A5074474862- Advanced biosensing and bioanalysis techniques
- Epigenetics and DNA Methylation
- Advanced Biosensing Techniques and Applications
- Single-cell and spatial transcriptomics
- Biosensors and Analytical Detection
- RNA modifications and cancer
- RNA Interference and Gene Delivery
- ATP Synthase and ATPases Research
- Genetics and Neurodevelopmental Disorders
- Microfluidic and Bio-sensing Technologies
- Genomics and Chromatin Dynamics
- Cancer-related gene regulation
- Cancer Genomics and Diagnostics
- Click Chemistry and Applications
- Spectroscopy Techniques in Biomedical and Chemical Research
- DNA and Nucleic Acid Chemistry
University of Michigan
2020-2025
Analysis Group (United States)
2020-2024
Nankai University
2017
Collaborative Innovation Center of Chemical Science and Engineering Tianjin
2017
Nuclear delivery and accumulation are very important for many anticancer drugs that interact with DNA or its associated enzymes in the nucleus. However, it is difficult neutrally negatively charged such as 10-hydroxycamptothecine (HCPT). Here we report a simple strategy to construct supramolecular nanomedicines nuclear of dual synergistic drugs. Our utilizes coassembly HCPT-peptide amphiphile positively cisplatin. The resulting nanomaterials behave "Trojan Horse" transported soldiers...
ConspectusMethods for detecting and quantifying disease biomarkers in biofluids with high specificity sensitivity play a pivotal role enabling clinical diagnostics, including point-of-care tests. The most widely used molecular include proteins, nucleic acids, hormones, metabolites, other small molecules. While numerous methods have been developed analyzing biomarkers, techniques are challenging to implement use due insufficient analytical performance, cost, and/or practical shortcomings. For...
Abstract Early and personalized intervention in complex diseases requires robust molecular diagnostics, yet the simultaneous detection of diverse biomarkers—microRNAs (miRNAs), mutant DNAs, proteins—remains challenging due to low abundance preprocessing incompatibilities. We present Biomarker Single-molecule Chromato-kinetic multi-Omics Profiling Enumeration (Bio-SCOPE), a next-generation, triple-modality, multiplexed platform that integrates both chromatic kinetic fingerprinting for...
The most well-studied epigenetic marker in humans is the 5-methyl modification of cytosine DNA, which has great potential as a disease biomarker. Currently, quantification DNA methylation relies heavily on bisulfite conversion followed by PCR amplification and NGS or microarray analysis. subject to bias differential bisulfite-converted methylated versus unmethylated sequences. Here, we combine with single-molecule kinetic fingerprinting develop an amplification-free assay for at...
The most well-studied epigenetic marker in humans is the 5-methyl modification of cytosine DNA, which has great potential as a disease biomarker liquid biopsies cell-free DNA. Currently, quantification DNA methylation relies heavily on bisulfite conversion followed by PCR amplification and NGS or microarray analysis. subject to bias differential bisulfite-converted methylated
The most well-studied epigenetic marker in humans is the 5-methyl modification of cytosine DNA, which has great potential as a disease biomarker liquid biopsies cell-free DNA. Currently, quantification DNA methylation relies heavily on bisulfite conversion followed by PCR amplification and NGS or microarray analysis. subject to bias differential bisulfite-converted methylated versus unmethylated sequences. Here, we combine with single-molecule kinetic fingerprinting develop an...
DNA methylation is a fundamental element of epigenetic regulation that governed by the MBD protein superfamily, group "readers" share highly conserved methyl-CpG-binding domain (MBD) and mediate chromatin remodeler recruitment, transcription regulation, coordination histone modification. Previous work has characterized binding affinity sequence selectivity MBD-containing proteins toward palindromes 5-methylcytosine (5mC) containing 5mCpG dinucleotides, often referred to as single...