriptional effects of experimental tissue–tissue or cell–cell interactions is important; for example, to better understand tumor–stroma after transplantation human cells into mouse (xenografting). Transcriptome analysis intermixed and has, however, frequently relied on the need separate two cell populations prior transcriptome analysis, which introduces confounding gene expression. To circumvent this problem, we here describe a bioinformatics-based, genome-wide technique,...
Hyperactivated Notch signalling has been implicated in breast cancer, but how elevated levels of contribute to mammary dysplasia and tumorigenesis is not fully understood. In this study, we express an activated form Notch1 the mouse luminal lineage analyse consequences for tumour formation transcriptomic landscape lineage. Simultaneous conditional activation a intracellular domain (Notch1 ICD) EGFP was achieved by removal stop cassette CRE-recombinase expression from whey acidic protein...
Abstract N imotuzumab, an anti‐epidermal growth factor receptor (anti‐ EGFR ) monoclonal antibody, has been used extensively in many solid tumors and confers significant survival advantage. The antibody limited skin toxicity is generally well tolerated. Similar to other anti‐ therapies, patients may relapse a few months after treatment. In this study we show for the first time, use of imotuzumab along with S irolimus synergistic effect on tumor inhibition as compared drugs individually,...
Introduction: We still rely on clinical diagnosis for initiating empirical antibiotic therapy and await blood culture confirmation of infection, species identification drug sensitivity. Differential cell count (WBC neutrophils) recording toxic granules in the neutrophils are established methods indirect infection though they not used sepsis per se. Serum Procalcitonin is considered as a good biomarker management sepsis. Materials Methods: Whole serum were laboratory analysis. have combined...
Abstract Homologous recombination deficiency (HRD) arises when defects in DNA repair pathways occur, leading to genomic instability. HRD status is an emerging therapeutic biomarker; NGS assays that measure it can be used stratify ovarian and breast cancer patients determine eligibility for clinical trials, PARP inhibitor- platinum-based therapies. Seraseq FFPE reference materials were developed cover a range of instability scores (GIS) help assay validation development. Here we discuss...