Euphorbia cuneata Vahl. (Euphorbiaceae) is a plant used in folk medicine for the treatment of pain and inflammation, although biological basis these effects has not been thoroughly investigated. The goal this study was to investigate pharmacological properties characterization phenolic flavonoid compounds present aerial parts E. cuneata. were tested antioxidant activity (DPPH), antibacterial activity, cell viability cytotoxic effects, anti-inflammatory activity. Phenolic contents (HPLC),...

Solenostemma argel is a desert medicinal plant indigenous to African countries. This research aims study the pharmacological properties of plant. Aerial parts (leaves and flowers) (Delile) Hayane were tested for antibacterial activity, antioxidant anticancer, anti-inflammatory activity. Phenolic flavonoid contents characterized. There was an increase in activity extract from 12.16% 94.37% by increasing concentration from10 µg/mL 1280 µg/mL. The most sensitive organism S. epidermidis with...

A Malus domestica MdMYB10 transcription factor gene was previously used as visible marker for successful plant transformation. We combined the with a GFP to test its viability non-destructive, visual, double reporter system functional promoter studies in transgenic strawberry plants. The fused provide evidence activity red colored cells of transformed tissue and exclude artefacts resulting from stress response or due other environmental cues. To this first approach, we evaluated...

An  improved  plant  regeneration and Agrobacterium-mediated genetic transformation system was established for squash (Cucurbita pepo L.) cucumber (Cucumis sativus L.). The effect of 2,4-D kinetin on callus formation, the liquid solid MS medium embryogenic proliferation, expression Gus gene beside its influence by acetosyringone, regeneration transformed non-transformed plant cells were investigated. Cotyledon explants 1 mg/L found to be best induction formation giving highest percentage;...

Sweet sorghum is an emerging biofuel candidate crop with multiple benefits as a source of biomass energy. Increase and sugar productivity quality central goal in its improvement. Target region amplified polymorphism (TRAP) polymerase chain reaction (PCR) based functional marker system that can detect genetic diversity the target genes. Thirty sweet genotypes were used to study potential 24 pairs TRAP assessing regard three lignin sucrose biosynthesis genes.A total 1638 bands produced out...

Cucumber (Cucumis sativus L. cv. Faris) explants were transformed by LBA4404 strain of Agrobacterium tumefaciens harboring the binary vector pPZPnptCat-WMV. The T-DNA region contains Neomycin Phosphotransferase II (NPT II) as a selectable marker gene and sequences Watermelon Mosaic Virus-II (WMV-II) fused with Green Fluorcent Protein (GFP) under control 35S promoter. Agrobacterium-mediated transformation was optimized using GFP reporter gene. parameters concentration, preculture period,...

Twenty-six potato genotypes including seventeen cultivars were used to investigate the genetic diversity of plants (Solanum tuberosum L.) grown in Egyptian sandy soil using both morphological characteristics and molecular markers. The analysis variance (ANOVA) yield traits its components showed highly significant differences among genotypes, growing seasons interaction between for all studied traits. NAP geno type gave highest values percentage tuber size more than 70 mm diameter trait. NIZ...

Background: The p7-transactivated protein1 of Hepatitis C virus is a small integral membrane protein 127 amino acids, which crucial for assembly and release infectious virions. Ab initio or comparative modelling, an essential tool to solve the problem structure prediction comprehend physicochemical fundamental how proteins fold in nature. Results: Only one domain (1-127) has been predicted using systematic silico approach, ThreaDom. I-TASSER was ranked as best server full-length 3-D...

Four Egyptian faba bean (Vicia L.) varieties ("Giza843", "Giza716", "Misr1" and "Sakha1") have been evaluated under heat drought stresses using seventeen morphological traits total protein content. Analysis of variance the trait revealed highly significant differences among treatments genotypes for all studied interaction between treatment was also significant. Both "Giza716" "Giza843" gave highest means most traits. The control surpass other in lowest were obtained from with while effect on...

Commercial cucumber cultivars were explored for embryogenesis and plant regeneration induced in somatic tissues on growth regulators.An efficient vitro protocol Cucumis sativus L via has been developed.Embryogenic callus cultures established from mature seeds, cotyledons shoot tips Murashige Skoog (MS) medium containing 87.64 µM sucrose, 0.8% agar, 2,4-dichlorophenoxyacetic acid 2,4-D kinetin.Maximum induction (94%, 92%) was observed seeds MS supplemented with 1mg/L 2,4-D,...

Abstract Solenostemma argel is a desert medicinal plant indigenous to African countries. The goal of this work was investigate the pharmacological properties phenolic and flavonoid compounds present in . Aerial parts (leaves flowers) were tested for antibacterial activity, antioxidant cell viability cytotoxic effects, anti-inflammatory activity. Phenolic contents (HPLC), volatile constituents (GC-MS) also characterized. activity extract increased from (34.47%) at concentration 10 µg/mL...

Most plant genes contain intervening sequences known as introns that are found in nearly all and they transcribed into pre-mRNA, the later will be removed by splicing machinery.Proteins encoded exons which separated intron segments while noncoding conserved mature transcript.In this study, level of spliced non-spliced was measured transgenic Nicotiana tabacum lines expressing gusint-gfpint fusion gene controlled CaMV 35S promoter.Transcription analysis transgenes performed (RT)-PCR.All...