A5077634975- Reproductive Biology and Fertility
- Sperm and Testicular Function
- Renal and related cancers
- Animal Genetics and Reproduction
- Pluripotent Stem Cells Research
- Prenatal Screening and Diagnostics
- Ovarian function and disorders
- Reproductive Physiology in Livestock
- Reproductive Health and Technologies
- Epigenetics and DNA Methylation
- 3D Printing in Biomedical Research
- Assisted Reproductive Technology and Twin Pregnancy
- Genetic Syndromes and Imprinting
- Tissue Engineering and Regenerative Medicine
- Birth, Development, and Health
- Reproductive System and Pregnancy
- Spectroscopy Techniques in Biomedical and Chemical Research
- Bee Products Chemical Analysis
- Seed Germination and Physiology
- Genetic and phenotypic traits in livestock
- CRISPR and Genetic Engineering
- Genetic and Clinical Aspects of Sex Determination and Chromosomal Abnormalities
- Advanced Nanomaterials in Catalysis
- Pregnancy and preeclampsia studies
- Congenital Anomalies and Fetal Surgery
University of Sassari
2015-2025
Istituto Zooprofilattico Sperimentale della Sardegna
2009-2015
Yamaguchi University
2004
Kindai University
2004
Korea Research Institute of Bioscience and Biotechnology
2004
Agrifood Research Finland
2004
Ludwig-Maximilians-Universität München
2004
University College Dublin
2004
North Carolina State University
2004
State University of Norte Fluminense
2004
Preliminary experiments carried out on ovine oocytes were designed to establish correlations between activation protocols and subsequent rates of embryonic development. The best thereafter used in studies parthenogenesis cloning. first study established that chemical activators induce pronuclear development at a slightly higher rate than physical (ionomycin, 96% ethanol, 95% electro activation, 80%). Inhibition second polar body extrusion one single pronucleus observed the majority (~90%)...
The complete nuclear maturation and fertilization in vitro of oocytes from 30–40-day-old juvenile lambs, their ability to develop up the blastocyst stage when transferred into recipient ewes after vivo culture was studied. Cumulus–oocyte complexes were recovered ovaries only those with several cumulus cell layers selected compared adult sheep. rate meiotic progression significantly lower (P < 0.001) for (8%) than (58%) without gonadotrophins medium. However, a similar observed both (76%)...
Abstract The impact of vitrification procedures on in vitro matured (IVM) ovine oocytes mRNA content and ability to undergo successful fertilization, cleavage embronic development was assessed. Vitrified‐warmed (n = 113) control 140) IVM were fertilized cultured up blastocyst stage under standard conditions. Vitrified showed lower rate (47% vs. 75%, P < 0.001) (17% 57%, than controls. In addition, the timings first blastocysts production significantly delayed vitrified‐warmed group (...
Abstract We evaluated the effect of three different cryodevices on membrane integrity, tubulin polymerization, maturation promoting factor (MPF) activity and developmental competence in vitro matured (IVM) ovine oocytes. IVM oocytes were exposed during 3 min to 7.5% DMSO ethylene glycol (EG) TCM199 25 sec 0.5 M sucrose, 16.5% EG, loaded open pulled straws (OPS), cryoloops (CL) or cryotops (CT) immersed into liquid nitrogen. Untreated (CTR) vitrification solutions but not cryopreserved (EXP)...
Cerium oxide nanoparticles (CeO2 NPs) are able to store and release oxygen, conferring them scavenger activity against oxidative stress. However, their effects in reproductive systems not yet well understood. The aim of the study was investigate exposure refrigerated ram semen CeO2 NPs for 96 h on main structural kinematic parameters spermatozoa.The ejaculates 5 Sarda rams were collected, pooled diluted a soybean lecithin extender. Samples exposed increasing doses (0, 44 220 μg/mL) stored at...
It is known that a paracrine mechanism exists between mesenchymal stem cells and target cells. This process may involve microvesicles (MVs) as an integral component of cell-to-cell communication.In this context, study aims to understand the efficacy MVs in in-vitro endometrial stressed view potential healing in-vivo studies. For purpose, presence type secreted by amniotic (AMCs) were investigated response was studied using dose-response curve at different concentrations times. Moreover,...
Abstract The present study was conducted to investigate the relation between in vitro developmental competence and expression of a panel developmentally important genes germinal vesicle (GV) stage oocytes. One‐month‐old prepubertal adult sheep oocytes were used as models low high quality gametes, respectively. Cumulus‐oocyte complexes (COCs) derived from lambs ewes matured fertilized, their cleavage rate at 22, 26, 32 hr post fertilization blastocyst yield observed assess potential. In...
This study compares the developmental capacity and cryotolerance of embryos produced from oocytes stimulated prepubertal adult Sarda goats. Twelve 13 goats were each given 110 175 IU FSH, respectively, cumulus–oocyte complexes (COCs) collected by laparoscopic oocyte-pick-up (LOPU). After in vitro maturation, fertilisation culture (IVMFC), blastocysts vitrified, warmed blastocoel re-expansion gene expression evaluated. Prepubertal a higher COCs number than adults (mean ± s.e.m., 89.67 5.74...
The sub-cortical maternal complex (SCMC), located in the subcortex of mouse oocytes and preimplantation embryos, is composed at least four proteins encoded by effect genes: OOEP, NLRP5/MATER, TLE6 KHDC3/FILIA. SCMC assembles during oocyte growth was seen to be essential for murine zygote progression beyond first embryonic cell divisions; although roles chromatin reprogramming genome activation were hypothesized, full range functions development remains largely unknown.Here we report...