A5019376982- Marine Toxins and Detection Methods
- Microbial Community Ecology and Physiology
- Marine and coastal ecosystems
- Diatoms and Algae Research
- Genomics and Phylogenetic Studies
- Protist diversity and phylogeny
- Identification and Quantification in Food
- Analytical chemistry methods development
- Marine Biology and Ecology Research
- Coral and Marine Ecosystems Studies
- Marine Bivalve and Aquaculture Studies
- Aquatic Ecosystems and Phytoplankton Dynamics
- Plant Pathogens and Fungal Diseases
- Rangeland Management and Livestock Ecology
- Plant Diversity and Evolution
- Protein purification and stability
- Industrial Technology and Control Systems
- Plant Toxicity and Pharmacological Properties
- Genetic and phenotypic traits in livestock
- Environmental Toxicology and Ecotoxicology
- Innovative Microfluidic and Catalytic Techniques Innovation
- Environmental Chemistry and Analysis
- Advanced Algorithms and Applications
- Mass Spectrometry Techniques and Applications
- Vibrio bacteria research studies
Universiti Malaysia Sarawak
2011-2024
Tunku Abdul Rahman University of Management and Technology
2015-2023
Forest Department Sarawak
2020
University of Malaya
2014-2015
Purdue University West Lafayette
1987
Field sampling was undertaken to investigate the occurrence of Pseudo-nitzschia Peragallo species in eight locations along coast Malaysian Borneo. A total 108 strains were isolated, and their morphology examined with SEM TEM. Additionally, molecular data from nuclear-encoded partial LSU rDNA, ITS regions, characterized. five confidently identified based on a combination distinct morphological characteristics supporting evidence: P. brasiliana Lundholm, Hasle & Fryxell, cuspidata (Hasle)...
A study on the morphology and phylogeny of 18 strains Pseudo-nitzschia established from Strait Malacca, Peninsular Malaysia, was undertaken. Morphological data combined with molecular evidence show that they constitute three new species, for which names, P. batesiana sp. nov., lundholmiae fukuyoi are proposed. The species closely resemble in pseudodelicatissima complex sensu lato. Morphologically, differs other by having a smaller part cell overlapping chain, whereas fewer poroid sectors...
Forty-eight isolates of Pseudo-nitzschia species were established from the Miri coast Sarawak (Malaysian Borneo) and underwent TEM observation molecular characterization. Ten found: P. abrensis, batesiana, fukuyoi, kodamae, lundholmiae, multistriata, pungens, subfraudulenta, as well two additional new morphotypes, herein designated bipertita sp. nov. limii This is first report lundholmiae in coastal waters Malaysian Borneo. differs its congeners by number sectors that divide poroids,...
Analyses of the mitochondrial cox1 , nuclear‐encoded large subunit ( LSU ), and internal transcribed spacer 2 ITS 2) RNA coding region Pseudo‐nitzschia revealed that P. pseudodelicatissima complex can be phylogenetically grouped into three distinct clades (Groups I–III), while delicatissima forms another clade (Group IV) in both phylogenetic trees. It was elucidated comprehensive taxon sampling (sampling sequences), selection appropriate target genes outgroup, alignment strategies influenced...
A new species of Pseudo-nitzschia (Bacillariophyceae) is described from plankton samples collected Port Dickson (Malacca Strait, Malaysia) and Manzanillo Bay (Colima, Mexico). The possesses a distinctive falcate cell valve, which they form sickle-like colonies in both environmental cultured strains. Detailed observation frustules under TEM revealed ultrastructure that closely resembles P. decipiens, yet the differs by valve shape greater ranges striae poroid densities. readily distinguished...
Abstract Species of the diatom Pseudo-nitzschia are known to produce domoic acid that is responsible for amnesic shellfish poisoning (ASP). To investigate potential risk ASP and species occurrence toxic or potentially in Malaysian waters, plankton samples were collected from 17 locations. Samples examined through transmission electron microscopy. identified on basis frustule morphology morphometric measurements. Twenty-two well-described recorded, which 14 new records Malaysia. A morphotype,...
:We explored the 18S rDNA sequences of Pseudo-nitzschia and its close relatives, together with their individual secondary structure information to reconstruct phylogenies. Forty-eight taxa classified in Bacillariaceae Achnanthaceae were analyzed by character-based (maximum parsimony maximum likelihood) distance-based (neighbour-joining) methods. Three raphid diatoms from Eunotiaceae used as out-groups. The sequence-structure alignment was generated 4SALE (i.e. a one-letter encoded that...
Abstract Different clades belonging to the cosmopolitan marine diatom Pseudo-nitzschia pungens appear be present in different oceanic environments, however, a ‘hybrid zone’, where populations of interbreed, has also been reported. Many studies have investigated sexual reproduction P. pungens, focused on morphology and life cycle, rather than role mixing genomes their parents. We carried out crossing experiments determine compatibility/incompatibility between , examined genetic polymorphism...
SUMMARY Certain species of the marine diatom genus Pseudo‐nitzschia are responsible for production domoic acid (DA), a neurotoxin that can bioaccumulate in food chain and cause amnesic shellfish poisoning (ASP) animals humans. This study extends our knowledge by reporting on first observation potentially toxic simulans from this region. One clonal strain P. was isolated East Australian Current characterized using light transmission electron microscopy, phylogenetic analyses based regions...
Abstract With the increasing demand for giant clams in ornamental trade as well habitat destruction of coral reefs, clam populations have been threatened across Indo‐Pacific. This study documents population status and genetic diversity Perhentian Islands Marine Park (PMP), a Protected Area (MPA) on east coast Peninsular Malaysia, plus Rhu Island, an adjacent island outside MPA. Of 13 reef sites surveyed area 11,200 m 2 , two species were recorded: Tridacna squamosa maxima with average...
SUMMARY We investigated a harmful algal bloom ( HAB ) associated with the massive fish kills in J ohor S trait, M alaysia, which recurred year after first incident 2014. This has urged for need to have rapid and precise method monitoring. In this study, we develop SYBR green‐based real‐time PCR (q detect culpable dinoflagellate species, Karlodinium australe . Species‐specific q primers were designed gene region of second internal transcribed spacer ribosomal RNA (r DNA ). The species...
A field survey was carried out in Aman Island, the northern Strait of Malacca, to investigate occurrence harmful microalgae. The area is known for marine finfish cage-culture and blood cockle farming. Plankton samples were collected fortnightly between July 2011 September 2013. total 58 phytoplankton genera recorded; 37 belong diatoms, 21 dinoflagellates. Harmful microalgae species identified microscopically, which nine are bloom-forming that responsible fish kills. These included Akashiwo...