A5083543238- Advanced Proteomics Techniques and Applications
- Electrospun Nanofibers in Biomedical Applications
- Nerve injury and regeneration
- Prostate Cancer Treatment and Research
- Pluripotent Stem Cells Research
- Mass Spectrometry Techniques and Applications
- Spectroscopy and Chemometric Analyses
- 3D Printing in Biomedical Research
- Molecular Biology Techniques and Applications
- Nerve Injury and Rehabilitation
- CRISPR and Genetic Engineering
- Bone Tissue Engineering Materials
- Spectroscopy Techniques in Biomedical and Chemical Research
- Ubiquitin and proteasome pathways
- Gallbladder and Bile Duct Disorders
- Cancer, Lipids, and Metabolism
- Microfluidic and Bio-sensing Technologies
- Copyright and Intellectual Property
- Animal Genetics and Reproduction
- Tissue Engineering and Regenerative Medicine
- Peptidase Inhibition and Analysis
- Case Reports on Hematomas
- Cancer Research and Treatments
- Gastrointestinal Bleeding Diagnosis and Treatment
- Bee Products Chemical Analysis
University of Sheffield
2008-2023
Cardiff University
2013-2018
Royal Hallamshire Hospital
2008-2009
The peripheral nervous system has a limited innate capacity for self-repair following injury, and surgical intervention is often required. For injuries greater than few millimeters autografting standard practice although it associated with donor site morbidity in its availability. Because of this, nerve guidance conduits (NGCs) can be viewed as an advantageous alternative, but currently have efficacy short large injury gaps comparison to autograft. Current commercially available NGC designs...
The unpredictable behavior of prostate cancer presents a major clinical challenge during patient management. In order to gain an insight into the molecular mechanisms associated with progression, we employed shot-gun proteomic approach isobaric tags for relative and absolute quantitation (iTRAQ), followed by 2D-LC-MS/MS, using poorly metastatic LNCaP cell line its highly variant LNCaP-LN3 as model. A total number 280 unique proteins were identified (≥95% confidence), expression data was...
In this work, we report a method to acquire and analyze hyperspectral coherent anti-Stokes Raman scattering (CARS) microscopy images of organic materials biological samples resulting in an unbiased quantitative chemical analysis. The employs singular value decomposition on the square root CARS intensity, providing automatic determination components above noise, which are retained. Complex susceptibility spectra, linear composition, retrieved from intensity spectra using causality by two...
A major challenge in the management of patients with prostate cancer is identifying those individuals at risk developing metastatic disease, as most cases disease will remain indolent. We analyzed pooled serum samples from 4 groups (n = 5 samples/group), collected prospectively and actively monitored for a minimum yrs. Patients were (i) histological diagnosis benign prostatic hyperplasia no evidence 'BPH', (ii) localised progression, 'non-progressing' (iii) biochemical 'progressing', (iv)...
The use of nerve guidance conduits (NGCs) to treat peripheral injuries is a favorable approach the current "gold standard" autografting. However, as simple hollow tubes, they lack specific topographical and mechanical cues present in grafts therefore are not suitable for treating large gap (30–50 mm). incorporation intraluminal scaffolds, such aligned fibers, has been shown increase neuronal cell neurite outgrowth Schwann migration distances. A novel blend PHAs, P(3HO)/P(3HB) (50:50), was...
Abstract BACKGROUND Due to the heterogeneity in biological behavior of prostate cancer, biomarkers that can reliably distinguish indolent from aggressive disease are urgently needed inform treatment choices. METHODS We employed 8‐plex isobaric Tags for Relative and Absolute Quantitation (iTRAQ), profile proteomes two distinct panels isogenic cancer cells with varying growth metastatic potentials, order identify novel associated progression. The LNCaP, LNCaP‐Pro5, LNCaP‐LN3 panel represent a...
Peripheral nerves are basic communication structures guiding motor and sensory information from the central nervous system to receptor units. Severed peripheral nerve injuries represent a large clinical problem with relevant challenges successful synthetic repair scaffolds as substitutes autologous grafting. Numerous studies reported use of hollow tubes made polymers sutured between severed stumps promote regeneration while providing protection for external factors, such scar tissue...
Autografts are the current gold standard for large peripheral nerve defects in clinics despite frequently occurring side effects like donor site morbidity. Hollow guidance conduits (NGC) proposed alternatives to autografts, but failed bridge gaps exceeding 3 cm humans. Internal NGC cues microfibres believed enhance hollow NGCs by giving additional physical support directed regeneration of Schwann cells and axons. In this study, we report a new 3D vitro model that allows evaluation different...
Severe peripheral nerve injuries represent a large clinical problem with relevant challenges such as the development of successful synthetic scaffolds substitutes to autologous grafting. Numerous studies have reported use polyesters and type I collagen-based guidance conduits (NGCs) promote regeneration through critical defects while providing protection from external factors. However, none commercially available hollow bioresorbable NGCs demonstrated superior outcomes an graft. Hence, new...
A major challenge in the management of patients with prostate cancer is identifying those individuals at risk developing metastatic disease, as most cases disease will remain indolent.We analyzed pooled serum samples from 4 groups (n = 5 samples/group), collected prospectively and actively monitored for a minimum yrs.Patients were (i) histological diagnosis benign prostatic hyperplasia no evidence 'BPH', (ii) localised progression, 'non-progressing' (iii) biochemical 'progressing', (iv) bone...
Prostate cancer (PCa) is the most common diagnosis and second cause of cancer-related deaths in men. Currently, serum prostate-specific antigen (PSA) only biomarker widely used management patients with PCa. However, PSA lacks diagnostic sensitivity specificity, leading to false-negative false-positive test results. cannot distinguish indolent from aggressive disease, many being over-treated associated side-effects. Furthermore, unable identify which tumors are likely become unresponsive...
Stem cells have received much attention recently for their potential utility in regenerative medicine. The identification of differentiated progeny often requires complex staining procedures, and is challenging intermediary stages which are a priori unknown. In this work, the ability label‐free quantitative coherent anti‐Stokes Raman scattering (CARS) micro‐spectroscopy to identify populations intermediate cell states during differentiation murine embryonic stem into adipocytes assessed....
Producing induced pluripotent stem cells (iPSCs) from human tissue for use in personalized medicine strategies or therapeutic testing is at the forefront of medicine. Therefore, identifying a source to reprogram that easily accessible via simple noninvasive procedure great clinical importance. Reprogramming these iPSCs through nonintegrating methods genetic manipulation paramount regenerative purposes. Here, we demonstrate reprogramming oral mucosal lamina propria progenitor patients...