A5101951622- Advanced Biosensing Techniques and Applications
- Ubiquitin and proteasome pathways
- RNA and protein synthesis mechanisms
- Advanced Proteomics Techniques and Applications
- Monoclonal and Polyclonal Antibodies Research
- Protein Structure and Dynamics
- Immune Response and Inflammation
- Advanced biosensing and bioanalysis techniques
- Bacteriophages and microbial interactions
- Advanced Fluorescence Microscopy Techniques
- Influenza Virus Research Studies
- Click Chemistry and Applications
- Pancreatic function and diabetes
- Enzyme Structure and Function
- Protein Degradation and Inhibitors
- Chemical Synthesis and Analysis
- RNA Research and Splicing
- Chemokine receptors and signaling
- Antimicrobial Peptides and Activities
- Amino Acid Enzymes and Metabolism
- Immunotherapy and Immune Responses
- Cardiac Imaging and Diagnostics
- Fungal and yeast genetics research
- Cellular transport and secretion
- Biochemical and Structural Characterization
Korea Institute of Science and Technology
2018-2024
Yonsei University
2023-2024
Wonju Severance Christian Hospital
2023
University of Toronto
2016-2018
Korea Advanced Institute of Science and Technology
2007-2014
The intrinsically disordered regions of eukaryotic proteomes are enriched in short linear motifs (SLiMs), which crucial relevance for cellular signaling and protein regulation; many mediate interactions by providing binding sites peptide-binding domains. vast majority SLiMs remain to be discovered highlighting the need experimental methods their large-scale identification. We present a novel proteomic peptide phage display (ProP-PD) library that displays peptides representing human proteome,...
Glucokinase (GK) is a monomeric allosteric enzyme and plays pivotal role in blood glucose homeostasis. GK regulated by regulatory protein (GKRP), indirectly effectors of GKRP. Despite the critical roles GKRP, molecular basis for regulation mechanism GKRP remains unclear. We determined crystal structure Xenopus complex presence fructose-6-phosphate at 2.9 Å. binds to super-open conformation mainly through hydrophobic interaction, inhibiting activity locking small domain GK. demonstrate...
Advanced ScienceVolume 12, Issue 1 2570003 Inside Back CoverOpen Access Massively Parallel Screening of Toll/Interleukin-1 Receptor (TIR)-Derived Peptides Reveals Multiple Toll-Like Receptors (TLRs)-Targeting Immunomodulatory (Adv. Sci. 1/2025) Yun Lim, LimSearch for more papers by this authorTae Kyeom Kang, Tae KangSearch authorMeong Il Kim, Meong KimSearch authorDohyeon Dohyeon authorJi Yul Ji authorSang Hoon Jung, Sang JungSearch authorKeunwan Park, Keunwan ParkSearch authorWook-Bin Lee,...
Analysis of protein dynamics using single-molecule fluorescence resonance energy transfer (smFRET) is widely used to understand the structure and function proteins. Nonetheless, site-specific labeling proteins with a pair donor acceptor dyes still remains challenge. Here we present general facile method for dual by incorporating two different, readily available, unnatural amino acids (p-acetylphenylalanine alkynyllysine) smFRET. We newly evolved alkynyllysine-specific aminoacyl-tRNA...
Combining the power of combinatorial screening and computational design for optimal protein engineering.
Cholangiocarcinoma (CCA) is a silent tumor with high mortality rate due to the difficulty of early diagnosis and prediction recurrence even after timely surgery. Serologic cancer biomarkers have been used in clinical practice, but their low specificity sensitivity problematic. In this study, we aimed identify CCA-specific glycan epitopes that can be for elucidate mechanisms by which glycosylation altered progression.The serum patients various cancers was fractioned into membrane-bound...
Aim The aim of this study was to assess antibacterial activity a novel Bacillus velezensis strain NST6, and further identify its active compound against pathogenic Staphylococcus strains for clinical therapeutic applications. Methods Results In study, B. NST6 harbouring strong antimicrobial human bacteria isolated from soil sample. solvent extract the exhibited Gram-positive Gram-negative in disc diffusion assay measurement minimal inhibitory concentration bactericidal concentration, which...
Small GTPases are key signaling nodes that regulate the cellular processes and subcellular events, their abnormal activities dysregulations closely linked with diverse cancers. Here, we report development of conformation-selective protein binders for a KRAS mutant. The conformation-specific were selected from repebody scaffold composed LRR (Leucine-rich repeat) modules through phage display modular engineering against constitute active conformation KRAS. Epitope was mapped to be located...
The aim of this study was to evaluate whether the N-terminus human serum albumin (HSA) has a role in cobalt binding detected using cobalt-binding (ACB) assay. We compared results obtained an enzyme-linked immunosorbent assay (ELISA) for N-terminal-modified HSA with those conventional ACB two groups: acute coronary syndrome (n = 43) and non-ischemic chest pain 39). cardiac troponin-I levels were higher group. No significant correlation between ELISA observed either patient groups. In...
This study investigates a real-time handheld bioaerosol monitoring system for the detection of biological particles using UV-LED and light-induced fluorescence technology. Biological produce both scattering signals simultaneously, which can help distinguish them from general particles. The detected scattering, fluorescence, simultaneous are then converted into photon categorized based on predetermined criteria. A reliable particle generator was required to validate performance system....
Studies on glutamine (Gln) metabolism have highlighted the vital role of Gln in cellular functions and its potential as a biomarker for disease detection. Despite increasing interest metabolism, in-depth evaluations are challenging owing to limitations conventional Gln-measuring methods. Thus, we developed ligand-induced dimerization-based sensor Gln, termed Q-SHINE, by splitting glutamine-binding protein into two separate domains. Q-SHINE enables highly accurate convenient measurement...
Cyanobacteriochromes (CBCRs) are linear tetrapyrrole bilin-binding photoreceptors of cyanobacteria that exhibit high spectral diversity, gaining attention in optogenetics and bioimaging applications. Several engineering studies on CBCRs were attempted, especially for designing near-infrared (NIR) fluorescent proteins with longer fluorescence wavelengths. However, despite continuous efforts, a key component regulating emission property is still poorly understood. As model system, we focused...
천연물 기반 항바이러스제는 다양한 바이러스 질환, 특히 COVID-19와 같은 신종 바이러스에 대응할 수 있는 강력한 치료제 후보로 주목받고 있다. 본 리뷰에서는 SARS-CoV-2의 효소 억제 효능을 갖는 천연물들에 대해 정리해 보았다. flavonoid나 polyphenol등의 화합물들이 SARS-CoV-2 Mpro 및 RdRp에 좋은 보이는 것을 알 있었다. 하지만 이들 천연물로부터 항바이러스제를 개발함에 있어서 아직 넘어야 할 한계들이 기존 약물 대비 작은 효능, 낮은 생이용성, 원료 수급의 어려움 등이다. 그럼에도 불구하고 많은 천연물들은 효능 뿐만 아니라 항염증 등도 보여서 기존의 항바이러스와의 병용제로 개발 될 가능성이 있다고 또한 유도체화, 나노 제형화, 합성법 등과 전략을 통해 천연물의 산업적 가치를 높일 있다면 항바이러스제로 있을 것으로 기대된다.
Abstract Toll-like receptors (TLRs) are critical regulators of the immune system, and altered TLR responses lead to a variety inflammatory diseases. Interference intracellular signaling, which is mediated by multiple Toll/interleukin-1 receptor (TIR) domains on all TLRs adapters, an effective therapeutic strategy against dysregulation. Peptides that inhibit TIR-TIR interactions fragmenting interface residues have potential as decoys. However, systematic method for discovering TIR-targeting...
Abstract Toll‐like receptors (TLRs) are critical regulators of the immune system, and altered TLR responses lead to a variety inflammatory diseases. Interference intracellular signaling, which is mediated by multiple Toll/interleukin‐1 receptor (TIR) domains on all TLRs adapters, an effective therapeutic strategy against dysregulation. Peptides that inhibit TIR‐TIR interactions fragmenting interface residues have potential as decoys. However, systematic method for discovering TIR‐targeting...