A5057234776- Neuroscience and Neuropharmacology Research
- Ion channel regulation and function
- Neuroinflammation and Neurodegeneration Mechanisms
- Cardiac electrophysiology and arrhythmias
- Muscle Physiology and Disorders
- Aldose Reductase and Taurine
- Signaling Pathways in Disease
- Cardiac Ischemia and Reperfusion
- Retinal Development and Disorders
- S100 Proteins and Annexins
- Cardiomyopathy and Myosin Studies
- Photoreceptor and optogenetics research
- Adipose Tissue and Metabolism
- Neurogenesis and neuroplasticity mechanisms
- RNA modifications and cancer
- RNA Research and Splicing
- Neuroscience and Neural Engineering
- Cardiovascular Effects of Exercise
- RNA Interference and Gene Delivery
- Cancer-related molecular mechanisms research
- Health and Medical Research Impacts
- Career Development and Diversity
- Exercise and Physiological Responses
- Connexins and lens biology
- Mitochondrial Function and Pathology
Salk Institute for Biological Studies
2020-2025
University of California, San Diego
2023-2025
University of Maryland, Baltimore
2006-2024
The University of Texas MD Anderson Cancer Center
2019
Unresectable glioblastoma (GBM) cells in the invading tumor edge can act as seeds for recurrence. The molecular and phenotypic properties of these remain elusive. Here, we report that core have two distinct types glioma stem-like (GSCs) resemble proneural (PN) mesenchymal (MES) subtypes, respectively. Upon exposure to ionizing radiation (IR), GSCs, initially enriched a CD133+ PN signature, transition CD109+ MES subtype C/EBP-β-dependent manner. Our gene expression analysis paired cohorts...
The transcription factor nuclear of activated T-cells 5 (NFAT5) is a key protector from hypertonic stress in the kidney, but its role skeletal muscle unexamined. Here, we evaluate effects glucose hypertonicity and hyperglycemia on endogenous NFAT5 activity, transverse tubular system morphology Ca 2+ signaling adult murine fibers. We found that exposure to elevated (25–50 mmol/L) increased expression translocation, NFAT-driven transcriptional activity. These were insensitive inhibition...
S100A1, a 21-kDa dimeric Ca 2+ -binding protein of the EF-hand type, is expressed in cardiomyocytes and an important regulator heart function. During ischemia, secrete S100A1 to extracellular space. Although effects have been documented cardiomyocytes, it unclear whether exerts modulatory on other tissues proximity with cardiac cells. Therefore, we sought investigate exogenous signals electrical properties superior cervical ganglion (SCG) neurons. Immunostaining Western blot assays indicated...
Summary The maturation and stabilization of appropriate synaptic connections is a vital step in the development neuronal circuits, however molecular signals underlying these processes are not fully understood. We show that astrocytes, through production glypican 5 (GPC5), required for refinement synapses developing mouse cortex. In absence astrocyte GPC5 thalamocortical visual cortex structural immaturity during critical period, including smaller presynaptic terminals, decreased postsynaptic...
The protein kinase PKD1 has recently been linked to slow fibre‐type gene expression in fast skeletal muscle through phosphorylation of class II histone deacetylase (HDAC) molecules, resulting nuclear efflux HDAC and consequent activation the transcription factor MEF2. However, possible upstream activators PKD, time course signalling pathway downstream effectors have not determined muscle. Using fluorescent fusion proteins HDAC5–green (GFP) PKD1–mPlum expressed fibres isolated from...
Abstract The transcription factor cAMP response element-binding protein (CREB) is involved in a myriad of cellular functions the central nervous system. For instance, role CREB via phosphorylation at amino-acid residue Serine (Ser)133 expressing plasticity-related genes and activity-dependent neuronal plasticity processes has been extensively demonstrated. However, much less known about Ser142 Ser143. Here, we employed viral vector containing dominant negative form CREB, with...
Significance A continuously evolving question regarding excitation–contraction coupling (ECC) in skeletal muscle has been around the mechanism that links activation of Ca V 1.1 voltage sensor with intracellular 2+ release. senses changes transmembranal through four distinct voltage-sensing domains (VSDs I–IV), yet whether one or all contribute to ECC is unknown. To investigate roles each VSD, this work introduces AP fluorometry track conformational sensors and clamp measure charge movement...
Abstract Fluorescent reporters for glutamate release and postsynaptic Ca 2+ signaling are essential tools quantifying synapse functional heterogeneity across neurons circuits. However, leveraging these probes neuroscience requires scalable experimental frameworks. Here, we devised a high-throughput approach to efficiently collect analyze hundreds of optical recordings glutaamate activity at presynaptic boutons in cultured rat hippocampal neurons. Boutons exhibited remarkable could be...
Astrocytes are glial cells of the central nervous system that modulate neuronal function. Here, we present glyoxal-fixed astrocyte nuclei transcriptomics (GFAT), a protocol for purification and transcriptomic analysis from cortex cerebellum adult aged fresh mouse brain. We describe steps tissue dissection, glyoxal fixation, homogenization, isolation, antibody staining, fluorescence-activated cell sorting, RT-qPCR or bulk RNA sequencing. GFAT does not require transgenic lines viral injection...
In excitation-contraction coupling (ECC), when the skeletal muscle action potential (AP) propagates into transverse tubules, it modifies conformational state of voltage-gated calcium channels (CaV1.1). CaV1.1 serves as voltage sensor for activation release from sarcoplasmic reticulum (SR); however, many questions about this function persist. α1 subunits contain four distinct homologous domains (I-IV). Each repeat includes six transmembranal helical segments; voltage-sensing domain (VSD) is...
The transcription factor NFAT5 is a key regulator in protection from hypertonic stress the renal medulla but its role skeletal muscle has not been examined. Since occurs during hyperglycemic crisis patients with diabetes, we sought to evaluate effects of sustained (1–2 days) and elevated glucose (25–50 mM) on endogenous activity Ca2+ signaling fibers culture. Exposure high increased nuclear translocation expression. NFAT-driven luciferase assays demonstrated an increase transcriptional...