A5008879888- Genetics, Aging, and Longevity in Model Organisms
- Mitochondrial Function and Pathology
- FOXO transcription factor regulation
- Circadian rhythm and melatonin
- ATP Synthase and ATPases Research
- Aging and Gerontology Research
- RNA and protein synthesis mechanisms
- CRISPR and Genetic Engineering
- Vibrio bacteria research studies
- Salmonella and Campylobacter epidemiology
- Diamond and Carbon-based Materials Research
- Heat shock proteins research
- Pain Management and Treatment
- Bacterial Genetics and Biotechnology
- Fullerene Chemistry and Applications
- Selenium in Biological Systems
- Urinary Bladder and Prostate Research
- Boron and Carbon Nanomaterials Research
- Botulinum Toxin and Related Neurological Disorders
- RNA Research and Splicing
Villanova University
2016-2022
Massachusetts Institute of Technology
2008-2011
Johns Hopkins University
2004-2009
Johns Hopkins Medicine
2004-2007
Pepperdine University
1999
The decline in immune function with aging, known as immunosenescence, has been implicated evolutionarily diverse species, but the underlying molecular mechanisms are not understood. During aging Caenorhabditis elegans, intestinal tissue deterioration and increased proliferation of bacteria observed, how innate immunity changes during C. elegans defined. Here we show that exhibits susceptibility to bacterial infection age, establish is associated a activity conserved PMK-1 p38...
The mitochondrial outer membrane protein, Mmm1p, is required for normal shape in yeast. To identify new morphology proteins, we isolated mutations incompatible with the mmm1-1 mutant. One of these mutants, mmm2-1, defective a novel protein. Lack Mmm2p causes defect and loss DNA (mtDNA) nucleoids. Like Mmm1 protein (Aiken Hobbs, A.E., M. Srinivasan, J.M. McCaffery, R.E. Jensen. 2001. J. Cell Biol. 152:401–410.), located dot-like particles on surface, many which are adjacent to mtDNA While...
We previously discovered several phosphorylations to the beta subunit of mitochondrial F(1)F(o) ATP synthase complex in isolated rabbit myocytes on adenosine treatment, an agent that induces cardioprotection. The role these is unknown.The present study focuses functional consequences phosphorylation by generating nonphosphorylatable and phosphomimetic analogs a model system, Saccharomyces cerevisiae.The 4 amino acid residues with homology yeast (T58, S213, T262, T318) were studied respect...
Aging is accompanied by a progressive decline in immune function termed "immunosenescence". Deficient surveillance coupled with the impaired of cells compromises host defense older animals. The dynamic activity regulatory modules that control immunity appears to underlie age-dependent modifications system. In roundworm Caenorhabditis elegans levels PMK-1 p38 MAP kinase diminish over time, reducing expression effectors clear bacterial pathogens. Along pathway, innate C. regulated insulin...
The iprA gene (formerly known as yaiV or STM0374) is located in a two-gene operon the Salmonella enterica serovar Typhimurium genome and associated with altered expression during spaceflight rotating-wall-vessel culture conditions that increase virulence. However, uncharacterized literature. In this report, we present first targeted characterization of gene, which revealed highly conserved across Enterobacteriaceae We found S Typhimurium, Escherichia coli, Enterobacter cloacae ΔiprA mutant...
The insulin and insulin-like growth factor signaling (IIS) pathway regulates stress resistance contributes to longevity in evolutionarily diverse species. In the roundworm Caenorhabditis elegans lifespan of daf-2 mutants, which lack functional receptors, is twice that wildtype animals. This phenotype entirely dependent upon FOXO transcription DAF-16. absence negative regulation through IIS pathway, DAF-16 upregulates genes important for resistance, defense, repair. While it can be activated...
ABSTRACT Aging is accompanied by a progressive decline in immune function termed “immunosenescence”. Deficient surveillance coupled with the impaired of cells compromises host defense older animals. The dynamic activity regulatory modules that control immunity appears to underlie age-dependent modifications system. In roundworm Caenorhabditis elegans levels PMK-1 p38 MAP kinase diminish over time, reducing expression effectors clear bacterial pathogens. Along pathway, innate C. regulated...
Abstract Insulin and insulin-like growth factors are longevity determinants that negatively regulate Forkhead box class O (FoxO) transcription factors. In C. elegans mutations constitutively activate DAF-16, the ortholog of mammalian FoxO3a, extend lifespan by two-fold. While environmental insults induce DAF-16 activity in younger animals, it also becomes activated an age-dependent manner absence stress, modulating gene expression well into late adulthood. The mechanism which is regulated...
Insulin and insulin-like growth factors are longevity determinants that negatively regulate Forkhead box class O (FoxO) transcription factors. In C . elegans mutations constitutively activate DAF-16, the ortholog of mammalian FoxO3a, extend lifespan by two-fold. While environmental insults induce DAF-16 activity in younger animals, it also becomes activated an age-dependent manner absence stress, modulating gene expression well into late adulthood. The mechanism which is regulated during...
ABSTRACT The insulin/insulin-like growth factor signaling (IIS) pathway modulates growth, survival, and lifespan by regulating FOXO transcription factors. In Caenorhabditis elegans , IIS maintains DAF-16/FOXO in an inactive state unless animals are challenged environmental stress. Recent evidence suggests that DAF-16 becomes activated as part of normal aging C. yet the regulatory module responsible for this phenomenon is largely undefined. Embedded within phospholipid which PIP 3 produced...
A gradual breakdown in the cellular networks that assure protein stability, termed proteostasis, contributes significantly to accumulation of misfolded and aggregated proteins severely compromises function later life. progressive decline efficiency unfolded response (UPR) endoplasmic reticulum (ER) causes cells become particularly susceptible disturbances affecting biogenesis, folding, turnover membrane proteins. While dynamics ER stress mechanisms during aging have been well-characterized,...