A5108053930- X-ray Diffraction in Crystallography
- Crystallization and Solubility Studies
- Bacterial Genetics and Biotechnology
- Enzyme Structure and Function
- Crystallography and molecular interactions
- Plant Reproductive Biology
- Bacterial biofilms and quorum sensing
- Drug Transport and Resistance Mechanisms
- Estrogen and related hormone effects
- Plant Molecular Biology Research
- Photoreceptor and optogenetics research
- Hepatitis B Virus Studies
- RNA and protein synthesis mechanisms
- Protist diversity and phylogeny
- Cell death mechanisms and regulation
- Plant-Microbe Interactions and Immunity
- Bacteriophages and microbial interactions
- Plant responses to water stress
- Microbial Metabolites in Food Biotechnology
- Plant Parasitism and Resistance
- Amino Acid Enzymes and Metabolism
- Plant tissue culture and regeneration
- Immune Response and Inflammation
- Polysaccharides and Plant Cell Walls
- Advanced Breast Cancer Therapies
University of Exeter
2018-2024
University of Lisbon
2005-2018
Newcastle University
2010-2013
Durham University
2005-2007
University of Minnesota Medical Center
1999
Regulation of neutrophil activation is critical for disease control. Neutrophil extracellular traps (NETs), which are web-like structures composed DNA and neutrophil-derived proteins, formed following pro-inflammatory signals; however, if this process uncontrolled, NETs contribute to pathogenesis, exacerbating inflammation host tissue damage
The control of mRNA stability is an important component regulation in bacteria. Processing and degradation mRNAs are initiated by endonucleolytic attack, the cleavage products processively degraded exoribonucleases. In many bacteria, these RNases, as well RNA helicases other proteins, organized a protein complex called degradosome. Escherichia coli, degradosome assembled around essential endoribonuclease E. Bacillus subtilis, recently discovered RNase Y involved initiation degradation....
Bacillus subtilis mutants lacking ymdB are unable to form biofilms, exhibit a strong overexpression of the flagellin gene hag, and deficient in SlrR, SinR antagonist. Here, we report functional structural characterization YmdB, find that YmdB is phosphodiesterase with activity against 2′,3′- 3′,5′-cyclic nucleotide monophosphates. The structure reveals enzyme adopts conserved fold binuclear metal center. Mutagenesis catalytically crucial residue demonstrates enzymatic essential for biofilm...
Maintenance of F-actin turnover is essential for plant cell morphogenesis. Actin-binding protein mutants reveal that plants place emphasis on particular aspects actin biochemistry distinct from animals and fungi. Here we show in CAP1, an A. thaliana member the cyclase-associated family, display a phenotype establishes CAP1 as fundamental facilitator dynamics over wide range tissues. Plants homozygous cap1 alleles reduction stature morphogenetic disruption multiple types. Pollen grains...
Highlights•FORMIN4 is delivered to sites of cell wall reinforcement during immune responses•Delivery requires actin filaments and causes local enhancement dynamics•Genetic disruption FORMIN4 close homologs a defense phenotype•Transport localization distinct from key protein PEN3SummaryCell appositions (CWAs) are produced reactively by the plant system arrest microbial invasion through inversion growth. This process controlled invagination plasma membrane (PM) in coordination with export...
Summary The stomatal complex of grasses consists two guard cells and adjacent subsidiary that cooperate during closure. Zymoseptoria tritici , the main causal agent Septoria blotch in wheat, enters host via stomata. Here we test hypothesis shows focused sub-cellular responses to invading Z. hyphae. We have combined live-cell transmission light microscopy, immunofluorescence CRS microscopy identify cell wall modifications triggered by hyphal invasion. Furthermore, used confocal fluorescence...
Abstract Chirality is ubiquitous in nature, with consequences at the cellular and tissue scales. As Escherichia coli colonies expand radially, an orthogonal component of growth creates a pinwheel-like pattern that can be revealed by fluorescent markers. To elucidate mechanistic basis this colony chirality, we investigated its link to left-handed, single-cell twisting during E. elongation. While chemical genetic manipulation cell width altered handedness, ceased chiral rather than switching...
Chirality is ubiquitous in nature, with consequences at the cellular and tissue scales. As Escherichia coli colonies expand radially, an orthogonal component of growth creates a pinwheel-like pattern that can be revealed by fluorescent markers. To elucidate mechanistic basis this colony chirality, we investigated its link to left-handed, single-cell twisting during E. elongation. While chemical genetic manipulation cell width altered handedness, ceased chiral rather than switching anaerobic...