A5022007083- Neuroscience and Neuropharmacology Research
- SARS-CoV-2 and COVID-19 Research
- Aquatic Ecosystems and Phytoplankton Dynamics
- Monoclonal and Polyclonal Antibodies Research
- Nicotinic Acetylcholine Receptors Study
- Receptor Mechanisms and Signaling
- Nitric Oxide and Endothelin Effects
- Marine and coastal ecosystems
- bioluminescence and chemiluminescence research
- Biocrusts and Microbial Ecology
- vaccines and immunoinformatics approaches
- Neuroendocrine regulation and behavior
- Nanoplatforms for cancer theranostics
- Medicinal Plants and Neuroprotection
- Anaerobic Digestion and Biogas Production
- Advanced Proteomics Techniques and Applications
- Protist diversity and phylogeny
- CRISPR and Genetic Engineering
- Advanced biosensing and bioanalysis techniques
- Biochemical effects in animals
- Bacteriophages and microbial interactions
- GABA and Rice Research
- Wastewater Treatment and Reuse
- Gene Regulatory Network Analysis
- Environmental Toxicology and Ecotoxicology
Fundación Ciencias Exactas y Naturales
2017-2025
University of Buenos Aires
2014-2025
Consejo Nacional de Investigaciones Científicas y Técnicas
2012-2025
Millennium Institute for Integrative Biology
2020
Pontificia Universidad Católica de Chile
2020
National Agricultural Technology Institute
2016-2018
Abstract The yeast Pichia pastoris is a cost-effective and easily scalable system for recombinant protein production. In this work we compared the conformation of receptor binding domain (RBD) from severe acute respiratory syndrome coronavirus-2 (SARS-CoV-2) Spike expressed in P. well established HEK-293T mammalian cell system. RBD obtained both cells was properly folded, as indicated by UV-absorption, circular dichroism tryptophan fluorescence. They also had similar stability,...
Abstract This paper reports the design of a biosensor for sensitive, low-cost measurement lead in drinking water. The uses genetically-modified strain Escherichia coli , which serves as both signal amplifier and reporter water, measured via colour change. We developed PlomBOX platform to image this change we demonstrate its capability detect concentrations low World Health Organisation upper limit water 10 ppb. Our approach does not require expensive infrastructure or expert operators,...
The receptor binding domain (RBD) of the Spike protein from SARS-CoV-2 is a promising candidate to develop effective COVID-19 vaccines since it can induce potent neutralizing antibodies. We have previously reported highly efficient production RBD in Pichia pastoris, which structurally similar same produced mammalian HEK-293T cells. In this work we designed an multimer with purpose increasing its immunogenicity. multimeric particles by transpeptidation reaction between expressed P. pastoris...
Abstract The yeast Pichia pastoris is a cost-effective and easily scalable system for recombinant protein production. In this work we compared the conformation of receptor binding domain (RBD) from SARS-CoV-2 Spike expressed in P. well established HEK-293T mammalian cell system. RBD obtained both cells was properly folded, as indicated by UV-absorption, circular dichroism tryptophan fluorescence. They also had similar stability, temperature-induced unfolding (observed T m were 50 °C 52...
Abstract The diversity and flexibility of life offers a wide variety molecules systems useful for biosensing. A biosensor device should be robust, specific reliable. Inorganic arsenic is highly toxic water contaminant with worldwide distribution that poses threat to public health. With the goal developing an biosensor, we designed incoherent feed-forward loop (I-FFL) genetic circuit correlate its output pulse input signal in relatively time-independent manner. system was conceived...
BACKGROUND AND PURPOSE NO is a highly diffusible and reactive gas produced in the nervous system, which acts as neuronal signal mediating physiological or pathological mechanisms. can modulate activity of neurotransmitter receptors ion channels, including NMDA GABA A receptors. In present work, we examined whether C receptor function also be regulated by NO. EXPERIMENTAL APPROACH Homomeric ρ1 were expressed oocytes GABA‐evoked responses electrophysiologically recorded presence absence donor...
Reactive oxygen species (ROS) are normally involved in cell oxidative stress but also play a role as cellular messengers redox signalling; for example, modulating the activity of neurotransmitter receptors and ion channels. However, direct actions ROS on GABAA were not previously demonstrated. In present work, we studied effects ρ1 receptor function.GABAA expressed oocytes GABA-evoked responses electrophysiologically recorded presence or absence ROS. Chemical protection cysteines by...
This protocol has been optimized for the recombinant expression of a codon-optimized Bst-LF polymerase. The goal this was to eliminate use large volumes dyalisis through concentrators buffer exchange before storage conditions. plasmid encoding enzyme used here can be found on reclone.org
Abstract Since the discovery of SARS-CoV-2, several antigens have been proposed to be part COVID-19 vaccines. The receptor binding domain (RBD) Spike protein is one promising candidates develop effective vaccines since it can induce potent neutralizing antibodies. We previously reported production RBD in Pichia pastoris and showed structurally identical produced mammalian HEK-293T cells. In this work we designed an multimer construct with purpose increasing immunogenicity. multimeric...
Abstract The presence of cyanobacterial toxins in freshwater constitutes an increasing public health concern, especially affecting developing countries where the high cost available methods makes monitoring programs difficult. phosphatase inhibition assay (PPIAs) is a sensitive method with low instrument requirements that allows quantification most frequent cyanotoxins, microcystins (MC). In this work, we implemented PPIAs, starting from Protein Phosphatase 1 (PP1) expression up to...