A5006198339- Cancer Immunotherapy and Biomarkers
- CAR-T cell therapy research
- Immunotherapy and Immune Responses
- Ferroptosis and cancer prognosis
- Cancer Cells and Metastasis
- Pancreatic and Hepatic Oncology Research
- Immune cells in cancer
- Immune Cell Function and Interaction
- Single-cell and spatial transcriptomics
- Cancer Research and Treatments
- Cancer Genomics and Diagnostics
- Brain Metastases and Treatment
- Systemic Lupus Erythematosus Research
- Glioma Diagnosis and Treatment
- Meningioma and schwannoma management
- T-cell and B-cell Immunology
Yale University
2018-2023
Abstract Purpose: To determine the tumor tissue/cell distribution, functional associations, and clinical significance of PD-1, LAG-3, TIM-3 protein expression in human non–small cell lung cancer (NSCLC). Experimental Design: Using multiplexed quantitative immunofluorescence, we performed localized measurements CD3, >800 clinically annotated NSCLCs from three independent cohorts represented tissue microarrays. Associations between marker's major genomic alterations were studied The...
Abstract Specific mechanisms by which tumor-infiltrating lymphocytes (TIL) become dysfunctional remain poorly understood. Here, we employed a two-pronged approach using single-cell mass cytometry and tissue imaging technologies to dissect TILs from 25 patients with resectable 35 advanced non–small cell lung cancer (NSCLC). We identified burned-out CD8+ TIL subset (Ebo) that specifically accumulated within the tumor microenvironment (TME) but not in adjacent nontumoral tissues. Ebo showed...
Systemic lupus erythematosus (SLE) and discoid (DLE) of the skin are autoimmune diseases characterized by inappropriate immune responses against self-proteins; key elements that determine disease pathogenesis progression largely unknown. Here, we show mice lacking inhibitory receptor VISTA or programmed death-1 homolog (PD-1H KO) on a BALB/c background spontaneously develop cutaneous systemic resembling human lupus. Cutaneous lesions PD-1H KO have clustering plasmacytoid dendritic cells...
Abstract Background Malignant meningiomas are fatal and lack effective therapy. As M2 macrophages the most prevalent immune cell type in human meningiomas, we hypothesized that normalizing this immunosuppressive population would be an treatment strategy. Methods We used CIBERSORTx to examine proportions of 22 subsets meningiomas. targeted colony-stimulating factor 1 (CSF1) or CSF1 receptor (CSF1R) axis, important regulator macrophage phenotype, using monoclonal antibodies (mAbs) a novel...
Acute myeloid leukemia (AML) presents a pressing medical need in that it is largely resistant to standard chemotherapy as well modern therapeutics such targeted therapy and immunotherapy, including anti-PD therapy. We demonstrate Programmed Death-1 Homolog (PD-1H), an immune co-inhibitory molecule highly expressed blasts from the bone marrow of AML patients, while normal cell subsets T cells have expression PD-1H. In studies employing syngeneic humanized mouse models, overexpression PD-1H...
<h3>Background</h3> Cancer-associated fibroblasts (CAFs) are a major component of the non-small cell lung cancer (NSCLC) tumor microenvironment (TME).<sup>1–4</sup> Recent studies indicate that CAFs play role in generating CD8+T (CTL)-exclusive TME.<sup>5–9</sup> Given immune-checkpoint inhibitors (ICIs) rely on CTLs, an abundance TME may result reduced ICI efficacy. Although considered potential targets for therapy, attempts to deplete have largely failed.<sup>10</sup> This is due fact...
<p>Supplementary Figures and Tables</p>
<div>AbstractPurpose:<p>To determine the tumor tissue/cell distribution, functional associations, and clinical significance of PD-1, LAG-3, TIM-3 protein expression in human non–small cell lung cancer (NSCLC).</p>Experimental Design:<p>Using multiplexed quantitative immunofluorescence, we performed localized measurements CD3, >800 clinically annotated NSCLCs from three independent cohorts represented tissue microarrays. Associations between marker's major genomic...
<div>AbstractPurpose:<p>To determine the tumor tissue/cell distribution, functional associations, and clinical significance of PD-1, LAG-3, TIM-3 protein expression in human non–small cell lung cancer (NSCLC).</p>Experimental Design:<p>Using multiplexed quantitative immunofluorescence, we performed localized measurements CD3, >800 clinically annotated NSCLCs from three independent cohorts represented tissue microarrays. Associations between marker's major genomic...
<p>Supplementary Figures and Tables</p>
<div>Abstract<p>Specific mechanisms by which tumor-infiltrating lymphocytes (TIL) become dysfunctional remain poorly understood. Here, we employed a two-pronged approach using single-cell mass cytometry and tissue imaging technologies to dissect TILs from 25 patients with resectable 35 advanced non–small cell lung cancer (NSCLC). We identified burned-out CD8<sup>+</sup> TIL subset (Ebo) that specifically accumulated within the tumor microenvironment (TME) but not in...
<div>Abstract<p>Specific mechanisms by which tumor-infiltrating lymphocytes (TIL) become dysfunctional remain poorly understood. Here, we employed a two-pronged approach using single-cell mass cytometry and tissue imaging technologies to dissect TILs from 25 patients with resectable 35 advanced non–small cell lung cancer (NSCLC). We identified burned-out CD8<sup>+</sup> TIL subset (Ebo) that specifically accumulated within the tumor microenvironment (TME) but not in...
<p>Supplementary Figure Legens</p>
<p>Figures S1-14 and Legends</p>
<p>Figures S1-14 and Legends</p>
<p>Supplementary Figure Legens</p>