A5013186889- Porphyrin Metabolism and Disorders
- RNA and protein synthesis mechanisms
- Folate and B Vitamins Research
- Cellular transport and secretion
- RNA modifications and cancer
- Bacterial Genetics and Biotechnology
- Innovative Teaching Methods
- Photosynthetic Processes and Mechanisms
- Protein Kinase Regulation and GTPase Signaling
- Biochemical and Molecular Research
- ATP Synthase and ATPases Research
- CRISPR and Genetic Engineering
- Fungal and yeast genetics research
- Microtubule and mitosis dynamics
- RNA Research and Splicing
- Online and Blended Learning
- Iron Metabolism and Disorders
- Advanced Electron Microscopy Techniques and Applications
- Experimental Learning in Engineering
- Polyamine Metabolism and Applications
- 14-3-3 protein interactions
- Bacteriophages and microbial interactions
- Heme Oxygenase-1 and Carbon Monoxide
- Genetics, Bioinformatics, and Biomedical Research
- Bioinformatics and Genomic Networks
University of California, Berkeley
2014-2023
University of Wisconsin–Madison
2017
Massachusetts Institute of Technology
2012
Cornell University
2012
Much evidence shows that instruction actively engages students with learning materials is more effective than traditional, lecture-centric instruction. These "active learning" models comprise an extremely heterogeneous set of instructional methods: they often include collaborative activities, flipped classrooms, or a combination both. To date, it unclear whether active if combines collaboration support classroom methods. We conducted quasi-experiment as part advanced general chemistry course...
Protein kinases have evolved diverse specificities to enable cellular information processing. To gain insight into the mechanisms underlying kinase diversification, we studied CMGC protein using ancestral reconstruction. Within this group, cyclin dependent (CDKs) and mitogen activated (MAPKs) require proline at +1 position of their substrates, while Ime2 prefers arginine. The resurrected common ancestor CDKs, MAPKs, could phosphorylate substrates with or arginine, preference for proline....
Some bacterial mRNAs contain a region called riboswitch which controls gene expression by binding to metabolite in the cell. Typically, riboswitches sense and respond limited range of cellular metabolites, often just one type.
The "flavin destructase" enzyme BluB catalyzes the unprecedented conversion of flavin mononucleotide (FMN) to 5,6-dimethylbenzimidazole (DMB), a component vitamin B(12). Because its unusual chemistry, mechanism this transformation has remained elusive. This study reports identification 12 mutant forms that have severely reduced catalytic function, though most retain ability bind flavin. is an fragments cofactor FMNH(2) in presence oxygen produce lower axial ligand B(12) (cobalamin). Despite...
Summary (Abstract) Macromolecular crowding has a profound impact on reaction rates and the physical properties of cell interior, but mechanisms that regulate are poorly understood. We developed Genetically Encoded Multimeric nanoparticles (GEMs) to dissect these mechanisms. GEMs homomultimeric scaffolds fused fluorescent protein. self-assemble into bright, stable particles defined size shape. By combining tracking with genetic pharmacological approaches, we discovered mTORC1 pathway can tune...
The mechanisms underlying the evolution of phosphoregulatory networks are poorly understood. We sought to understand kinase specificity using CMGC (Cdk1, MAPK, Gsk, CK) family as a model. This has undergone dramatic functional diversification and therefore represents useful model for evolution. have reconstructed ancestral sequences entire tree maximum likelihood method. synthesized, purified profiled specificities common ancestor family; several ancestors leading Cdk1 paralog, Ime2. Our...
The ability to sense and respond intracellular metabolite levels enables cells adapt environmental conditions. Many prokaryotes use riboswitches - structured RNA elements usually located in the 5' untranslated region of mRNAs metabolites by modulating gene expression. corrinoid riboswitch class, which responds adenosylcobalamin (coenzyme B12) related metabolites, is among most widespread bacteria. structural for binding requirement a kissing loop interaction between aptamer expression...
In addition to proteins, microbes can use structured RNAs such as riboswitches for the important task of regulating gene expression. Riboswitches control expression by changing their structure in response binding a small molecule and are widespread among bacteria. Here we determine mechanism regulation riboswitch that responds corrinoids-a family coenzymes related vitamin B12. We report alternative RNA secondary structures couple corrinoid sensing with repressing novel activating riboswitch....
Abstract In bacteria, many essential metabolic processes are controlled by riboswitches, gene regulatory RNAs that directly bind and detect metabolites. Highly specific effector binding enables riboswitches to respond a single biologically relevant metabolite. Cobalamin potential exception because over dozen chemically similar but functionally distinct cobalamin variants (corrinoid cofactors) exist in nature. Here, we measured riboswitch activity vivo using Bacillus subtilis fluorescent...