A5043262342- Plant pathogens and resistance mechanisms
- CRISPR and Genetic Engineering
- Plant tissue culture and regeneration
- Chromosomal and Genetic Variations
- Plant biochemistry and biosynthesis
- Plant Virus Research Studies
- Nitrogen and Sulfur Effects on Brassica
- Genomics, phytochemicals, and oxidative stress
- Photosynthetic Processes and Mechanisms
- Insect Resistance and Genetics
- Lipid metabolism and biosynthesis
- Plant Gene Expression Analysis
- Plant-Microbe Interactions and Immunity
- 14-3-3 protein interactions
- Lignin and Wood Chemistry
Yangzhou University
2018-2024
Targeted genome editing is a desirable means of basic science and crop improvement. The clustered, regularly interspaced, palindromic repeat (CRISPR)/Cas9 (CRISPR-associated 9) system currently the simplest most commonly used in targeted genomic plants. Single multiplex plants can be achieved under this system. In Arabidopsis, AtWRKY11 AtWRKY70 genes were involved JA- SA-induced resistance to pathogens, rapeseed (Brassica napus L.), BnWRKY11 BnWRKY70 found differently expressed after...
Base editor technology that enables precise base-editing has been developed by employing Cas9 nickase (nCas9) or deactivated (dCas9) fused to an enzyme with base conversion activity, named cytidine-deaminase-mediated (CBE, C•G T•A) adenine-deaminase-mediated (ABE, A•T G•C) (Gaudelli et al., 2017; Komor 2016). The systems have successfully applied in several plant species, including Arabidopsis, rice, wheat, maize, tomato and cotton (Mao 2019). However, it is not clear whether editing will...
Allopolyploids require rapid genetic and epigenetic modifications to reconcile two or more sets of divergent genomes. To better understand the fate duplicate genes following genomic mergers doubling during allopolyploid formation, in this study, we explored global gene expression patterns resynthesized allotetraploid Brassica napus (AACC) its diploid parents B. rapa (AA) oleracea (CC) using RNA sequencing leaf transcriptomes. We found that formation was accompanied by extensive changes...
Brassica napus is one of the important oil crops grown worldwide, and quality improvement a major goal in rapeseed breeding. Yellow seed an excellent trait, which has great potential improving economic value. In this study, we created stable yellow mutants using CRISPR/Cas9 system obtained phenotype only when four alleles two BnTT2 homologues were knocked out, indicating that had conserved but redundant functions regulating color. Histochemical staining flavonoid metabolic analysis proved...
Sclerotinia sclerotiorum causes severe yield and economic losses for many crop vegetable species, especially Brassica napus . To date, no immune B. germplasm has been identified, giving rise to a major challenge in the breeding of resistance. In present study, we found that, compared with -susceptible line (J902), -resistant (J964) exhibited better xylem development higher lignin content stems, which may limit invasion spread S. during early infection period. addition, genes involved...
Abstract Sclerotinia stem rot (SSR), caused by the necrotrophic fungus sclerotiorum , is one of most devastating diseases for several major oil-producing crops. Despite its impact, genetic basis SSR resistance in plants remains poorly understood. Here, through a genome-wide association study, we identify key gene, BnaA07. MKK9 that encodes mitogen-activated protein kinase confers oilseed rape. Our functional analyses reveal BnaA07.MKK9 interacts with BnaC03.MPK3 and BnaC03.MPK6...
Sclerotinia sclerotiorum is generally considered one of the most economically damaging pathogens in oilseed rape (Brassica napus). Breeding for resistance challenging, as no immune germplasm available B. napus. It desirable to develop new breeding strategies. In present study, host-induced gene silencing (HIGS), developed based on RNA interference (RNAi), was applied protect napus from S. infection. Three pathogenicity genes, endo-polygalacturonase (SsPG1), cellobiohydrolase (SsCBH), and...
Oilseed rape plants with abscission-defective floral organs acquired through genome editing show less susceptibility to Sclerotinia sclerotiorum infection and longer flowering-period for flower tourism.
Rapeseed (Brassica napus L.) with substantial lipid and oleic acid content is of great interest to rapeseed breeders. Overexpression Glycine max transcription factors Dof4 Dof11 increased accumulation in Arabidopsis microalgae, addition modifying the quantity certain fatty components. Here, we report involvement GmDof4 GmDof11 regulating composition rapeseeds. reduced linoleic linolenic acid. Both qPCR yeast one-hybrid assay indicated that activated expression FAB2 by directly binding...
The G-box regulating factors (GRFs) are involved in a wide array of signal transduction pathway and play important roles plant physiological developmental processes stress responses. GRF proteins have previously been described several species, but not rapeseed (Brassica napus L.). In this study, we carried out genome-wide analysis GRFs B. based on the available genome sequence information, analyzed their expression different tissues under hormone treatments after inoculation with Sclerotinia...