The co-carcinogenic compound phorbol 12-myristate 13-acetate but not its inactive analogue 4 alpha-phorbol 12,13-didecanoate causes the phosphorylation of several rabbit neutrophil polypeptides whose molecular weights and isoelectric points (pI) are as follows: Mr = 40,000, pI 6.4; 50,000, 4.9; 55,000, 6.3; 64,000, 6.0; 70,000, 5.6; 90,000, 6.0. Most these phosphorylated proteins located exclusively in cytosol; 64,000 weight protein is found both cytosol cytoskeleton, 40,000 nuclear pellet....

Addition of pertussis toxin to rabbit neutrophils inhibits the fMet-Leu-Phe- induced increases in Na+ influx and intracellular pH. In addition, pretreatment cells with decrease levels phosphatidylinositol 4,5-bisphosphate 4-phosphate enhanced production phosphatidic acid produced by chemotactic factor fMet-Leu-Phe. Furthermore, fMet-Leu-Phe-induced changes phosphorylation a 46-kDa protein several other proteins are also inhibited toxin. On hand, phorbol 12-myristate 13-acetate (PMA)-induced...

// Meng Han 1 , Peixia Song Chang Huang Arash Rezaei 2 Shabnam Farrar 3 Michael A. Brown 4 and Xi Ma 1,5 State Key Laboratory of Animal Nutrition, China Agricultural University, Beijing, School Medicine, University Central Florida, Orlando, FL, USA College Dental Midwestern Downers Grove IL, Department Science, Oklahoma Stillwater, OK, 5 Internal Biochemistry, Center for Autophagy Research, Texas Southwestern Medical Center, Dallas, TX, Correspondence to: Ma, email: Keywords : grape seed...

The protein tyrosine phosphatase (PTPase) inhibitor pervanadate (vanadyl hydroperoxide) stimulated phosphorylation 29-fold more than did thrombin in intact and saponin-permeabilized platelets. Increased preceded, or was coincident with, a fall PtdIns(4,5)P2 levels, production of PtdIns(3,4)P2 phosphatidic acid, mobilization intracellular Ca2+, stimulation kinase C-dependent phosphorylation, secretion dense alpha-granules, increased actin polymerization, shape change aggregation which...

The addition of platelet-activating factor (PAF) to human neutrophils increases the levels tyrosine phosphorylation in several proteins. These proteins have molecular weights 41 (pp41), 54 (pp54), 66 (pp66), 104 (pp104), and 116 (pp116) kDa. effect PAF was dose-dependent could be seen at concentrations as low 1 nM. nonmetabolizable bioactive analog, C-PAF, caused an increase level same a time- manner. On contrary, lyso-PAF, enantio-PAF, L-beta,gamma-dihexadecyl-alpha-lecithin failed...

The addition of granulocyte-macrophage colony-stimulating factor (GM-CSF) to human neutrophils causes a rapid increase in the basal and fMet-Leu-Phe-stimulated Na+ influx an intracellular pH. can be seen as early 5 min after GM-CSF. Changes produced by GM-CSF are totally inhibited amiloride significantly reduced pertussis toxin-treated cells. stimulation Na+/H+ exchange mechanism inhibits further this system with either fMet-Leu-Phe or phorbol 12-myristate 13-acetate. In addition, membrane...

F Met-Leu-[3H]Phe and f Nle-Leu-[3H]Phe binding to rabbit peritoneal neutrophils purified membranes were measured at 4 degrees C silicone oil centrifugation assays, the results analyzed by LIGAND computer program, which permits analysis of ligand multiple classes sites. peptide intact neutrophil indicated that both detected two population The apparent Kd values for 1.6 +/- 1.0 X 10(-9) M 2.2 0.9 10(-8) M, respectively, 3.1 0.2 1.2 0.6 10(-7) Nle-Leu-[3H]Phe. Furthermore, higher affinity...

Porcine reproductive and respiratory syndrome (PRRS) is one of the most economically devastating diseases affecting pork industry globally. PRRS caused by virus (PRRSV). Currently there are no effective treatments against this swine disease.Through artificial intelligence molecular screening, we obtained a set small molecule compounds predicted to target scavenger receptor cysteine-rich domain 5 (SRCR5) CD163, which cell surface specific for PRRSV infection. These were screened using...

Activation of T lymphocytes leads to the production cell growth factor IL-2 that regulates proliferation. This activation is associated with several potential intracellular signalling events including increased activity phospholipase C (PLC) and resultant increases in inositol phosphates diacylglycerols. In addition, phosphorylation specific proteins on serine, threonine, tyrosine residues increases. The role each these unclear. Using Western blotting antiphosphotyrosine antibodies, we...

ABSTRACT Human cell reprogramming remains extremely inefficient and the underlying mechanisms by different factors are elusive. We found that NANOG LIN28 (NL) synergize to improve OCT4, SOX2, KLF4 MYC (OSKM)-mediated ∼76-fold shorten latency at least 1 week. This synergy is inhibited GLIS1 but reinforced an inhibitor of histone methyltransferase DOT1L (iDOT1L) a ∼127-fold increase in TRA-1-60-positive (+) iPSC colonies. Mechanistically, NL serve as main drivers epithelialization, expression...

Hyperimmune immunoglobulin (hCoV-2IG) generated from SARS-CoV-2 convalescent plasma (CP) are under evaluation in clinical trials. Here we explored the antibody epitope repertoire, and virus neutralizing capacity of six hCoV-2IG batches as well nine CP against emerging variants concern (VOCs). Epitope-mapping by gene-fragment phage display library spanning spike demonstrated broad recognition multiple antigenic sites entire that was higher for than CP, with predominant binding to fusion...

Protein tyrosine phosphorylation in rabbit peritoneal neutrophils was examined by immunoblotting with antibodies specific for phosphotyrosine. Stimulation of the chemotactic factor fMet-Leu-Phe (10 nM) caused rapid increases several proteins apparent molecular masses (Group A) 54-58 kDa and 100-125 B) 36-41 kDa. Group A observed nM, maximum at 20 s) A23187 (1 microM, 1 min). B (ED50 0.15 min), leukotriene B4 phorbol 12-myristate 13-acetate (PMA) 25 ng/ml, 10 min) partially ionophore...

Granulocyte-macrophage colony-stimulating factor (GM-CSF), formylmethionylleucylphenylalanine, tumor necrosis alpha, platelet-activating factor, phorbol ester (phorbol 12-myristate 13-acetate), and calcium ionophore A23187 are able to increase the level of tyrosine phosphorylation different protein substrates, as demonstrated by Western blotting with anti-phosphotyrosine antibody (anti-PY). A 41 kDa (p41) consistently showed more intense reactivity anti-PY than controls. Blots treated...

Human polymorphonuclear neutrophils exhibit a low level of the microtubule-associated protein kinase (MAPK) activity. This enzymic activity is enhanced up to 3-fold upon cell stimulation with human haematopoietic hormone granulocyte-macrophage colony-stimulating factor (GM-CSF). demonstrated both in whole-cell lysates and DEAE-anion-exchange semi-purified fractions prepared from GM-CSF-stimulated neutrophils, by assaying against either myelin basic or phosphoacceptor peptide that bears...

Rabbit peritoneal neutrophils were reacted for 5-10 s with the chemotactic factors, fMet-Leu-Phe or (5S),(12R)-dihydroxy-6,8,11,14-(cis,trans,trans,cis)-eicosatetraenoic acid and then lysed a solution of Triton X-100 [gamma-32P]ATP. They showed an enhanced incorporation 32P in Mr = 60,000- 67,000-dalton polypeptides compared to control cells treated similarly. Another factor, C5a, produced similar but much lesser effect. The enhancement was not affected by addition purified catalytic subunit...

The objective of this study was to evaluate the effects particle size and drying methods corn on growth performance weaned piglets. Crossbreed piglets (n = 192; Duroc × Landrace Large White) were assigned one four treatments (2 2 factorial arrangement). All fed corn–soybean meal diets (1) hot air-dried coarsely ground corn, (2) finely (3) sun-dried (4) corn. results showed that (FGC) improved Additionally, apparent total tract digestibility (ATTD) gross energy (GE) ether extract (EE)...

Mucosal SARS-CoV-2 immune profiles are divergent in asymptomatic versus symptomatic SARS-CoV-2–infected individuals.

The generation of induced pluripotent stem cells (iPSCs) has underdefined mechanisms. In addition, leukemia inhibitory factor (LIF) activated Janus kinase/signal transducer and activator transcription 3 (JAK/STAT3) pathway is the master regulator for naïve-state pluripotency achievement maintenance. However, regulatory process to attain naïve iPSCs not well understood. We performed transcriptome analysis dissect genomic expression during mouse iPSC induction, with or without blocking...