Fragile X syndrome generally arises as a consequence of large expansion CGG trinucleotide repeat element that is located in the GC-rich promoter region fragile mental retardation gene (FMR1). In conventional model for X, clinical involvement silencing FMR1 gene, with attendant loss protein (FMRP). However, it has recently been demonstrated most males premutation alleles (100–200 repeats), or unmethylated full mutation alleles, have mRNA levels are higher than normal, despite reduced FMRP....

Purpose To screen for subclinical keratoconus by analyzing corneal, epithelial, and stromal thickness map patterns with Fourier-domain optical coherence tomography (OCT). Setting Four centers in the United States. Design Cross-sectional observational study. Methods Eyes of normal subjects, eyes, topographically eye a unilateral patient were studied. Corneas scanned using 26 000 Hz OCT system (RTVue). Normal subjects divided into training evaluation groups. Corneal, maps derived diagnostic...

Electronic health record (EHR) systems have transformed the practice of medicine. However, physicians raised concerns that EHR time requirements negatively affected their productivity. Meanwhile, evolving approaches toward physician reimbursement will require additional documentation to measure quality and cost care. To date, little quantitative analysis has rigorously studied these topics.To examine ophthalmologist for use.A single-center cohort study was conducted between September 1,...

Purpose: To improve accuracy and efficiency in quantifying the endothelial cell loss (ECL) eye bank preparation of corneal grafts.

Abstract Objective Outpatient clinics lack guidance for tackling modern efficiency and productivity demands. Workflow studies require large amounts of timing data that are prohibitively expensive to collect through observation or tracking devices. Electronic health records (EHRs) contain a vast amount – timestamps collected during regular use can be mapped workflow steps. This study validates using EHR timestamp predict outpatient ophthalmology clinic timings at Oregon Health Science...

Purpose To distinguish between corneal ectasia and contact lens–related warpage by characteristic patterns on topography optical coherence tomography (OCT) epithelial thickness maps. Setting Casey Eye Institute, Portland, Oregon, USA. Design Prospective retrospective case series. Methods Axial mean power maps were obtained systems. Epithelial generated using RTVue OCT. A sector divider was applied to all The locations of the minimum thickness, maximum axial power, determined based averages....

A consecutive case series of patients with dupilumab-associated ocular surface disease (DAOSD) that describes common symptoms and signs, proposes a symptom severity grading system, treatment strategies DAOSD was evaluated.A retrospective chart review concomitant dupilumab-treated atopic dermatitis ophthalmic evaluation between January 2014 May 2019 conducted.Twenty-nine (mean age 46 years, M/F: 12/17) 57 exams were identified. The most included irritation/pain (n = 28, 97%), redness 24,...

Purpose: To train and validate a convolutional neural network (CNN) to detect the history of laser-assisted in situ keratomileusis (LASIK) surgeries using corneal optical coherence tomography (OCT) maps. Methods: Five OCT maps (pachymetry, epithelial thickness, posterior mean curvature, anterior axial power, stroma reflectance) were utilized as input lightweight CNN model. scans healthy volunteers patients who had undergone myopic or hyperopic LASIK included. Repeated fivefold...

Purpose: To detect keratoconus using optical coherence tomography (OCT) corneal map parameters and patterns. Setting: Casey Eye Institute, Oregon Health Science University, Portland, Oregon. Design: Cross-sectional observational study. Methods: A spectral-domain OCT was used to acquire epithelial thickness maps in normal, manifest keratoconic, subclinical forme fruste keratoconic (FFK) eyes. 2-step decision tree designed. An eye will be classified as if both conditions are met. First, at...

Purpose: The aim of this study was to describe a case corneal involvement as an early manifestation ocular disease in the 2022 human mpox (monkeypox) virus outbreak. Methods: This is single report with longitudinal care. Results: A 47-year-old immunocompetent man presented viral conjunctivitis before development skin lesions or systemic symptoms. Subsequently, he developed membranous keratoconjunctivitis and epithelial defect. Orthopoxvirus-positive polymerase chain reaction test from his...

Both topical ocular and intranasal immunizations have been reported to stimulate the mucosal immune system (OMIS) systemic system. Nasolacrimal ducts (NLDs) are connecting bridges between OMIS nasal cavity-associated lymphoid tissue (NALT). These drain ocularly administrated solutions into inferior meatus of nose reach NALT. Inversely, NLDs also intranasally surface eye thus OMIS. This unique anatomical connection NALT systems provoked us test whether immunologically interdependent. In this...

To evaluate and compare corneal higher-order aberrations (HOAs) after Descemet stripping automated endothelial keratoplasty (DSAEK), femtosecond laser-assisted penetrating (FLAK), conventional (PKP).A retrospective comparison of consecutive surgical series 67 eyes 59 patients between 1.5 19 months transplant surgery (22, 34, 11 corneas underwent DSAEK, FLAK, PKP, respectively, by a single surgeon). The main outcome measures were anterior posterior surface HOAs (Zernike polynomials, third to...

To differentiate between keratoconus and contact lens-related corneal warpage by combining focal change patterns in anterior topography, pachymetry, epithelial thickness maps.Pachymetry maps of normal, keratoconus, warpage, forme fruste (FFK) eyes were obtained from a Fourier-domain optical coherence tomography (OCT). Epithelial pattern standard deviation (PSD) was calculated combined with two novel indices, the Warpage Index Anterior Ectasia Index, to eyes. The values three parameters...

Purpose: To evaluate endothelial cell damage after eye bank preparation and passage through 1 of 2 different injectors for Descemet membrane keratoplasty grafts. Methods: Eighteen grafts were prepared by Lions VisionGift with the standard partial prepeel technique placement an S-stamp orientation. The randomly assigned to injection either a glass-modified Jones tube injector (Gunther Weiss Scientific Glass) or closed-system intraocular lens (Viscoject 2.2; Medicel). After injection, stained...

In normally growing Drosophila cultured cells the heat shock transcription factor (dHSF) is localized in cytosol and translocates into nucleus after shock. of nonshocked cells, dHSF present as a monomer that cannot bind DNA. Upon stress, enters where it observed to be trimer. A novel nuclear localization sequence (NLS) was found responsible for stress-dependent entry. Deletion NLS prevents entry, expected, yet surprisingly also allows constitutive oligomerization DNA binding cytosol. Further...

<h3>Purpose</h3> To evaluate wound strength for patient safety during transport and endothelial viability when partial complete femtosecond laser-assisted keratoplasty (FLAK) incisions are made in cadaveric corneas. <h3>Methods</h3> 19 human corneoscleral rims were divided into six groups, mounted on an anterior chamber maintainer cut with a laser programmed to the following patterns: 'zigzag' (A), 'mushroom' (B) 'top hat' (C) both full (1) (2) thicknesses. The pressure required produce...

Vision-related quality of life can be a valuable outcome for some interventions in ophthalmology. In the primary Descemet Endothelial Thickness Comparison Trial (DETECT), membrane endothelial keratoplasty (DMEK) had superior postoperative visual acuity compared with ultrathin stripping automated (UT-DSAEK). It is interest to determine whether this trend extends life.To effect UT-DSAEK and DMEK on vision-related life.A prespecified secondary analysis 2-surgeon patient- outcome-masked...