A5072990295- Ion channel regulation and function
- Ion Transport and Channel Regulation
- Plant Pathogens and Fungal Diseases
- Erythrocyte Function and Pathophysiology
- Dermatology and Skin Diseases
- Biochemical Analysis and Sensing Techniques
- Plant Pathogens and Resistance
- Hydrology and Watershed Management Studies
- Pancreatic function and diabetes
- Ion Channels and Receptors
- Groundwater flow and contamination studies
- Soil and Water Nutrient Dynamics
- Neuropeptides and Animal Physiology
- Advancements in Transdermal Drug Delivery
- Salivary Gland Disorders and Functions
- Neurobiology and Insect Physiology Research
- Receptor Mechanisms and Signaling
- Wheat and Barley Genetics and Pathology
- Biomedical Text Mining and Ontologies
- Photoreceptor and optogenetics research
- Electronic Health Records Systems
- Endodontics and Root Canal Treatments
- Dental Radiography and Imaging
- Olfactory and Sensory Function Studies
- Mycotoxins in Agriculture and Food
Unilever (United Kingdom)
2003-2025
University of Rochester Medical Center
1999-2020
National Park Service
2019
Sinclair Pharma
2011
Temple University
1964-2006
Sinclair Dermatology
2006
Pennsylvania State University
2001-2005
Penn State Milton S. Hershey Medical Center
2001
Institute of Biomedical Science
2000
University of Rochester
1998-2000
Aggregation of the high affinity receptor for immunoglobulin E (Fc epsilon RI) on mast cells results in rapid tyrosine phosphorylation and activation Syk, a cytoplasmic protein kinase. To examine role Syk Fc RI signaling pathway, we identified variant RBL-2H3 that has no detectable by immunoblotting vitro kinase reactions. In these Syk-deficient TB1A2 cells, aggregation induced histamine release increase total cellular phosphorylation. However, stimulation with calcium ionophore did induce...
In most cells, the ubiquitously expressed Na + /H exchanger isoform 1 (NHE1) is thought to be a primary regulator of pH homeostasis, cell volume regulation, and proliferative response growth factor stimulation. To study function NHE1 during embryogenesis when these cellular processes are very active, we targeted Nhe1 gene by replacing sequence encoding transmembrane domains 6 7 with neomycin resistance gene. NHE activity assays on isolated acinar cells indicated that allele functionally...
The salivary fluid secretory mechanism is thought to require Na<sup>+</sup>/K<sup>+</sup>/2Cl<sup>−</sup>cotransporter-mediated Cl<sup>−</sup> uptake. To directly test this possibility we studied the <i>in vivo</i> and vitro</i>functioning of acinar cells from parotid glands mice with targeted disruption Na<sup>+</sup>/K<sup>+</sup>/2Cl<sup>−</sup>cotransporter isoform 1 (<i>Nkcc1</i>), gene encoding Na<sup>+</sup>/K<sup>+</sup>/2Cl<sup>−</sup>cotransporter. In wild-type NKCC1 was localized...
SUMMARY The presence of copper‐binding proteins produced in response to added copper was examined isolates Laccaria laccata (Scop, ex Fr.) Cooke and Paxillus involutus (Batsch Fr. taken from copper‐contaminated uncontaminated sites, a single isolate Scleroderma citrinum Pers. contaminated site. Two (GLac4 ELacl) grew better 1–5 miu 2–5 niM than third (Lac3G) were considered be more tolerant. Amongst five P. , three (WJPax2R, GPaxRSp2 Pax4) capable growth media containing 4–0 mM regarded as...
The Bruce effect, the inhibition of pregnancy by a 'strange' male before implantation (Bruce, 1959), has been demonstrated in house mice, Mus musculus 1959; Chipman & Fox, 1966), prairie deermice, Peromyscus maniculatus bairdii (Eleftheriou, Bronson Zarrow, 1962), field voles, Microtus agrestis (Clulow Clarke, 1968), M. ochrogaster (Stehn Richmond, 1975), and meadow pennsylvanicus Langford, 1971). In their study Stehn Richmond (1975) described postimplantation disruption non-stud as late Day...
Multiple Na(+)/H(+) exchangers (NHEs) are expressed in salivary gland cells; however, their functions the secretion of saliva by acinar cells and subsequent modification ionic composition this fluid ducts unclear. Mice with targeted disruptions Nhe1, Nhe2, Nhe3 genes were used to study vivo these parotid glands. Immunohistochemistry indicated that NHE1 was localized basolateral NHE2 apical membranes both duct cells, whereas NHE3 restricted region cells. exchange reduced more than 95% greater...
Urea has environmental, agricultural and clinical importance being present in many bodily fluids including blood, urine, tears sweat.
The onset of salivary gland fluid secretion in response to muscarinic stimulation is accompanied by up-regulation Na(+)/H(+) exchanger (NHE) activity. Although multiple NHE isoforms (NHE1, NHE2, and NHE3) have been identified glands, little known about their specific function(s) resting secreting acinar cells. Mice with targeted disruptions the Nhe1, Nhe2, Nhe3 genes were used investigate contribution these proteins stimulation-induced activity mouse parotid lack NHE1, but not NHE2 or NHE3,...
1. The effects of fluid secretory stimuli on the Na(+)‐K(+)‐2Cl‐ cotransporter in rat parotid acini were investigated. Cotransporter activity was measured using NH4+ as a K+ surrogate and following cotransporter‐mediated fluxes by monitoring intracellular pH. 2. A dramatic upregulation (15‐ to 20‐fold) acinar induced muscarinic, alpha 1‐adrenergic peptidergic stimuli. half‐maximal effect muscarinic agonist carbachol observed at approximately 0.5 microM. 3. Our results indicate that rise...
1. We have studied the effects of acetylcholine (ACh) on fluid secretion and intracellular messengers in interlobular ducts isolated from rat pancreas maintained short‐term tissue culture. 2. Ductal was measured using micropuncture techniques. Intracellular free calcium ([Ca2+]i) cyclic AMP concentrations were single fura‐2 microspectrofluorimetry radioimmunoassay techniques respectively. Changes levels these correlated with secretion. 3. ACh stimulated ductal The dose required for a...
1. Interlobular ducts were isolated from the rat pancreas and maintained in short‐term tissue culture. Fluid secretion these was measured using micropuncture techniques, intracellular calcium concentration ([Ca2+]i) by fura‐2 microspectrofluorimetry, cyclic AMP radioimmunoassay. 2. Applying secretin ACh simultaneously to caused either a stimulation or an inhibition of fluid depending on doses employed. 3. The inhibitory effect could be relieved atropine, protein kinase C (PKC) inhibitors...
Fluid and exocrine secretion of mucins by salivary mucous glands is regulated predominantly parasympathetic activation muscarinic receptors. A direct role for subsequent putative signaling steps, phospholipase C (PLC), increased intracellular calcium ([Ca 2+ ] i ), isoforms protein kinase (PKC) in mediating has not been elucidated, these are potential therapeutic targets to enhance mucin hyposalivary patients. We found that muscarinic-induced rat sublingual tubulo-acini was dependent upon...