Xanthomonas oryzae pv. (Xoo) is widely prevalent and causes Bacterial Leaf Blight (BLB) in Basmati rice grown different areas of Pakistan. There a need to use environmentally safe approaches overcome the loss grain yield due this disease. The present study aimed develop inocula, based on native antagonistic bacteria for biocontrol BLB increase Super variety. Out 512 isolated from rhizosphere screened plant growth promoting determinants, isolate BRp3 was found be best as it solubilized 97 μg/...

The aim of this study was to isolate, characterize and use phosphate solubilizing bacteria enhance the bioavailability insoluble Ca-phosphate for wheat plants. For purpose, 15 phosphorus (PSB) were isolated from rhizospheric soils Peshawar southern Punjab region, Pakistan. These isolates identified using light microscopy 16S rRNA gene. Among bacteria, two strains (Pseudomonas sp. MS16 Enterobacter MS32) efficient P solubilizers based on their solubilization activity determined qualitatively...

The present study was conducted to evaluate the potential of rice rhizosphere associated antagonistic bacteria for growth promotion and disease suppression bacterial leaf blight (BLB). A total 811 rhizospheric were isolated screened against 3 prevalent strains BLB pathogen Xanthomonas oryzae pv. (Xoo) which five bacteria, i.e., Pseudomonas spp. E227, E233, Rh323, Serratia sp. Rh269 Bacillus Rh219 showed (zone inhibition 1-19 mm). Production siderophores found be common biocontrol determinant...

Basmati rice is famous for its flavor, aroma and long grain around the globe. Its demand increasing worldwide especially in Asia. However, production threatened by various problems faced field which results major crop losses. One of bacterial blight caused Xanthomonas oryzae pv. (Xoo). Xoo hijacks host machinery activating susceptibility genes (OsSWEET family genes), using endogenous transcription activator like effectors (TALEs). TALEs have effector binding elements (EBEs) promoter region...

Climate change augments the risk to food security by inducing drought stress and a drastic decline in global rice production. Plant growth-promoting bacteria (PGPB) have been known improve plant growth under stress. Here present study, we isolated, identified, well-characterized eight drought-tolerant from rhizosphere that are tolerant 20% PEG-8000. These strains exhibited multiple traits, i.e., 1-aminocyclopropane-1-carboxylic acid (ACC) deaminase activity, exopolysaccharide production,...

Phosphorous (P) deficiency is a major challenge faced by global agriculture. Phosphate-solubilizing bacteria (PSB) provide sustainable approach to supply available phosphates plants with improved crop productivity through synergistic interaction plant roots. The present study demonstrates an insight into this P-solubilizing mechanism of PSB isolated from rhizosphere soils wheat-growing agro-ecological zones Pakistan. Seven isolates were the efficient P solubilizers based on in vitro activity...

The present work was carried out to study the potential of plant rhizosphere associated bacteria for biocontrol potato black scurf disease caused by Rhizoctonia solani Khun AG-3. A total twenty-eight isolated from diseased and healthy plants grown in soil Naran Faisalabad, Pakistan were evaluated their antagonistic potential. Nine bacterial strains found be vitro, reduced fungal growth lysis sclerotia R. dual culture assay as well extracellular metabolite efficacy test. selected further...

An extensive use of chemical fertilizers has posed a serious impact on food and environmental quality sustainability. As the organic biofertilizers can satisfactorily fulfill crop's nutritional requirement, plants require less fertilizer application; hence, is low in residues environment polluted. The agriculture crop residues, being rich source nutrients, be used to feed soil crops after composting practicable approach sustainable waste management instead open-field burning residues. This...

Abstract This study was undertaken to isolate and characterize plant growth promoting bacteria (PGPB) occurring in four soils of Zanzibar, Tanzania as well evaluate their potential use biofertilizers for rice. A total 12 PGPB strains were isolated from rice studied characteristics, carbon/nitrogen source utilization patterns using QTS‐24 kits, phosphate solubilization, indole acetic acid (IAA) production, antibiotic resistance at different pH, temperature salt concentrations. All the...

AbstractPlant growth-promoting bacteria-mediated biocontrol of plant pathogens is renowned to enhance the growth plants using different direct or indirect mechanisms. The goal present investigation was evaluation Pseudomonas aeruginosa Z5 isolated from cotton grown in Pakistani soils for suppression Fusarium oxysporum associated with seedling disease. In dual culturing techniques, four bacterial strains inhibited fungal pathogens, i.e. F. oxysporum, moniliforme, solani and Rhizoctonia...

Plant Growth Promoting Rhizobacteria (PGPR) may be utilized to augment plant growth and suppress the pathogens. Objective: The present study was conducted isolate characterize antagonistic bacteria indigenous cotton sugarcane rhizosphere in Pakistan, evaluate their ability phytopathogenic Fusarium spp. Out of 63 isolates 37 different morphotypes were studied for activity against monoliformae, oxysporum solani. Among these 31 strains showed percentage suppression ranging from 40 66% spp.The...

Applying phosphate-solubilizing bacteria (PSB) as biofertilizers has enormous potential for sustainable agriculture. Despite this, there is still a lack of information regarding the expression key genes related to phosphate-solubilization (PS) and efficient formulation strategies. In this study, we investigated rock PS by Ochrobactrum sp. SSR (DSM 109610) relating it bacterial gene searching an formulation. The quantitative PCR (qPCR) primers were designed marker glucose dehydrogenase (gcd),...

Phosphorous (P) is a limiting macronutrient for crop growth. Its deficiency prevents plant development leading to an extensive use of phosphatic fertilizers globally. Bio-organic phosphate (BOP) fertilizer provides sustainable approach optimize nutrient availability, enhance yield, and mitigate the negative impacts chemical on environment. Therefore, present study integrates application heat-tolerant phosphate-solubilizing bacteria, rock phosphate, organic materials BOP. For this purpose,...

The climate change scenario has increased the severity and frequency of drought stress, which limits growth yield rice worldwide. There is a dire need to select drought-tolerant varieties sustain crop production under water scarcity. Therefore, present study effectively combined morpho-physiological biochemical approaches with technology infrared thermal imaging (IRTI) for reliable selection genotypes. Initially, we studied 28 genotypes including 26 advance lines three stress tolerance net...

Thirty rhizobacteria isolated from maize grown in Pakistani and Indonesian soils were evaluated for their morphological characteristics, nitrogen fixation, P-solubilization, indole acetic acid (IAA) siderophores production. Nitrogenase activity was detected nineteen isolates ranging 21.8-3624 n moles C2H4 produced/h/mg protein. Most of the produced IAA, ten capable siderophore production while four P-solubilizers. Ultrastructural studies Pseudomonas sp. F14 indicated characteristic...

SUMMARY The present investigation was designed to assess the range of growth-promoting activities various rhizosphere bacteria on wheat and oat growing in Lanzhou, China. Detection N-fixing by acetylene reduction assay-based most probable number (ARA-based MPN) method indicated presence significant numbers rhizobacteria, i.e. 5·8×10 6 bacteria/g dry weight root association with Chinese variety V4 . A total 24 rhizobacteria isolated from grown These bacterial isolates were studied for growth...