A5088523544- Atomic and Subatomic Physics Research
- Advanced NMR Techniques and Applications
- Advanced MRI Techniques and Applications
- Supramolecular Chemistry and Complexes
- Quantum, superfluid, helium dynamics
- Electron Spin Resonance Studies
- Photoreceptor and optogenetics research
- Lanthanide and Transition Metal Complexes
- Medical Imaging Techniques and Applications
- Astronomy and Astrophysical Research
- Boron Compounds in Chemistry
- Ocean Acidification Effects and Responses
- Chemical Reactions and Isotopes
- Hemoglobin structure and function
- Cancer Genomics and Diagnostics
- Nutrition, Genetics, and Disease
- Ultrasound and Hyperthermia Applications
- Cephalopods and Marine Biology
- Geophysics and Gravity Measurements
- Molecular Spectroscopy and Structure
- Marine Biology and Ecology Research
- Stellar, planetary, and galactic studies
Leibniz-Forschungsinstitut für Molekulare Pharmakologie
2012-2021
Forschungsverbund Berlin
2020
California Institute of Technology
2018
Biosensor (Italy)
2015
Leibniz Institute for Neurobiology
2011-2012
Institut d'Astrophysique de Paris
2011
European Southern Observatory
2011
Vrije Universiteit Brussel
2011
Labile complexation of cucurbit[6]uril with xenon becomes detectable through saturation transfer highly efficient gas turnover for ultra-sensitive NMR applications.
Abstract Caged xenon has great potential in overcoming sensitivity limitations for solution‐state NMR detection of dilute molecules. However, no application such a system as magnetic resonance imaging (MRI) contrast agent yet been performed with live cells. We demonstrate MRI localization cells labeled caged packed‐bed bioreactor working under perfusion hyperpolarized‐xenon‐saturated medium. Xenon hosts enable NMR/MRI experiments switchable and selectivity cell‐associated versus unbound...
The reversible binding of xenon to host molecules has found numerous applications in nuclear magnetic resonance studies. Quantitative characterization the Xe exchange dynamics is important understand and optimize physico-chemical behavior such hosts, but often challenging achieve at low concentrations. We have investigated a sensitive quantification technique based on chemical saturation transfer with hyperpolarized nuclei, qHyper-CEST. Using simulated signals we demonstrated that...
Ultrasound and hyperpolarized magnetic resonance imaging enable the visualization of biological processes in deep tissues. However, few molecular contrast agents are available to connect these modalities specific aspects function. We recently discovered that a unique class gas‐filled protein nanostructures known as gas vesicles could serve nanoscale reporters for modalities. need produce via expression specialized cultures cyanobacteria or haloarchaea limits their broader adoption by other...
Xenon is well known to undergo host-guest interactions with proteins and synthetic molecules. As xenon can also be hyperpolarized by spin exchange optical pumping, allowing the investigation of highly dilute systems, it makes an ideal nuclear magnetic resonance probe for such host The utility as a further improved using Chemical Exchange Saturation Transfer nuclei (Hyper-CEST), but accurate experiments requires polarizer infusion system optimized measurements. We present design...
The NMR signal of hyperpolarized 129Xe trapped in cryptophane cages different solvents experiences chemical shifts. An encoding method is presented that involves the optimal use reversible Xe binding and efficiently uses hyperpolarization. This utilized nanomolar imaging, subsecond time-resolved studies while maintaining high spectral selectivity. Detailed facts importance to specialist readers are published as "Supporting Information". Such documents peer-reviewed, but not copy-edited or...
The combination of hyperpolarized Xe with chemical exchange saturation transfer (Hyper-CEST) is a powerful NMR technique to detect highly dilute concentrations binding sites using RF pulses. Crucially, that pulse strength and duration generates the maximal Hyper-CEST effect priori unknown. In contrast CEST in proton MRI, where system reaches steady-state for long times, has an optimal time, i.e. saturating shorter or longer reduces effect. Here, we derive expressions this length. We also...
Signal amplification strategies are critical for overcoming the intrinsically poor sensitivity of nuclear magnetic resonance (NMR) reporters in noninvasive molecular detection. A mechanism widely used signal enhancement is chemical exchange saturation transfer (CEST) nuclei between a dilute sensing pool and an abundant detection pool. However, dependence CEST on relative size these spin pools confounds quantitative with larger typically making less efficient. Here we show that recently...
Nuclear magnetic resonance (NMR) spectroscopy and imaging (MRI) suffer from intrinsic low sensitivity because even strong external fields of ~10 T generate only a small detectable net-magnetization the sample at room temperature 1. Hence, most NMR MRI applications rely on detection molecules relative high concentration (e.g., water for biological tissue) or require excessive acquisition times. This limits our ability to exploit very useful molecular specificity signals many biochemical...
Abstract Caged xenon has great potential in overcoming sensitivity limitations for solution‐state NMR detection of dilute molecules. However, no application such a system as magnetic resonance imaging (MRI) contrast agent yet been performed with live cells. We demonstrate MRI localization cells labeled caged packed‐bed bioreactor working under perfusion hyperpolarized‐xenon‐saturated medium. Xenon hosts enable NMR/MRI experiments switchable and selectivity cell‐associated versus unbound...
We propose a method to dynamically monitor the progress of an enzymatic reaction using NMR hyperpolarized 129 Xe in host–guest system. It is based on displacement assay originally designed for fluorescence experiments that exploits competitive binding product one hand and reporter dye other supramolecular host. Recently, this has been successfully transferred NMR, xenon as reporter, cucurbit[6]uril host, chemical exchange saturation transfer with (Hyper‐CEST) detection technique. Its...
CEST has proven to be a valuable technique for the detection of hyperpolarized xenon-based functionalized contrast agents. Additional information can encoded in spectral dimension, allowing simultaneous multiple different biosensors. However, due low concentration dissolved xenon biological tissue, signal noise ratio (SNR) Hyper-CEST data is still critical issue. In this work, we present two techniques aiming increase SNR by exploiting typically high redundancy image series: PCA-based...
Xenon magnetic resonance imaging (MRI) provides excellent sensitivity through the combination of spin hyperpolarization and chemical exchange saturation transfer (CEST). To this end, molecular hosts such as cryptophane-A or cucurbit[
Localized detection of hyperpolarized, exchanging Xe spins enables quantitative insights at unprecedented sensitivity for characterizing chemical exchange kinetics in various contexts such as host–guest interactions and displacement assays.
Das NMR-Signal von hyperpolarisiertem 129Xe in Cryptophan-Käfigmolekülen erfährt unterschiedliche chemische Verschiebungen verschiedenen Lösungen. Eine neue Kodierungsmethode bezieht die reversible Xe-Bindung optimal ein, nutzt so effizient Hyperpolarisierung und bleibt dabei spektral hochselektiv. Gezeigt wird dies anhand MRI mit Xe-Wirtsstrukturen im nanomolaren Bereich, schneller Käfig-Molekül-Lokalisierung <1 s zeitaufgelösten Studien. Detailed facts of importance to specialist readers...
Nuclear magnetic resonance (NMR) spectroscopy and imaging (MRI) suffer from intrinsic low sensitivity because even strong external fields of ~10 T generate only a small detectable net-magnetization the sample at room temperature 1. Hence, most NMR MRI applications rely on detection molecules relative high concentration (e.g., water for biological tissue) or require excessive acquisition times. This limits our ability to exploit very useful molecular specificity signals many biochemical...
Hyperpolarisiertes 129Xe in Cryptophan-Käfigmolekülen erfährt unterschiedlichen Lösungsmitteln verschiedene chemische Verschiebungen, einem Fall mit einer Differenz von 131 Hz. In ihrer Zuschrift auf S. 8341 ff. stellen L. Schröder et al. eine Kodierungsmethode vor, welche die Hyperpolarisierung optimal für NMR-spektroskopische Aufnahme Schnappschüssen hoher spektraler Selektivität nutzt.
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