A5038213822- Protein purification and stability
- Enzyme Structure and Function
- Protein Structure and Dynamics
- Monoclonal and Polyclonal Antibodies Research
- Viral Infectious Diseases and Gene Expression in Insects
- Microfluidic and Bio-sensing Technologies
- Microfluidic and Capillary Electrophoresis Applications
- Chemical Synthesis and Analysis
- Diatoms and Algae Research
- Blood properties and coagulation
- Molecular Junctions and Nanostructures
- Rheology and Fluid Dynamics Studies
- Spectroscopy and Quantum Chemical Studies
- Nanopore and Nanochannel Transport Studies
- Proteins in Food Systems
- Food Waste Reduction and Sustainability
- Advanced biosensing and bioanalysis techniques
Lund University
2020-2024
Stevens Institute of Technology
2020
University of Rome Tor Vergata
2017
Charges and their contribution to protein–protein interactions are essential for the key structural dynamic properties of monoclonal antibody (mAb) solutions. In fact, they influence apparent molecular weight, static structure factor, collective diffusion coefficient, or relative viscosity, concentration dependence. Further, charges play an important role in colloidal stability mAbs. There exist standard experimental tools characterize mAb net charges, such as measurement electrophoretic...
Developing efficient and robust computational models is essential to improve our understanding of protein solution behavior. This becomes particularly important tackle the high-concentration regime. In this context, main challenge put forward coarse-grained descriptions able reduce level detail, while retaining key features relevant information. work, we develop an strategy that can be used investigate gain insight into monoclonal antibody solutions under different conditions. We use a...
We present a scheme for transferring conformational degrees of freedom from all-atom (AA) simulations an intrinsically disordered protein (IDP) to coarse-grained (CG) Monte Carlo (MC) using swap moves. AA single histatin 5 peptide in water were used obtain structural ensemble, which is reweighted CGMC simulation the presence negatively charged surface. For efficient sampling, trajectory was condensed two approaches: RMSD clustering (based on root-mean-square difference atom positions) and...
The interface between biological matter and inorganic materials is a widely investigated research topic due to possible applications in biomedicine nanotechnology. In this context, the molecular level adsorption mechanism that drives specific recognition small peptide sequences surfaces represents an important likely provide much information useful for designing bioderived materials. Here, we investigate dynamics at Ti-binding sequence (AMRKLPDAPGMHC) TiO2 anatase surface by using (MD)...
Interactions between biomolecules are ubiquitous in nature and crucial to many applications including vaccine development; environmentally friendly textile detergents; food formulation. Using small angle X-ray scattering structure-based molecular simulations, we explore protein-protein interactions dilute semi-concentrated protein solutions. We address the pertinent question, whether interaction models developed at infinite dilution can be extrapolated concentrated regimes? Our analysis is...
Using light scattering (LS), small-angle X-ray (SAXS), and coarse-grained Monte Carlo (MC) simulations, we studied the self-interactions of two monoclonal antibodies (mAbs), PPI03 PPI13. With LS measurements, obtained osmotic second virial coefficient, B22, molecular weight, Mw, mAbs, while with SAXS mAbs' self-interaction behavior in high protein concentration regime up to 125 g/L. Through SAXS-derived representations performed MC simulations either a one-protein or two-protein model...
Aggregation is a common phenomenon in the field of protein therapeutics and can lead to function loss or immunogenic patient responses. Two strategies are currently used reduce aggregation: (1) finding suitable formulation, which labor-intensive requires large quantities, (2) engineering protein, extensive knowledge about aggregation pathway. We present biophysical characterization oligomerization processes by Interferon alpha-2a (IFNα-2a), drug with antiviral immunomodulatory properties....
The goal of the paper is to design and implement a system measure food waste in Pierce Dining Hall on campus Stevens Institute Technology. would likely utilize camera identify types based database containing several varieties food. Some important variables consider are amount made initially, after disposal (potentially determined through weight, density, etc.), taken from serving line. These data values can be analyzed compared promote efficiency production minimization future. To achieve...
Charges and their contribution to protein-protein interactions are essential for the key structural dynamic properties of monoclonal antibody (mAb) solutions. In fact, they influence apparent molecular weight, static structure factor, collective diffusion coefficient or relative viscosity concentration dependence. Further, charges play an important role in colloidal stability mAbs. There exist standard experimental tools characterise mAb net such as measurement electrophoretic mobility,...
The osmotic pressure of dilute electrolyte solutions containing charged macro-ions as well counterions can be computed directly from the particle distribution via well-known cell model. Originally derived within Poisson-Boltzmann mean-field approximation, model considers a single macro-ion centered into cell, together with needed to neutralize total charge, while it neglects phenomena due correlations. While extensively applied in coarse-grained Monte Carlo (MC) simulations continuum solvent...
Therapeutic proteins can be challenging to develop due their complexity and the requirement of an acceptable formulation ensure patient safety efficacy. To date, there is no universal development strategy that identify optimal conditions for all types in a fast reliable manner. In this work, high-throughput characterization, employing toolbox five techniques, was performed on 14 structurally different formulated 6 buffer presence 4 excipients. Multivariate data analysis chemometrics were...
Aggregation and oligomer formation are critical parameters in the field of protein therapeutics can lead to loss drug function or even immunogenic responses patients. Currently two approaches used reduce aggregation: (1) finding a suitable formulation which is labor-intensive requires large quantities (2) engineering by specific, stabilizing mutations, specific knowledge about aggregation pathway. We present biophysical characterization oligomerization process Interferon alpha-2a, with...
Aggregation and oligomer formation are critical parameters in the field of protein therapeutics can lead to loss drug function or even immunogenic responses patients. Currently two approaches used reduce aggregation: (1) finding a suitable formulation which is labor-intensive requires large quantities (2) engineering by specific, stabilizing mutations, specific knowledge about aggregation pathway. We present biophysical characterization oligomerization process Interferon alpha-2a, with...