A5078533037- RNA modifications and cancer
- RNA Research and Splicing
- RNA and protein synthesis mechanisms
- HVDC Systems and Fault Protection
- Ubiquitin and proteasome pathways
- Cardiac Structural Anomalies and Repair
- CRISPR and Genetic Engineering
- Receptor Mechanisms and Signaling
- Healthcare Systems and Reforms
- Cardiac Fibrosis and Remodeling
- Ion Channels and Receptors
- Congenital heart defects research
- Cancer-related gene regulation
- Ion channel regulation and function
Heidelberg University
2019-2023
German Centre for Cardiovascular Research
2019-2023
University Hospital Heidelberg
2019-2023
Cornell University
2020
Conceptually similar to modifications of DNA, mRNAs undergo chemical modifications, which can affect their activity, localization, and stability. The most prevalent internal modification in mRNA is the methylation adenosine at N6-position (m6A). This returns a role as central hub information within cell, serving an carrier, modifier, attenuator for many biological processes. Still, precise such m6A human murine-dilated cardiac tissue remains unknown. Transcriptome-wide mapping allowed us...
Abstract RNA–protein interactions are central to cardiac function, but how activity of individual RNA-binding protein is regulated through signaling cascades in cardiomyocytes during heart failure development largely unknown. The mechanistic target rapamycin kinase a hub that controls mRNA translation cardiomyocytes; however, direct link between mTOR and proteins the has not been established. Integrative transcriptome translatome analysis revealed dependent translational upregulation RNA...
m6A mRNA methylation controls cardiomyocyte function and increased overall levels are a stereotyping finding in heart failure independent of the underlying etiology. However, it is largely unknown how information read by reader proteins failure. Here we show that protein Ythdf2 cardiac identified novel mechanism control gene expression function. Deletion cardiomyocytes vivo leads to mild hypertrophy, reduced function, fibrosis during pressure overload as well aging. Similarly, vitro...
RNA-binding proteins (RBPs) control critical aspects of cardiomyocyte function, but the repertoire active RBPs in cardiomyocytes during growth response is largely unknown. We define healthy and diseased at a system-wide level by RNA interactome capture. This identifies 67 cardiomyocyte-specific RBPs, including several contractile proteins. Furthermore, we identify cytoplasmic polyadenylation element-binding protein 4 (Cpeb4) as dynamic RBP, regulating cardiac both vitro vivo. mRNAs bound to...
Article4 October 2021Open Access Source DataTransparent process Muscle-specific Cand2 is translationally upregulated by mTORC1 and promotes adverse cardiac remodeling Agnieszka A Górska orcid.org/0000-0003-4667-6690 Department of Cardiology, Angiology Pneumology, University Hospital Heidelberg, Germany DZHK (German Centre for Cardiovascular Research), partner site, Heidelberg/Mannheim, These authors contributed equally to this work Search more papers author Clara Sandmann...
RNA binding proteins (RBPs) have the potential to affect most post-transcriptional steps in gene expression. RBPs control critical elements of cellular function, but their specific role cardiomyocytes is largely unknown. Here we defined healthy and diseased primary at a system-wide level by Interactome Capture. This identified 67 novel cardiomyocyte including several contractile proteins. Furthermore, Cytoplasmic polyadenylation element protein 4 (Cpeb4) as dynamic mRBP cardiomyocytes,...
In India over 2 lakh new cases of end stage kidney disease (ESRD get added every year but only a minority (10%) are able to some form Renal replacement therapy (RRT). USRDS 2014 hemodialysis (HD) data shows that among dialysis population average hospitalization rate is 1.7/patient/year with mean duration stay 10.9 days/year. There paucity on & mortality in HD from India. We prospectively studied the patients at our center.
Abstract The mechanistic target of rapamycin (mTOR) is a key regulator pathological remodeling in the heart by activating ribosomal biogenesis and mRNA translation. Inhibition mTOR cardiomyocytes protective, however, detailed role translational regulation specific networks diseased largely unknown. A cardiomyocyte genome-wide sequencing approach was used to define mTOR-dependent post-transcriptional gene expression control at level This identified muscle-specific protein Cullin-associated...
Abstract m 6 A mRNA methylation controls cardiomyocyte function and increased overall levels are a stereotyping finding in heart failure independent of the underlying etiology. However, it is largely unknown how information read by reader proteins failure. Here we show that protein Ythdf2 cardiac identified novel mechanism control gene expression function. Deletion cardiomyocytes vivo leads to hypertrophy, reduced function, fibrosis during pressure overload as well aging. Similarly, vitro...