Sarcophagid flies have many characteristics that make them ideal forensic indicators. However, their utility is severely limited because it difficult or impossible to determine the species of a sarcophagid larva, and in instances an adult specimen, based on anatomy. We developed database mitochondrial DNA sequence data makes possible identify all likely be found feeding human corpse at urban location Canada USA. Analyses were 783 base pair region gene for cytochrome oxidase subunit one...

The time elapsed since death, or postmortem interval (PMI), is a matter of crucial importance in investigations homicides and other untimely deaths. Such information can help to identify both the criminal victim by eliminating suspects connecting deceased with individuals reported missing for same amount (Catts 1990, Geberth 1996). Even when cause death natural, have important implications legal matters such as inheritance insurance (Henssge et al. 1995). Crucial information, was last seen...

Mitochondrial DNA was used to infer the phylogeny and genetic divergences of Chrysomya albiceps (Wiedemann) C. rufifacies (Maquart) specimens from widely separated localities in Old New World. Analyses based on a 2.3-kb region including genes for cytochrome oxidase subunits I II indicated that 2 species were separate monophyletic lineages have been > 1 million years. Analysis DNA, form either sequence or restriction fragment-length polymorphism (RFLP) data, will permit identification...

Abstract During the course of our forensic investigations, we have encountered situations where it would been useful to evidence, other than direct contact between two, for concluding that a carrion-fly maggot developed on particular human victim. If collected during death investigation did not develop victim, then its age is relevant estimating postmortem interval. In this study demonstrate mitochondrial DNA (mtDNA) sequence data can be obtained from dissected gut had fed tissue. These used...

Abstract Four Old World blow flies (Diptera: Calliphoridae), Chrysomya albiceps (Wiedemann), C. putoria Wiedemann, megacephala (Fabricius), and rufifacies (Macquart), have recently invaded the New World. The interaction of (Macquart) with native carrion in Texas, USA, was investigated by reducing oviposition invader on rabbit carcasses outdoors. These produced significantly more Cochliomyia macellaria (Fabricius) adults compared to which not reduced. results suggest that populations will...

The development of the Oriental latrine fly, Chrysomya megacephala (Fabricius), was examined at 27℃ in order to resolve discrepancies previous reports. Various developmental events were completed by all larvae following ages : egg hatch, 18hr; first molt, 30hr; second 72hr; pupariation, 144hr; adult emergence, 234hr. Differing descriptions C. appear reflect variation length postfeeding period, during which time may be particularly sensitive environmental conditions. By sampling entire age...

Approximately 8% of calyptrate species diversity comes from the Calliphoridae, which includes flies medical, veterinary, and forensic importance. The status family Calliphoridae has for years been central systematic problem superfamily Oestroidea, phylogenetic relationships between key groups are unresolved controversial. We reconstructed phylogenies within larger context other Oestroidea based on 5,189 bp combined data one mitochondrial (cytochrome oxidase subunit one) three nuclear...

Journal Article Forensic Use of Megaselia abdita and M. scalaris (Phoridae: Diptera): Case Studies, Development Rates, Egg Structure Get access Bernard Greenberg, Greenberg 1Department Biological Sciences, University Illinois at Chicago, IL 60607 Search for other works by this author on: Oxford Academic PubMed Google Scholar Jeffrey D. Wells 2Division Insect Biology, Department ESPM, California, Berkeley, CA 94720-3112 Medical Entomology, Volume 35, Issue 3, 1 May 1998, Pages 205–209,...

Entomological evidence is most often used for estimating the postmortem interval, but fly larvae can also be a source of vertebrate DNA. Forensic analysis DNA recovered from larva's gut to identify what larva had been feeding on. During our previous research studies, we same extraction dual purpose identifying insect species and associating maggot with its last meal. In experience, have encountered several situations where this method corpse would useful, such as removal remains suspected...

Journal Article Effect of the Red Imported Fire Ant (Hymenoptera: Formicidae) and Carcass Type on Daily Occurrence Postfeeding Carrion-Fly Larvae (Diptera: Calliphoridae, Sarcophagidae) Get access Jeffrey D. Wells, Wells 1 Department Biological Sciences, University Illinois at Chicago, IL 60680 1Current address: Entomology, Louisiana State Agricultural Center, Baton Rouge, LA 70803. Search for other works by this author on: Oxford Academic PubMed Google Scholar Bernard Greenberg Medical...

Journal Article Commonly Used Intercarcass Distances Appear to Be Sufficient Ensure Independence of Carrion Insect Succession Pattern Get access A. E. Perez, Perez 2 1 Department Biology, Saint Joseph's College, 1498 S College Ave., Rensselaer, IN 47978 ( aperez2@saintjoe.edu ; blowfly@technologist.com ), Corresponding author, e-mail: Search for other works by this author on: Oxford Academic Google Scholar N. H. Haskell, Haskell J. D. Wells 3 Biological Sciences and International Forensic...

Several authors claimed that a forensic entomological analysis can never be interpreted as the postmortem interval (PMI), but it minimum (PMImin ), or dead for not less than X amount of time. Because in most instances, PMI estimate should range, is, set values from maximum (PMImax ) to PMImin , objections estimation are PMImax estimation. The arguments this position did address substantial body literature describing both and using insect succession analysis. This report reviews scientific...

The introduction of 4 Chrysomya Robineau-Desvoidy spp. to the Americas has made obsolete previously published keys Nearctic calliphorid larvae, particularly those covering subfamily Chrysomyinae. To assist forensic entomologists, ecologists, and public health workers, we provide a key 3rd instars 8 chrysomyine species reported from or likely occur in carrion within continental United States. rare (in States) Cochliomyia aldrichi Del Ponte, C. minima Shannon, Chloroprocta idioidea...

Timetables of carrion-arthropod succession provide critical baseline data for calculating entomology-based estimates the postmortem interval (PMI) in cases natural and untimely death; however, statistical confidence intervals typically do not accompany such because lack methodology. Using 2 computer-intensive sampled randomization tests (the Jackknife Bootstrap) from 3 studies succession, we investigated degree to which PMI width (upper limit - lower + 1) was affected by missing taxa, corpse...