A5102728991- Neurogenesis and neuroplasticity mechanisms
- Pluripotent Stem Cells Research
- Neuroinflammation and Neurodegeneration Mechanisms
- Glioma Diagnosis and Treatment
- RNA Research and Splicing
- Virus-based gene therapy research
- CRISPR and Genetic Engineering
- Liver physiology and pathology
- Neuroscience and Neuropharmacology Research
- Gene Regulatory Network Analysis
- Advanced Fluorescence Microscopy Techniques
- Cardiac Ischemia and Reperfusion
- Birth, Development, and Health
- MicroRNA in disease regulation
- Epigenetics and DNA Methylation
- Single-cell and spatial transcriptomics
- Cell Image Analysis Techniques
- Axon Guidance and Neuronal Signaling
- Genomics and Chromatin Dynamics
- Nerve injury and regeneration
- RNA Interference and Gene Delivery
- Metabolomics and Mass Spectrometry Studies
- Congenital heart defects research
- Microfluidic and Bio-sensing Technologies
- Immune cells in cancer
Yale University
2023-2025
University of South Florida
2016
Madison Group (United States)
2011
Ludwig Cancer Research
1989-2007
Mirus Bio (United States)
2004-2007
University of Rochester
2005-2006
University of Rochester Medical Center
2001-2006
Huntsman Cancer Institute
1996-2003
University of Utah
1996-2003
Western University
2000
Proton nuclear magnetic resonance (1H NMR) spectroscopy is a noninvasive technique that can provide information on wide range of metabolites. Marked abnormalities 1H NMR brain spectra have been reported in patients with neurological disorders, but their neurochemical implications may be difficult to appreciate because data are obtained from heterogeneous tissue regions composed several cell populations. The purpose this study was examine the profile major neural types. This helpful...
We have found that glial progenitor cells isolated from the optic nerves of adult rats are fundamentally different their counterparts in perinatal animals. In our studies on bipotential oligodendrocyte-type-2 astrocyte (O-2A) cells, we seen O-2Aadult can be distinguished O-2Aperinatal progenitors by morphology and antigenic phenotype, much longer cell cycle time (65 h versus 18 h), slower rate migration (4 microns h-1 21 h-1), course differentiation into oligodendrocytes or type-2 astrocytes...
Conserved genomic sequences disrupted in humans may underlie uniquely human phenotypic traits. We identified and characterized 10,032 human-specific conserved deletions (hCONDELs). These short (average 2.56 base pairs) are enriched for brain functions across genetic, epigenomic, transcriptomic datasets. Using massively parallel reporter assays six cell types, we discovered 800 hCONDELs conferring significant differences regulatory activity, half of which enhance rather than disrupt function....
We have isolated a tripotential glial precursor cell population from spinal cords of E13.5 rats. In vitro, these A2B5 + E-NCAM − glial-restricted (GRP) cells can undergo extensive self-renewal, and differentiate into oligodendrocytes two distinct astrocyte populations, but do not neurons. The differentiation potential GRP is retained through at least three cycles expansion recloning. Unlike oligodendrocyte-type 2 progenitor cells, freshly respond to platelet-derived growth factor as mitogen...
ABSTRACT We have found that CNTF and LIF are pleiotropic modulators of development in the O-2A lineage. Both molecules enhanced generation oligodendrocytes cultures dividing progenitors. also promoted oligodendrocyte maturation, as determined by expression myelin basic protein, could promote survival to an extent comparable with insulin-like growth factor-1 or insulin. In addition, both differentiation progenitors into type-2 astrocytes but only when applied presence extra-cellular matrix...
We have shown previously that oligodendrocyte-type-2 astrocyte (O-2A) progenitor cells isolated from adult rat optic nerves can be distinguished in vitro their perinatal counterparts on the basis of much slower rates division, differentiation, and migration when grown presence cortical astrocytes or PDGF. This behavior is consistent with vivo observations there only a modest production oligodendrocytes CNS. As such inconsistent likely need for rapid generation following demyelinating damage...
Delivery is increasingly being recognized as the critical hurdle holding back tremendous promise of nucleic acid-based therapies that include gene therapy and more recently siRNA-based therapeutics. While numerous candidate genes (and siRNA sequences) with therapeutic potential have been identified, their utility has not yet realized because inefficient and/or unsafe delivery technologies. We now describe an intravascular, nonviral methodology enables efficient repeatable acids to muscle...
Changes in gene regulation have been linked to the expansion of human cerebral cortex and neurodevelopmental disorders, potentially by altering neural progenitor proliferation. However, effects genetic variation within regulatory elements on progenitors remain obscure. We use sgRNA-Cas9 screens stem cells (hNSCs) disrupt 10,674 genes 26,385 conserved regions 2,227 enhancers active developing determine Genes with proliferation phenotypes are associated disorders show biased expression...
We have been studying the differing characteristics of oligodendrocyte-type-2 astrocyte (O-2A) progenitors isolated from optic nerves perinatal and adult rats. These two cell types display striking differences in their vitro phenotypes. In addition, O-2Aperinatal progenitor population appears to a limited life-span vivo, while O-2Aadult appear be maintained throughout life. seem largely disappeared nerve by 1 mo after birth, are not detectable cultures derived contrast, can first 7-d-old...
The central nervous system of individuals with multiple sclerosis contains lesions specifically characterized by breakdown myelin sheaths associated a general failure repair demyelinating damage. cause is unknown. Although immune mechanisms have been implicated in this breakdown, no convincing demonstrations specific reaction against yet provided patients. Similarly, the cellular biological which underlie are We found that (i) oligodendrocytes, cells produce system, and (ii)...
Abstract Cell culture techniques, high‐resolution in vitro 1 H NMR spectroscopy, and chromatographic analyses were used to compare the properties of three types human brain nervous system tumours. lines immunocytochemically characterized at all stages with specific antibodies. Intracellular metabolites present cell extracts analysed by spectroscopy high performance liquid chromatography (HPLC). The spectra from meningiomas, neuroblastomas, glioblastomas displayed, addition similarities —...
Anemia frequently accompanies chronic diseases such as progressive renal failure, acquired immunodeficiency syndrome, and cancer. Patients are currently treated with erythropoietin (EPO) replacement therapy, using various recombinant human EPO protein formulations. Although this treatment is effective, gene therapy could be more economical convenient for the long-term management of disease. The objective study was to develop a naked DNA-based protocol that fill need. Hydrodynamic limb vein...
Abstract Human Accelerated Regions (HARs) are highly conserved across species but exhibit a significant excess of human-specific sequence changes, suggesting they may have gained novel functions in human evolution. HARs include transcriptional enhancers with activity and been implicated the evolution brain. However, our understanding how contributed to uniquely features brain is hindered by lack insight into genes pathways that regulate. It unclear whether acted altering expression gene...