A5079392873- RNA Interference and Gene Delivery
- Plant biochemistry and biosynthesis
- Biochemical Acid Research Studies
- Microbial Metabolic Engineering and Bioproduction
- Organic Chemistry Cycloaddition Reactions
- Nanoparticle-Based Drug Delivery
- Metabolomics and Mass Spectrometry Studies
- Metabolism and Genetic Disorders
- Advanced Nanomaterials in Catalysis
- Lipid Membrane Structure and Behavior
- Sphingolipid Metabolism and Signaling
- Supramolecular Self-Assembly in Materials
University of Tennessee at Knoxville
2022-2024
Liposomes are effective therapeutic delivery nanocarriers due to their ability encapsulate and enhance the pharmacokinetic properties of a wide range therapeutics. Two primary areas in which improvement is needed for liposomal drug infiltrate cells facilitate derivatization liposome surface. Herein, we report platform incorporating cyclic disulfide lipid (CDL) dual purpose enhancing cell entry functionalizing membrane through thiol-disulfide exchange. In order accomplish this, CDL-1 CDL-2,...
We report boronate-caged guanidine-lipid 1 that activates liposomes for cellular delivery only upon uncaging of this compound by reactive oxygen species (ROS) to produce cationic lipid products. These are designed mimic the exceptional cell properties cell-penetrating peptides (CPPs), while inclusion boronate cage is enhance selectivity such entry will be activated in presence ROS. Boronate hydrogen peroxide was verified mass spectrometry and zeta potential (ZP) measurements. A...
Bacterial isoprenoids are necessary for many biological processes, including maintaining membrane integrity, facilitating intercellular communication, and preventing oxidative damage. All bacterial biosynthesized from two five carbon structural isomers, isopentenyl pyrophosphate (IPP) dimethylallyl (DMAPP), which cell impermeant. Herein, we demonstrate exogenous delivery of IPP DMAPP into Bacillus subtilis by utilizing a self-immolative ester (SIE)-caging approach. We initially evaluated...
Abstract Isopentenyl pyrophosphate (IPP) and dimethylallyl (DMAPP) are the central five‐carbon precursors to all terpenes. Despite their significance, exogenous, independent delivery of IPP DMAPP cells is impossible as negatively charged makes these molecules membrane impermeant. Herein, we demonstrate a facile method circumvent this challenge through esterification β‐phosphate with two self‐immolative esters (SIEs) that neutralize yield membrane‐permeant analogs DMAPP. Following cellular...
Multifunctional: Cyclic disulfide liposomes have been developed for the dual purposes of enhancing cellular delivery through reaction with cell-surface thiols and facilitating liposome functionalization. These are depicted in form beach balls being lofted at their target cells: a scene warm day summertime issue Chemistry—A European Journal. More information can be found Research Article by M. D. Best co-workers (DOI: 10.1002/chem.202201164).