A5019279284- Chromosomal and Genetic Variations
- DNA Repair Mechanisms
- Genomics and Chromatin Dynamics
- Carcinogens and Genotoxicity Assessment
- PARP inhibition in cancer therapy
- Genetic and Clinical Aspects of Sex Determination and Chromosomal Abnormalities
- Plant Genetic and Mutation Studies
- Developmental Biology and Gene Regulation
University of Michigan
2022-2024
Georgetown University
2016
Georgetown University Medical Center
2016
Aging is characterized by genome instability, which contributes to cancer formation and cell lethality leading organismal decline. The high levels of DNA double-strand breaks (DSBs) observed in old cells premature aging syndromes are likely a primary source but the underlying cause their still unclear. DSBs might result from higher damage or repair defects emerging with advancing age, pathways organisms poorly understood. Here, we show that premeiotic germline young flies have distinct...
DNA double-strand breaks (DSBs) are a particularly deleterious class of damage that threatens genome integrity. DSBs repaired by three pathways: nonhomologous-end joining (NHEJ), homologous recombination (HR), and single-strand annealing (SSA). Drosophila melanogaster Blm (DmBlm) is the ortholog Saccharomyces cerevisiae SGS1 human BLM, has been shown to suppress crossovers in mitotic cells repair gaps via HR. To further elucidate role DmBlm simple DSB, particular mechanisms, we utilized...
Phenotypic evolution is often caused by variation in gene expression resulting from altered regulatory mechanisms. Genetic affecting chromatin remodeling has been identified as a potential source of variable expression; however, the roles specific factors remain unclear. Here, we address this knowledge gap examining relationship between expression, structure, and binding pioneer factor Grainy head imaginal wing discs two divergent strains Drosophila melanogaster their F1 hybrid. We find that...
Pioneer factors are critical for gene regulation and development because they bind chromatin make DNA more accessible binding by other transcription factors. The pioneer factor Grainy head (Grh) is present across metazoans has been shown to retain a role in epithelium fruit flies, nematodes, mice despite extensive divergence both amino acid sequence length. Here, we investigate the evolution of Grh function comparing effects fly (