The recently developed iCELLigence™ real‑time cell analyzer (RTCA) can be used for the label‑free monitoring of cancer proliferation, viability, invasion and cytotoxicity. RTCA system uses 16‑well microtiter plates with a gold microelectrode biosensor array that measures impedance when cells adhere to microelectrodes causing an alternating current. By measuring electric field generated in this process, analysis morphology migration. present review aimed summarize working method system,...

The regeneration of bone tissues using a composite-based tissue engineering approach is promising strategy for repairing and restoring their biological functions. This study extensively investigates the development potential applications Multi-Walled Carbon Nanotube-Polylactic Acid (MWCNT-PLA) composites defect healing. scaffolds were meticulously engineered characterized to assess material properties, structural compatibility, biodegradability, pH impact, water absorption, biocompatibility,...

The interaction between diphtheria toxin (DT) and nicotinamide adenine dinucleotide (NAD) is central to DT's enzymatic activity, which involves ADP-ribosylation of eukaryotic elongation factors. This study aims elucidate how solvent environments influence the thermodynamic structural properties DT-NAD interaction. Using Raman Spectroscopy, complementary techniques, we analyzed two different DTs, by using Differential Scanning Calorimetry (DSC) try understand binding under varying conditions,...

The degree of monomer conversion (DC) values three different dental composites were examined using methods: surface microhardness (ratio bottom/top), Fourier-transform infrared (FT-IR), and differential scanning calorimetry (DSC). Two the included in study nanohybrid (Dentsply Neo Spectra ST HV Omnichroma), one was a microhybrid-labeled newly marketed composite containing nanoparticles (Dentac Myra). Composite discs prepared according to methodology for all methods analyzed (2 mm thickness ×...

Rapid and easy detection of pathogens, especially bacteria, plays an important role in daily life the face increasing environmental pollution. Nanosensors focus on carbon nanotubes, which can bind various molecules to large surfaces have high electron conductivity. They are widely used development electrochemical biosensors. In this study, a new design biosensors is presented by integrating gold-coated tungsten wires (GC-TW) acid-functionalized multi-walled nanotubes (MWCNTs) two-step...

Carbon nanotubes have become widely used due to their superior physiochemical properties, and so knowledge of toxicity has important. In this study, the toxic effects on Caenorhabditis elegans been investigated. Multi-walled carbon were characterized by transmission electron microscopy particle size analysis. Upon exposure at concentrations 100 500 mg/L for 3 15 days, numbers body lengths nematodes not altered, but thickness was raised movements slightly decreased. A new type...

Carbon nanotubes (CNT) have high surface area and absorption capabilities the capacity of multi-walled CNTs (MWNTs) enables paramagnetic to be obtained by absorbing iron salts. Characterization MWNTs was performed with TEM, FTIR, DSC, VSM. Paramagnetic are used for purification water from heavy metals plastic originated compounds. Most PVC-based materials contain phthalate acid esters (PAE) which considerable effects on human health. In this study, were removing diethyl (DEP) methanol after...

ABSTRACT Optogenetics, a revolutionary technique leveraging light stimulation for precise cellular control, holds immense potential in regenerative medicine. Offering unparalleled spatial and temporal accuracy, the entrance of optogenetics into tissue engineering medicine (TERM) empowers researchers to modify genes precisely reversibly, control signal pathways antibodies, as well alter biomaterial properties. Optogenetics provides unprecedented manipulation physiological regeneration,...

Abstract The Differential Scanning Calorimeter (DSC) technique is commonly used for quantitative analysis of protein stability, and it one the methods to quantify protein-ligand interactions. DSC provides denaturation curves that can be a source data on binding constants. B-cell leukemia/lymphoma-2 (BCL-2) an antiapoptotic protein, overexpressed proteins in cancer cells. Here, constants BCL-2 nine inhibitors were evaluated by utilization measurements. free energies suggested measured melting...

AFs are among the most dangerous compounds, exhibiting carcinogenic effects on human and liver tissue. Due to their high toxicity established limits in food feed products, there is a growing demand for more accurate sensitive detection methods. Detecting of paramount importance ensuring safety, as even low concentrations can pose risks both humans livestock. Various techniques such electrochemical immunosensors, ELISA tests, chromatographic spectroscopic methods extensively employed quantify...

Poly ADP-ribose polymerases (PARPs) are family of proteins that use nicotinamide adenine dinucleotide (NAD) as substrate. Seventeen putative PARP sequences were determined in the human genome. Although PARPs show a variety functions and low sequence identities, they share common structural functional properties. In our study, PARP1 PARP2 PARP4 different species compared; it was found active sites for human, rat mouse have highly conserved sequence. Overall folding PARP1, confirms similarity...

Amaç: Grafen yapının iç içe silindirler oluşturması ile elde edilen çok duvarlı karbon nanotüpler (ÇDNT), geniş yüzey alanları, hidrofobik yapıları, yüksek elektron iletkenlikleri kullanım alanı bulmaktadırlar. İlaç taşıma özellikleri değerlendirilmekle birlikte, biyosensörlerde ve polimerlerle birlikte antibakteriyel malzeme sentezlenmesinde kullanılmaktadırlar. Farklı fizikokimyasal özelliklere sahip ÇDNT’lerin toksisitelerine dair sayıda veri vardır. Bunların yanında son yıllarda...

Sinirbilim, milyonlarca nöron ve milyarlarca sinapstan oluşan insan beyninin yapı fonksiyonlarını anlamaya çalışır. Laboratuvar ortamında böylesine gelişmiş bir sistem için model oluşturmak incelemek mümkün değildir. Yirmi yılı aşkın süredir, sinir sistemine benzer özellikleri kolay incelenebilir yapısı nedeniyle Caernohabditis elegans (C. elegans), nöral ağların davranışını anlamak faydalı olarak öne çıkmaktadır. Oluşturulan modeller, davranış aktivitelerin nicel analizlerinin yapılmasını,...

Tea and coffee are the most popular beverage there is a variety of forms in both hot cold temperatures. Black tea made from green leaves that have been oxidized. During oxidation process, certain proportion polyphenol compounds lost. More than thousand chemical components can be found coffee, making it complex beverage. One significant factors stressed equally to scent flavor caffeine content. Differential Scanning Calorimetry (DSC) was employed our study compare properties Turkish black...

Milk proteins occupy a prominent place in the nutrition of adults and children. Generally, some commercial dairy contains proteins, lactose, other sugar derivatives, additives. The proportions components that make up milk are different milk. For this reason, analyzing correctly is essential for determining these contents. In research, analyses were made by taking differential scanning calorimetry measurements (DSC), Fourier transform infrared spectrophotometer (FTIR) measurements. Specific...