A5101818350- SARS-CoV-2 detection and testing
- Mesenchymal stem cell research
- Biosensors and Analytical Detection
- interferon and immune responses
- Advanced Chemical Sensor Technologies
- COVID-19 diagnosis using AI
- Immune Cell Function and Interaction
- Immunotherapy and Immune Responses
- Bacteriophages and microbial interactions
- Tissue Engineering and Regenerative Medicine
- HIV Research and Treatment
- T-cell and B-cell Immunology
- Advanced biosensing and bioanalysis techniques
- SARS-CoV-2 and COVID-19 Research
- Extracellular vesicles in disease
- Cytokine Signaling Pathways and Interactions
- vaccines and immunoinformatics approaches
- Bone and Joint Diseases
- Cancer Cells and Metastasis
- melanin and skin pigmentation
- Corneal Surgery and Treatments
- Protein Kinase Regulation and GTPase Signaling
- Melanoma and MAPK Pathways
- Reproductive System and Pregnancy
- HIV/AIDS drug development and treatment
National Jewish Health
2019-2024
University of Colorado Denver
2019-2024
University of Colorado Anschutz Medical Campus
2019-2024
Swedish University of Agricultural Sciences
2021
Pontificia Universidad Católica de Chile
2020
Millennium Institute for Integrative Biology
2020
University of Buenos Aires
2020
University of Colorado Health
2020
University of Iowa
2016-2019
Denver School of Nursing
2019
The adaptive immune system is a natural diagnostic and therapeutic. It recognizes threats earlier than clinical symptoms manifest neutralizes antigen with exquisite specificity. Recognition specificity broad reactivity enabled via B- T-cell receptors: the receptor repertoire. human system, however, not omnipotent. Our defense sometimes loses battle to parasites microbes even turns against us in case of cancer, autoimmune inflammatory disease. A long-standing dream immunoengineers has been,...
Abstract The ability to use mesenchymal stromal cells (MSC) directly out of cryostorage would significantly reduce the logistics MSC therapy by allowing on-site therapeutic doses at hospitals and clinics. Such a paradigm be especially advantageous for treatment acute conditions such as stroke myocardial infarction, which are likely require within hours after ischemic onset. Recently, several reports have emerged that suggest viability potency damaged cryopreservation. Herein we examine...
The use of mesenchymal stromal cell (MSC) therapy for the treatment type 2 diabetes (T2D) and T2D complications is promising; however, investigation MSC function in setting has not been thoroughly explored. In our current study, we investigated phenotype MSCs a simulated vitro environment. We show that palmitate, but glucose, exposure impairs metabolic activity with moderate increases apoptosis, while drastically affecting proliferation morphology. co-culture peripheral blood mononuclear...
The COVID-19 pandemic has resulted in millions of deaths globally, and while several diagnostic systems were proposed, real-time reverse transcription polymerase chain reaction (RT-PCR) remains the gold standard. However, reagents, including enzymes used RT-PCR, are subject to centralized production models intellectual property restrictions, which present a challenge for less developed countries. With aim generating standardized One-Step open RT-qPCR protocol detect SARS-CoV-2 RNA clinical...
ABSTRACT From the first isolation of Zika virus (ZIKV) in Uganda 1947, ZIKV had primarily been associated with sporadic human cases Africa and Asia until emerged as an epidemic Americas 2015. As spread into new geographic regions, it now has potential to interact many novel host species whose susceptibility yet be determined. Mathematical models predict a sylvatic reservoir ZIKV, non-human primates (NHPs) likely candidates, though outbreaks areas without NHPs suggest presence other...
Purpose: The purpose of this study was to determine mesenchymal stem cell (MSC) therapy efficacy on rescuing the visual system in experimental autoimmune encephalomyelitis (EAE) model multiple sclerosis (MS) and provide new mechanistic insights. Methods: EAE induced female C57BL6 mice by immunization with myelin oligodendrocyte glycoprotein (MOG)35–55, complete Freund's adjuvant, pertussis toxin. findings were compared sham-immunized mice. Half received intraperitoneally delivered cells (EAE...
αβ T cell receptor (TCR) V(D)J genes code for billions of TCR combinations. However, only some appear on peripheral cells in any individual because, to mature, thymocytes must react with low affinity but not high thymus expressed major histocompatibility (MHC)/peptides. MHC proteins are very polymorphic. Different alleles bind different peptides. Therefore, might express many ensure that peptides from an invader bound and activate cells. most individuals limited numbers alleles. To explore...
Reverse transcription-loop-mediated isothermal amplification (RT-LAMP) has gained popularity for the detection of severe acute respiratory syndrome coronavirus 2 (SARS-CoV-2). The high specificity, sensitivity, simple protocols, and potential to deliver results without use expensive equipment made it an attractive alternative RT-PCR. However, cost per reaction, centralized manufacturing required reagents, their distribution under cold chain shipping limit RT-LAMP's applicability in...
Abstract Melanoma represents the most serious type of skin cancer. Although recent years have seen advances using targeted and immunotherapies, patients remain at high risk for tumor recurrence. Here we show that IRAK-M, a negative regulator MyD88 signaling, is deficient or low in melanoma expression levels correlate with patient survival. Inducing IRAK-M genetic approaches epigenetic modifiers initiates apoptosis by prompting its interaction TRAF6 via IRAK-M’s C-terminal domain. This...
ABSTRACT RT-LAMP (reverse transcription - Loop-mediated isothermal amplification) has gained popularity for the detection of SARS-CoV-2. The high specificity, sensitivity, simple protocols and potential to deliver results without use expensive equipment made it an attractive alternative RT-PCR. However, cost per reaction, centralized manufacturing required reagents their distribution under cold chain shipping limits RT-LAMP’s applicability in low-income settings. preparation assays using...
This protocol has been optimized for the recombinant expression of a codon-optimized Bst-LF polymerase. The goal this was to eliminate use large volumes dyalisis through concentrators buffer exchange before storage conditions. plasmid encoding enzyme used here can be found on reclone.org
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Although single layer centrifugation (SLC) selects robust spermatozoa from stallion semen, the effect of individual variation has not been studied in detail. The objective this study was to determine among stallions effects SLC on sperm quality during cooled storage for up 48 hr. Semen samples seven (18 ejaculates) were split, with one portion being used and other serving as a control (CON). Sperm (kinematics, reactive oxygen species (ROS) production, membrane integrity (MI) chromatin...
About: This protocol has been optimized for recombinant expression of molony virus based reverse transcriptases (RT). The plasmid used contains a transcriptase gene which is derived from Moloney Murine Leukemia Virus. enzyme when expressed recombinantly can synthesizes complementary DNA strand single-stranded RNA, DNA, or an RNA:DNA hybrid. Enzyme Engineering: point mutations to generate highly processive, thermostable, and improved fidelity mutant transcriptase. Details, incluiding the...
Objective: Techniques to validate successful delivery of cell products are expensive, time-consuming, and require transport the animal imaging facilities, preventing their widespread use as documentation tools. The goal this study was determine if a low-cost portable microscope could provide sufficient performance be used document track retention over time. Approach: A Dino-Lite fluorescence an Odyssey CLx whole-animal scanner were compared on basis resolution, sensitivity, linearity. impact...
The COVID-19 pandemic has resulted in millions of deaths globally, and while several diagnostic systems were proposed, real-time reverse transcription polymerase chain reaction (RT-PCR) remains the gold standard. However, reagents, including enzymes used RT-PCR, are subject to centralized production models intellectual property restrictions, which present a challenge for less developed countries. With aim generating standardized One-Step open RT-qPCR protocol detect SARS-CoV-2 RNA clinical...
Abstract Human mesenchymal stromal cells (MSCs) are a leading cell therapy candidate for the treatment of immune and inflammatory diseases due to their potent regulation cells. MSC expression indoleamine-2,3-dioxygenase (IDO) upon interferon gamma exposure has been proposed as both sentinel marker key mediator immunomodulatory potency. Rather than wait in vivo cytokines, MSCs can be pre-licensed during manufacturing enhance IDO expression. In this study, we systematically examine relative...