A5086461965- Identification and Quantification in Food
- Meat and Animal Product Quality
- Environmental DNA in Biodiversity Studies
- Molecular Biology Techniques and Applications
- Web Data Mining and Analysis
- Experimental Learning in Engineering
- Metaheuristic Optimization Algorithms Research
- Milk Quality and Mastitis in Dairy Cows
- Advanced Chemical Sensor Technologies
- Food Allergy and Anaphylaxis Research
- Urban Transport and Accessibility
- Probiotics and Fermented Foods
- Monoclonal and Polyclonal Antibodies Research
- Fuzzy Logic and Control Systems
- Imbalanced Data Classification Techniques
- Advanced Multi-Objective Optimization Algorithms
- Service-Oriented Architecture and Web Services
- Escherichia coli research studies
- Water Quality Monitoring Technologies
- Ichthyology and Marine Biology
- Vehicle Routing Optimization Methods
- Anomaly Detection Techniques and Applications
- Genetic diversity and population structure
- E-commerce and Technology Innovations
- Biosensors and Analytical Detection
Consejo Superior de Investigaciones Científicas
2024
Universidad de Granada
2002-2024
Universidad de Huelva
2011-2023
Universidad Complutense de Madrid
2003-2013
<title>Abstract</title> <bold>Background/aims</bold> It is unclear whether or how low- and middle-income countries have implemented Advance Care Planning (ACP) Directives (AD). We aimed to map the current state of ACP/AD in Latin America. <bold>Methods</bold> This cross-sectional mixed-methods survey LA comprised interviews with 18 key informants from out 20 countries, most whom were appointed by national Palliative Associations. Interviews occurred online each informant encompassing various...
A TaqMan real-time PCR method based on nucleotide sequence variation in the D-loop and 12S rRNA mitochondrial genes has been developed for specific detection of chicken, turkey, duck, goose prohibited material animal feeds. The assay uses 4 primer/probe sets targeting short species-specific sequences together with a positive amplification control eukaryotic 18S gene. applicability was assessed through analysis batch industrial feed samples subjected to different rendering temperatures...
Within the field of linguistic fuzzy modeling with rule-based systems, automatic derivation rules from numerical data is an important task. In last few years, a large number contributions based on techniques such as neural networks and genetic algorithms have been proposed to face this problem. article, we introduce novel approach rule learning problem ant colony optimization (ACO) algorithms. To do so, task formulated combinatorial Our process COR methodology in previous works, which...
PCR method was applied for the qualitative identification of chicken (Gallus gallus), turkey (Meleagris gallipavo), duck (Anas platyrhynchos × Cairina muschata), and goose (Anser anser) tissues in feed-stuffs, on an individual basis. The assay uses oligonucleotide primers that are specific each avian species, targeting 12S rRNA mitochondrial gene. designed generated amplicons 95, 122, 64, 98 bp length chicken, turkey, duck, goose, respectively. specificity tested against 29 animal species...
ABSTRACT Raw and smoked Atlantic salmon ( Salmo salar ) rainbow trout Oncorhynchus mykiss were differentiated by PCR amplification of a 950 bp conserved fragment the mitochondrial 16S rRNA gene followed restriction site analysis with endonucleases Hpa I Bst Ell. Salmon products digested yielded two fragments 804 146 bp, while not cleaved this enzyme. However, Ell did cleave bands 513 437 produced when samples This genetic marker could be very useful for detecting fraudulent substitution...
ABSTRACT Restriction site analysis of the internal transcribed spacer (ITS) region amplified by polymerase chain reaction (PCR) was used for specific identification 3 clam species: Ruditapes decussatus (grooved carpet shell), Venerupis pullastra (pullet and philippinarum (Japanese shell). PCR amplification using primers based on nucleotide sequences Mytilus ITS regions produced a fragment 1195 bp in R. , 1074 V. 1188 inR. philippinarum. Digestion products with endonucleases HinfI Rsa I...
Journal Article A quantitative PCR‐ELISA for the rapid enumeration of bacteria in refrigerated raw milk Get access R. Gutiérrez, Gutiérrez Departamento de Nutrición y Bromatología III, Facultad Veterinaria,Universidad Complutense, Madrid, Spain Search other works by this author on: Oxford Academic Google Scholar T. García, García I. González, González B. Sanz, Sanz P.E. Hernández, Hernández Martín Dr Rosario 28040‐Madrid, (e‐mail : rmartin@eucmax.sim.ucm.es). Applied Microbiology, Volume 83,...
Journal Article Rapid enumeration of Escherichia coli in oysters by a quantitative PCR‐ELISA Get access I. González, González Departamento de Nutrición y Bromatologia III, Facultad Veterinaria, Universidad Complutense, Madrid, Spain Search for other works this author on: Oxford Academic Google Scholar T. García, García A. Fernández, Fernández B. Sanz, Sanz P. E. Hernández, Hernández R. Martín Applied Microbiology, Volume 86, Issue 2, 1 February 1999, Pages 231–236,...
A PCR assay was developed for the identification of meats and commercial meat products from quail (Coturnix coturnix), pheasant (Phasianus colchicus), partridge (Alectoris spp.), guinea fowl (Numida meleagris), pigeon (Columba Eurasian woodcock (Scolopax rusticola), song thrush (Turdus philomelos) based on oligonucleotide primers targeting specific sequences mitochondrial D-loop region. The designed generated fragments 96, 100, 104, 106, 147, 127, 154 bp in length quail, pheasant, partridge,...
Polymerase chain reaction-RFLP analysis has been applied to the identification of meats from quail (Coturnix coturnix), pheasant (Phasianus colchicus), red-legged partridge (Alectoris rufa), chukar chukar), guinea fowl (Numida meleagris), capercaillie (Tetrao urogallus), Eurasian woodcock (Scolopax rusticola), and woodpigeon (Columba palumbus). reaction amplification was carried out using a set primers flanking conserved region approximately 310 bp mitochondrial D-loop region. Restriction...
A TaqMan real-time PCR method was developed for specific detection of porcine-prohibited material in industrial feeds. The assay combines the use a porcine-specific primer pair, which amplifies 79 bp fragment mitochondrial (mt) 12 S rRNA gene, and locked nucleic acid (LNA) probe complementary to target sequence lying between primers. nuclear 18 gene system, yielding 77 amplicon, employed as positive amplification control monitor total content amplifiable DNA samples. specificity porcine...
Two PCR assays for the identification of partridge meat (red-legged partridge, chukar barbary and gray species) specific red-legged products were developed based on species-specific primers targeting 12S ribosomal RNA mitochondrial gene. Moreover, various techniques use random amplified polymorphic DNA markers nuclear growth hormone rhodopsin genes tested to find a method differentiation between pure hybrid partridges. Among these techniques, amplification sequencing gene fragment was...
A polymerase chain reaction (PCR) based on oligonucleotide primers targeting the mitochondrial 12S rRNA gene was developed for specific identification of rabbit DNA (Oryctolagus cuniculus) in food and feedstuffs. The specificity verified by PCR analysis from 32 non-target species including mammals, birds, fish, plant species. Analysis experimental mixtures demonstrated presence rabbit-derived materials range 0.1-100%. Prolonged heat treatment (up to 133ºC 20 min at 300 kPa) applied...