A5035732934- Effects of Environmental Stressors on Livestock
- Vector-borne infectious diseases
- Veterinary Oncology Research
- Vector-Borne Animal Diseases
- Microbial Inactivation Methods
- Nanocomposite Films for Food Packaging
- Venomous Animal Envenomation and Studies
- Toxin Mechanisms and Immunotoxins
- Insect and Pesticide Research
- Anesthesia and Sedative Agents
- Veterinary Equine Medical Research
- Veterinary Medicine and Surgery
- Helminth infection and control
- Essential Oils and Antimicrobial Activity
- Food Allergy and Anaphylaxis Research
- Research on Leishmaniasis Studies
- Reproductive Physiology in Livestock
- Animal health and immunology
- Rabies epidemiology and control
- Viral Infections and Vectors
- Meat and Animal Product Quality
- Ruminant Nutrition and Digestive Physiology
- Parasitic Infections and Diagnostics
- Veterinary Pharmacology and Anesthesia
- Microencapsulation and Drying Processes
Universidade Federal de Campina Grande
2014-2024
Universidade Federal da Paraíba
2024
ORCID
2022
Marymount University
2022
Universidade Federal de Pernambuco
2009-2015
Internet Society
2011
A novel lectin was isolated from Bothrops leucurus snake venom using a combination of affinity and gel filtration chromatographies. The (BlL) agglutinated glutaraldehyde-treated rabbit human erythrocytes with preference for erythrocytes. Galactose, raffinose, lactose, fetal bovine serum casein inhibited lectin-induced erythrocyte agglutination. BlL, molecular mass 30 kDa composed two subunits 15 kDa, showed dependence on calcium. BlL is an acidic protein highest activity over the pH range...
Phthirusa pyrifolia leaf lectin (PpyLL) was obtained from the hemi-parasitic medicinal plant mistletoe through saline saturation and two consecutive chromatographic steps on Sephadex G-100 ion exchange CM-cellulose. SDS-PAGE of protein under non-reducing conditions revealed a monomeric with molecular weight 15.6 kDa. Under reducing in presence 2-mercaptoethanol, showed bands weights 15 7 PpyLL, an acidic glycoprotein 19% sugar content, not dependent divalent cations. It stable up to 70 °C...
Neoplastic transformation is the abnormal proliferation of cells. These transformations are often related to changes in cell surface glycoconjugates which can be detected by lectins. We evaluated anti-tumor potential BlL, a galactoside-binding lectin isolated from Bothrops leucurus venom as well its cytotoxicity and hemolysis activity. The phosphatidylserine externalization mitochondrial membrane were also determined. BlL exhibited cytotoxic activity against all tumor lines tested induced...
Objetivo: Caracterizar o perfil epidemiológico dos casos de AIDS na cidade Belém, Pará, Brasil, no período 2019-2023. Métodos :Um estudo descritivo, com abordagem quantitativa, baseado análise 2019-2023.A pesquisa foi realizada utilizando dados secundários disponíveis Sistema Informação Agravos Notificação (SINAN). Resultados: Com base nos apresentados, formou-se um que revela os indivíduos do sexo masculino, faixa etária 20 a 34 anos, da raça parda, escolaridade ensino médio e se...
Avaliou-se o efeito de diferentes temperaturas sobre os parâmetros fisiológicos, bioquímicos e hormonais ovinos Soinga, Morada Nova, Santa Inês SRD, em câmara climática. Foram utilizados 24 machos não castrados, baias contendo 8 animais cada (2 grupo genético), expostos a 3 ambientes térmicos (AT = 28 °C, 32 °C 36 °C) por um período 15 dias, 10 horas dia. mensuradas temperatura retal (TR), frequência respiratória (FR) superficial (TS) avaliados (Cortisol, T3 T4) após exposição temperatura. O...
Objetivou-se avaliar a capacidade adaptativa dos genótipos Soinga e Santa Inês, submetidos diferentes dietas ambientes térmicos, por meio de parâmetros fisiológicos hematológicos. O experimento utilizou com silagem sorgo concentrado (50:50 70:30). Foram registradas variáveis ambientais, como temperatura ambiente, umidade relativa globo negro, calculado o índice do negro umidade. Avaliaram-se retal (TR), superficial (TS), frequência respiratória (FR), glicose sanguínea As coletas ocorreram às...
Objetivou-se avaliar e comparar a adaptabilidade dos genótipos Soinga Santa Inês em função da dieta do ambiente térmico. Foram utilizados 24 ovinos machos, com média de 150 dias idade peso inicial 20,00 ± 3,00 kg, distribuídos grupos alimentados silagem sorgo concentrado nas proporções volumoso:concentrado 50:50 70:30. Avaliaram-se variáveis ambientais como temperatura ambiente, umidade relativa globo negro, cálculo ITGU carga térmica radiação (CTR). Os parâmetros fisiológicos analisados...
A lectin from Sebastiania jacobinensis bark was isolated using a combination of acetone precipitation, ammonium sulphate fractionation, ion exchange and gel filtration chromatographies. The purified, with molecular mass 52.0 kDa composed two subunits 24 kDa, is glycoprotein neutral carbohydrate content 6.94%. shows maximum activity over the pH range 4.0–7.5 heat stability up to 70 °C. Our results show that an incompetitive inhibitor for trypsin, Ki 0.39 ± 0.02 μM. Fluorescence spectroscopy...
WGA, an agglutinin from wheat germ which is largely responsible for many of wheat's allergies, was used as a model to investigate the action ionising radiation on WGA's anti-nutritive effects in sensitised mice. Based molecular structure, present study also examined structural modification WGA relation range dose. Structural integrity monitored using HPLC, fluorescence spectrometry and circular dichroism. Results showed loss intrinsic activity formation insoluble amorphous aggregates with...
Summary Serodiagnosis of visceral leishmaniasis is often hindered by cross‐reactions to other parasitic diseases. Identifying specific B ‐cell epitopes in proteins therefore important for immunodiagnostics, as well disease control vaccines. This study aimed identify linear and conformational evaluate the secondary structure antigen Leishmania infantum using silico analysis. Linear were predicted Immune Epitope Database Analysis Resource ( IEDB ), BepiPred BcePred programs. The identified...
Food irradiation is the process of exposing food to ionising radiation eliminate microorganisms or insects. Lectins are most common agents in intolerance, and efficient methods reduce unwanted intolerant immunological effects lectins have not yet been described. Sebastiania jacobinensis bark lectin was structurally altered after gamma irradiation. Hemagglutination assays showed that stimulated by low doses (0.1 kGy), while high (above 1 kGy) induced a significant loss activity. The effect...
This research evaluated the effects of energy supplementation on sheep’s feeding behavior, feed preference, and thermoregulatory responses using technical cashew nutshell liquid (CNSL) different vegetable oils with unsaturated fatty acid (UFA) compositions. The experiment was completely randomized five treatments: a mixture CNSL (0.5%) + [canola (high in monounsaturated acids—MUFA), corn, soybean, sunflower, or cottonseed oil polyunsaturated acids-PUFA) at 1.5%] based total diet dry matter,...
Crataeva tapia bark lectin was evaluated for its antitumor activity against sarcoma 180 in Swiss albino mice. The anti-inflammatory and analgesic properties were investigated models of inflammation nociception. assay induced by carrageenan peritonitis the acetic acid-induced writhing response. presents low toxicity with a LD50 2,500 mg/kg body weight significant causing inhibition tumor growth. also promoted reduction (35.4%) number neutrophil migration carrageenan. Concerning property,...
Phytochemical analyses of four different extract (water, butanol, ethyl ether and acetate) dried leaves Phthirusa pyrifolia their antioxidant potential (2,2-diphenyl-2-picrylhydrazyl hydrate radical-scavenging, reducing power hydroxyl radical scavenging activity) were measured. Results showed that the highest lowest contents components including phenols, flavonoids condensed tannins found in water acetate extracts, respectively. Furthermore, exhibited better capacities than other samples...
In ruminant feeding, mechanisms for controlling the rate of ammonia release in rumen are important increasing efficiency transforming dietary nitrogen into microbial protein. Three microencapsulated formulations, with increased urea concentrations 10 (MPec1), 20 (MPec2) and 30% (MPec3) from w/w, based on mass citrus pectin solution, employ external ionic gelation/extrusion technique. The properties were examined as a completely randomized design 5 treatments each replicates evaluation,...
Few chronic food protein models have described the relationship between allergenicity and molecular structure of after physical processing. The effect γ-radiation on was measured by fluorescence, circular dichroism microcalorimetry. BALB/c mice were intraperitoneally sensitized then given non-irradiated irradiated Con-A daily gavage for 28days. tendency to form insoluble amorphous aggregates partially unfolded species observed irradiation. administration samples at low-dose significantly...