Nuclear delivery and accumulation are very important for many anticancer drugs that interact with DNA or its associated enzymes in the nucleus. However, it is difficult neutrally negatively charged such as 10-hydroxycamptothecine (HCPT). Here we report a simple strategy to construct supramolecular nanomedicines nuclear of dual synergistic drugs. Our utilizes coassembly HCPT-peptide amphiphile positively cisplatin. The resulting nanomaterials behave "Trojan Horse" transported soldiers...

Promising vaccine adjuvants of self‐assembling peptide hydrogels for protein ovalbumin (OVA) are introduced in this study. The formed by the enzyme phosphatase, and adjuvant potency both l ‐ d ‐peptide is evaluated. results indicate that, compared with clinically used alum adjuvant, can increase IgG production OVA about 1.3 3.8 times, respectively. Both gels enhance antigen uptake induce dendritic cell maturation, promote prolong accumulation lymph node, as well evoke germinal center...

Abstract The inherent immune and metabolic tumor microenvironment (TME) of most solid tumors adversely affect the antitumor efficacy various treatments, which is an urgent issue to be solved in clinical cancer therapy. In this study, a mitochondrial localized situ self‐assembly system constructed remodel TME by improving immunogenicity disrupting plasticity cells. peptide‐based drug delivery can pre‐assembled into nanomicelles vitro form functional nanofibers on mitochondria through...

An ideal radiosensitizer holding an enhanced tumor retention can play incredible role in enhancing radiotherapy. Herein, a strategy of acid-triggered aggregation small-sized gold nanoparticles (GNPs) system within is proposed and the resulting GNPs aggregates are applied as vitro vivo. The with achieves accumulation cancer cells tumors form resulted aggregates. As consequence, radiosensitization effect shows significant improvement radiotherapy, which shown studies DNA breakage comet assay,...

Chemo-photodynamic combination therapy has been received widespread attention in cancer treatment due to its excellent characteristics, such as reducing the adverse side effects of chemo-drugs and improving therapeutic for various cancers. In this study, RGD DOX was conjugated PEG by thiol-ene addition Schiff's base reaction, respectively, prepare targeted pH-sensitive antitumor prodrug nanoparticles (RGD-PEG-DOX NPs, RGD-NPs). Subsequently, photosensitizer chlorin e6 (Ce6) encapsulated into...

Abstract We demonstrate that the incorporation of one or two amino acids phenylalanine (F) 4‐fluoro ( f F) will greatly lower background fluorescence intensities conventional quenched probes with quenchers. This enhanced quenching effect was due to synergetic aggregation caused and presence a quencher. Such strategy not affect enzyme recognition properties probes. also demonstrated our self‐assembled nanoprobe showed better performance in cells for detection cell apoptosis than unassembled...

Abstract The increase of bacterial resistance demands rapid and accurate diagnosis infections. Biosurface‐induced supramolecular assembly for therapy has received little attention in detecting Herein we present a dual fluorescent‐nuclear probe based on self‐assembly vancomycin (Van) Gram‐positive bacteria imaging infection. A Van‐ rhodamine‐modified peptide derivative (Rho‐FF‐Van), as the agent, binds to terminal methicillin‐resistant staphylococcus aureus (MRSA) self‐assembles form...

Abnormal levels of alkaline phosphatase (ALP) activity are associated with various diseases, and many ALP probes have been developed to date. However, the development ALP-sensitive for living cells, especially detection bacterial ALP, remains challenging because complex dynamic context. In this study, we constructed first fluorescent probe (TPEPy-pY) sensing activity. TPEPy-pY is an AIEgen-peptide conjugate property aggregation-induced emission (AIE) could turn on its fluorescence by...

Abstract Cancer stem‐like cells (CSCs), capable of indefinite self‐renewal and differentiation, are considered to be the root cause tumor radiotherapy (RT) resistance. However, CSCs‐targeted therapy still remains a great challenge because they commonly located in deep making drugs hard approach, their hypoxic acidic niche can further aggravate radioresistance. Herein, based on finding that CSCs highly express carbonic anhydrase IX (CAIX) cell membrane, CAIX‐targeted induced situ...

Abstract Peptide‐based supramolecular therapeutics (PST) refer to those theranostic nanomedicines based on peptide or derivatives self‐assembly that are constructed in vitro vivo. Benefiting from the inherent advantages of peptides, such as good biocompatibility, high bioactivity, and flexible tunability, reported PST have shown excellent application prospects combating many major diseases including cancer, cardiovascular disease, infectious autoimmune metabolic so on. Through thoughtful...

Supramolecular hydrogels of self-assembling peptide–drug conjugates have been considered as effective self-delivery drug systems for cancer therapy in recent years. Here, a novel peptide-based supramolecular hydrogel was developed by simultaneously conjugating small-molecule chlorambucil (CRB) and peptide tyroservatide (YSV) to the peptide. The resulting with nanofiber structure showed enhanced stability against proteinase K degradation an improved cellular uptake performance comparison free...

As the prevalence of systemic fungal infections caused by Candida albicans gradually increases, it is necessary to explore potential and effective antifungals. Carvacrol reported be lethally toxic C. albicans, involving several mechanisms. However, form specific mechanism cell death this compound has not been delineated. In study, we found that carvacrol could significantly decrease survival rates, consistent with previous researches. Further examination proved treatment membrane...

Rationale: Tumor energy metabolism has been a well-appreciated target of cancer therapy; however, the change cells between oxidative phosphorylation and glycolysis poses challenge to above.In this study, we constructed an innovative mitochondrion-targeted supramolecular "nano-boat" based on peptide self-assembly for tumor combined chemo-radiotherapy by simultaneously inhibiting dual metabolism.Methods: A lipophilic self-assembled positively charged cyclen were integrated fabricate brand new...

Abstract Abundant glutathione (GSH) is a biological characteristic of lots tumor cells. A growing number studies are utilizing GSH depletion as an effective adjuvant therapy for tumor. However, due to the compensatory effect intracellular biosynthesis, hard be completely exhausted and strategy remains challenging. Herein, we report l -buthionine-sulfoximine (BSO)-based hypertoxic self-assembled peptide derivative (NSBSO) with dual functions biosynthesis inhibition selective ferroptosis...

Radiotherapy (RT) can produce a vaccine effect and remodel tumor microenvironment (TME) by inducing immunogenic cell death (ICD) inflammation in tumors. However, RT alone is insufficient to elicit systemic antitumor immune response owing limited antigen presentation, immunosuppressive microenvironment, chronic within the tumor. Here, novel strategy reported for generation of situ peptide-based nanovaccines via enzyme-induced self-assembly (EISA) tandem with ICD. As ICD progresses, peptide...

Here we reported on the first example of using disulfide bond as a cleavable linker to control molecular self-assembly and formation small (SM) hydrogels.

The resistance of tumor cells to anticancer drugs has become one the principal causes failure in clinical chemotherapy. To overcome this issue, developing feasible drug delivery systems for effective cancer therapy is urgently needed. In work, we construct an amphiphilic self-delivery system consisting Taxol and tyroservatide (YSV) resistance. carrier-free supramolecular hydrogel composed nanofibers formed by involved ester bond self-hydrolysis process, which high loading efficiency...

Abstract Self‐assembling peptide‐based nanoprobes have attracted increasing research interest in recent years because of their inherent advantages, such as good biosafety, flexible designability, ease synthesis, and degradability. Being precisely regulated by a delicate molecular design, the self‐assembly/disassembly behavior to external stimuli results signal turn‐on/off properties, which can therefore be applied for disease theranostics disease‐related detection, including cancer,...

The unique property of turning on their fluorescence after aggregation or assembly makes aggregation-induced emission luminogens (AIEgens) ideal luminescent molecules for the construction self-assembled peptide-based nanoprobes. However, characteristic highly twisted propeller-shaped molecular conformation AIEgens tends to prevent AIEgen-peptides. Here, we show that (i) distance between tetraphenylethene (TPE) and assembled peptides should not be too far (less than five glycines), otherwise...

As it is closely associated with tumor proliferation, metastasis, and the immunosuppressive microenvironment, dysfunctional Hippo pathway has become an extremely attractive target for treating multiple cancers. However, to date, corresponding chemotherapeutic nanomedicines have not been developed. Herein, a supramolecular self-delivery nanomedicine in situ transforming capacity was tailor-constructed Hippo-pathway restoration, its inhibitory effect against growth metastasis investigated...

Leukemia is a heterogeneous clonal disorder in which early hematopoietic cells fail to differentiate and do not undergo programmed cell death or apoptosis. Less than one-third of adult patients with leukemia are managed using current therapies due the emergence multidrug resistance (MDR), emphasizing need for newer more robust approaches. Recent reports have suggested that long non-coding RNAs (lncRNAs) contribute selective gene expression and, hence, could be manipulated effectively halt...

Here we report on self-assembled vancomycin derivatives for bacterial detection and inhibition simultaneously.