A5022120995- Biochemical Analysis and Sensing Techniques
- Regulation of Appetite and Obesity
- Olfactory and Sensory Function Studies
- Advanced Chemical Sensor Technologies
- Neuroscience and Neuropharmacology Research
- Ion channel regulation and function
- Adipose Tissue and Metabolism
- Neurotransmitter Receptor Influence on Behavior
- Adipokines, Inflammation, and Metabolic Diseases
- Family and Disability Support Research
- Analytical Chemistry and Chromatography
- melanin and skin pigmentation
- Cardiac electrophysiology and arrhythmias
- Bone Metabolism and Diseases
- Bone health and treatments
- Down syndrome and intellectual disability research
- Color perception and design
- Tryptophan and brain disorders
- Bone health and osteoporosis research
- Autism Spectrum Disorder Research
- Analytical Chemistry and Sensors
- Neuroscience and Neural Engineering
University of Michigan
2023-2024
Laboratoire d’immunologie intégrative du cancer
2024
The University of Texas Southwestern Medical Center
2024
University of Central Florida
2019-2023
Michigan United
2023
Bowling Green State University
2014
The melanocortin-3 receptor (MC3R) is a negative regulator of the central melanocortin circuitry via presynaptic expression on agouti-related protein (AgRP) nerve terminals, from where it regulates GABA release onto secondary MC4R-expressing neurons. However, MC3R knockout (KO) mice also exhibit defective behavioral and neuroendocrine responses to fasting. Here, we demonstrate that KO activation AgRP neurons in response fasting, cold exposure, or ghrelin while exhibiting normal inhibition by...
The melanocortin-3 receptor (MC3R) regulates GABA release from agouti-related protein (AgRP) nerve terminals and thus tonically suppresses multiple circuits involved in feeding behavior energy homeostasis. Here, we examined the role of MC3R melanocortin system regulating response to various anorexigenic agents. genetic deletion or pharmacological inhibition MC3R, subthreshold doses an MC4R agonist, improved dose responsiveness glucagon-like peptide 1 (GLP1) agonists, as assayed by food...
Melanocortin 4 receptor (MC4-R) antagonists are actively sought for treating cancer cachexia. We determined the structures of complexes with PG-934 and SBL-MC-31. These peptides differ from SHU9119 by substituting His6 Pro6 inserting Gly10 or Arg10. The revealed two subpockets at TM7-TM1-TM2 domains, separated N2857.36. Two peptide series based on complexed led to an antagonist activity selectivity SAR study. Most ligands retained potency, but several SBL-MC-31-derived significantly enhanced...
Using molecular, cellular, and behavioral analyses, this study shows that sex differences occur in fat taste a mouse model. Female mice are more responsive to fatty acids, leading an overall decrease intake acid preference. These linked hormones, as estradiol enhances cell responsiveness acids during periods of low circulating estrogen following ovariectomy males. Estradiol is ineffective altering signaling high-estrogen period ovariectomized on hormone replacement. Thus, receptor cells...
Adiponectin, a key metabolic hormone, is secreted into the circulation by fat cells where it enhances insulin sensitivity and stimulates glucose fatty acid metabolism. Adiponectin receptors are highly expressed in taste system; however, their effects mechanisms of action modulation gustatory function remain unclear. We utilized an immortalized human fungiform cell line (HuFF) to investigate effect AdipoRon, adiponectin receptor agonist, on acid-induced calcium responses. showed that (CD36...
Few studies have investigated the effect of a monosaturated diet high in ω-9 on osteoporosis. We hypothesized that omega-9 (ω-9) protects ovariectomized (OVX) mice from decline bone microarchitecture, tissue loss, and mechanical strength, thereby serving as modifiable dietary intervention against osteoporotic deterioration. Female C57BL/6J were assigned to sham-ovariectomy, ovariectomy, or ovariectomy + estradiol treatment prior switching their feed for 12 weeks. Tibiae evaluated using DMA,...
The ability of mammalian taste cells to respond fatty acids (FAs) has garnered significant attention late and been proposed represent a sixth primary taste. With few exceptions, studies on FA have centered exclusively polyunsaturated FAs, most notably linoleic acid. In the current study, we identified an additional receptor, GPR84, in gustatory system that responds medium-chain saturated FAs (MCFAs) male mice. GPR84 ligands activate both Type II III calcium imaging patch-clamp recording...
Most antipsychotic drugs (APDs) induce hyperphagia and weight gain. However, the neural mechanisms are poorly understood, partly due to challenges replicating their metabolic effects in rodents. Here, we report a new mouse model that recapitulates overeating induced by clozapine, widely prescribed APD. Our study shows clozapine boosts food intake inhibiting melanocortin 4 receptor (MC4R) expressing neurons paraventricular nucleus of hypothalamus. Interestingly, neither nor risperidone,...
ABSTRACT The melanocortin‐3 receptor (MC3R) is a G‐protein coupled that regulates appetite and potential therapeutic target for anorexia weight loss treatments. report of highly selective synthetic antagonist MC3R, Cpd11, represents major advance toward targeting MC3R. However, Cpd11 challenging to access synthetically, severely limiting its use additional structural optimization. Here, we outline an improved synthesis addresses three challenges, including the formation Cpd11's structurally...
Ghrelin is a major appetite-stimulating neuropeptide found in circulation. While its role increasing food intake well known, affecting taste perception, if any, remains unclear. In this study, we investigated the of growth hormone secretagogue receptor’s (GHS-R; ghrelin receptor) activity peripheral system using feeding studies and conditioned aversion assays by comparing wild-type GHS-R-knockout models. Using transgenic mice expressing enhanced green fluorescent protein (GFP), demonstrated...
Abstract The melanocortin-3 receptor (MC3R) acts presynaptically to regulate GABA release from agouti-related protein (AgRP) nerve terminals and thus may be a negative regulator of multiple circuits involved in feeding behavior energy homeostasis. Here, we examined the role MC3R regulating response various anorexigenic agents. Our findings reveal that genetic deletion or pharmacological inhibition improves dose responsiveness Glucagon-like peptide 1 (GLP1) agonists, as assayed by food intake...
Hereditary defects in the function of Kir7.1 retinal pigment epithelium are associated with ocular diseases retinitis pigmentosa, Leber congenital amaurosis, and snowflake vitreal degeneration. Studies also suggest that may be regulated by a GPCR, melanocortin-4 receptor, certain hypothalamic neurons. We present first structures human describe conformational bias displayed two pathogenic mutations, R162Q E276A, to provide an explanation for basis disease illuminate gating pathway....
Purpose Individuals with autism spectrum disorder (ASD) often receive services from a variety of professionals. However, not all providers adequate training in ASD. The Leadership Education Neurodevelopmental and Related Disabilities (LEND) program includes core competency increasing knowledge about neurodevelopmental related disabilities. This study attempted to assess trainees’ ASD self-reported confidence working individuals sought understand if through the LEND increases these...
Transient Receptor Potential channel type Melastatin 5 (TRPM5) is a nonselective cation that activated by an increase in cytosolic calcium. TRPM5 channels are highly expressed Type II taste receptor cells transduce bitter, sweet, umami, and fatty acid compounds. While the function of well‐defined these cells, effects hormones such as estrogen on TRPM5‐mediated signaling impact system not well known. We investigated activation acid‐sensing physiological long‐term fat intake males females....
The melanocortin-3 receptor (MC3R) is a negative regulator of the central melanocortin circuitry via presynaptic expression on AgRP nerve terminals, from where it regulates GABA release onto secondary MC4R-expressing neurons. Hence, animals lacking MC3R (MC3R KO) exhibit hypersensitivity to MC4R agonists. However, KO mice also defective behavioral and neuroendocrine responses fasting. Here, we demonstrate that activation neurons in response fasting cold exposure, while exhibiting normal...