A5082440302- RNA and protein synthesis mechanisms
- RNA Research and Splicing
- RNA modifications and cancer
- RNA regulation and disease
- Endoplasmic Reticulum Stress and Disease
- Autophagy in Disease and Therapy
- Neuroscience and Neuropharmacology Research
- Cellular Mechanics and Interactions
- Photoreceptor and optogenetics research
- Protein Structure and Dynamics
- Bioinformatics and Genomic Networks
- Erythrocyte Function and Pathophysiology
- 14-3-3 protein interactions
- Microbial Metabolic Engineering and Bioproduction
- Sexual Differentiation and Disorders
- Neurobiology and Insect Physiology Research
- Ubiquitin and proteasome pathways
- Cellular transport and secretion
- Genetics and Neurodevelopmental Disorders
- Galectins and Cancer Biology
- Intracranial Aneurysms: Treatment and Complications
Max Planck Institute for Brain Research
2019-2022
University of California San Diego Medical Center
2020
European Molecular Biology Laboratory
2016
Abstract Owing to their morphological complexity and dense network connections, neurons modify proteomes locally, using mRNAs ribosomes present in the neuropil (tissue enriched for dendrites axons). Although ribosome biogenesis largely takes place nucleus perinuclear region, neuronal ribosomal protein (RP) have been frequently detected remotely, axons. Here, imaging profiling, we directly RP translation neuropil. Combining brief metabolic labeling with mass spectrometry, found that a group...
At its most fundamental level, touch sensation requires the translation of mechanical energy into mechanosensitive ion channel opening, thereby generating electro-chemical signals. Our understanding this process, especially how cytoskeleton influences it, remains unknown. Here we demonstrate that mice lacking α-tubulin acetyltransferase Atat1 in sensory neurons display profound deficits their ability to detect stimuli. We show all cutaneous afferent subtypes, including nociceptors have...
To supply proteins to their vast volume, neurons localize mRNAs and ribosomes in dendrites axons. While local protein synthesis is required for synaptic plasticity, the abundance distribution of nascent near synapses remain elusive. Here, we quantified occurrence translation visualized range supplied by during basal plastic conditions. We detected dendritic at single-molecule resolution using DNA-PAINT metabolic labeling. Both positively correlated with synapse density. Ribosomes were ~85%...
Abstract During the co-translational assembly of protein complexes, a fully synthesized subunit engages with nascent chain newly interaction partner. Such events are thought to contribute productive assembly, but their exact physiological relevance remains underexplored. Here, we examine structural motifs contained in nucleoporins for potential facilitate assembly. We experimentally test candidate and identify several previously unknown interactions. demonstrate by selective ribosome...
We examined the feedback between major protein degradation pathway, ubiquitin-proteasome system (UPS), and synthesis in rat mouse neurons. When was inhibited, we observed a coordinate dramatic reduction nascent neuronal cell bodies dendrites. The mechanism for translation inhibition involved phosphorylation of eIF2α, surprisingly mediated by eIF2α kinase 1, or heme-regulated inhibitor (HRI). Under basal conditions, expression HRI is barely detectable. Following proteasome inhibition, levels...
Neurons are characterized by a complex morphology that enables the generation of subcellular compartments with unique biochemical and biophysical properties, such as dendrites, axons, synapses. To sustain these different carry wide array elaborate operations, neurons express diverse repertoire gene products. Extensive regulation at both messenger RNA (mRNA) protein levels allows for differentiation well numerous forms plasticity in response to variable stimuli. Among multiple mechanisms...
SUMMARY Owing to their morphological complexity and dense network connections, neurons modify proteomes locally, using mRNAs ribosomes present in the neuropil (tissue enriched for dendrites axons). Although ribosome biogenesis largely takes place nucleus perinuclear region, neuronal ribosomal protein (RP) have been frequently detected remotely, axons. Here, imaging profiling, we directly RP translation neuropil. Combining brief metabolic labeling with mass spectrometry, found that a group of...
Searchable abstracts of presentations at key conferences in endocrinology ISSN 1470-3947 (print) | 1479-6848 (online)
Abstract During the co-translational assembly of protein complexes, a fully synthesized subunit engages with nascent chain newly interaction partner. Such events are thought to contribute productive assembly, but their exact physiological relevance remains underexplored. Here, we examined structural motifs contained in nucleoporins for potential facilitate assembly. We experimentally tested candidate and identified several previously unknown interactions. demonstrate by selective ribosome...
Abstract All cells, including neurons, have regulatory feedback mechanisms that couple protein synthesis and degradation to maintain optimize concentrations in the face of intra- extracellular perturbations. We examined between major pathway, ubiquitin-proteasome system (UPS), neurons. When by UPS was inhibited we observed a coordinate dramatic reduction nascent both neuronal cell bodies dendrites. The mechanism for translation inhibition involved phosphorylation eIF2a, surprisingly mediated...