Membrane depolarization activates voltage-dependent Ca 2+ channels (VDCCs) inducing release via ryanodine receptors (RyRs), which is obligatory for skeletal and cardiac muscle contraction other physiological responses. However, depolarization-induced its functional importance as well underlying signaling mechanisms in smooth cells (SMCs) are largely unknown. Here we report that membrane can induce RyR-mediated local release, leading to a significant increase the activity of sparks airway...

Here we attempted to test a novel hypothesis that hypoxia may induce Ca(2+) release through reactive oxygen species (ROS)-mediated dissociation of FK506-binding protein 12.6 (FKBP12.6) from ryanodine receptors (RyRs) on the sarcoplasmic reticulum (SR) in pulmonary artery smooth muscle cells (PASMCs). The results reveal hypoxic exposure significantly decreased amount FKBP12.6 SR PAs and increased cytosol. colocalization with RyRs was intact PASMCs. Pharmacological genetic inhibition...

Hypoxia-induced pulmonary vasoconstriction (HPV) is attributed to an increase in intracellular Ca2+ concentration ([Ca2+]i) artery smooth muscle cells (PASMCs). We have reported that phospholipase C-γ1 (PLCγ1) plays a significant role the hypoxia-induced [Ca2+]i PASMCs and attendant HPV. In this study, we intended determine molecular mechanisms for hypoxic contractile responses PASMCs. Our data reveal occurs arteries, but not mesenteric arteries. Hypoxia caused large PASMCs, which diminished...

Ca

An increase in intracellular calcium concentration ([Ca 2+ ] i ) pulmonary arterial smooth muscle cells (PASMCs) induces hypoxic cellular responses the lungs; however, underlying molecular mechanisms remain incompletely understood. We report, for first time, that acute hypoxia significantly enhances phospholipase C (PLC) activity mouse resistance arteries (PAs), but not mesenteric arteries. Western blot analysis and immunofluorescence staining reveal expression of PLC-γ1 protein PAs PASMCs,...

Hypoxia causes heterogeneous contractile responses in resistance and conduit pulmonary as well systemic (mesenteric) artery smooth muscle cells (RPASMCs, CPASMCs MASMCs), but the underlying mechanisms are largely unknown. In this study, we aimed to investigate whether gene expression functional activity of ryanodine receptors (RyRs) would be different these 3 cell types.RyR mRNA expression, Ca(2+) sparks [Ca(2+)](i) were measured by real-time quantitative RT-PCR, laser scanning confocal...

Background and Purpose Stimulation of the A 1 adenosine receptor angiotensin II type‐1 (AT receptor) causes vasoconstriction through activation cytochrome P450 4A (CYP4A) ERK1/2. Thus, we hypothesized that acute alters vasomotor response induced by non‐selective agonist, NECA, in mouse mesenteric arteries (MAs). Experimental Approach We used a Danish Myo Technology wire myograph to measure muscle tension isolated MAs from wild type (WT), 2B knockout (KO) mice. Western blots were performed...

Abstract OBJECTIVE We previously showed that uridine adenosine tetraphosphate (Up4A)-mediated aortic contraction is partly mediated through purinergic P2X1 receptors (P2X1R). It has been reported the plasma level of Up4A elevated in hypertensive patients, implying a potential role for Up4A-P2X1R signaling hypertension. This study investigated vasoactive effect aortas isolated from angiotensin (Ang) II-infused (21 days) mice. METHODS Blood pressure was measured by tail cuff plethysmography....

Dandruff is a common scalp condition characterized by excessive scaling and itch. Aberrant colonization of the commensal Malassezia spp. major contributor in multifactorial etiology dandruff. Literature based understanding linked pathophysiology dandruff allowed us to comprehend strategy potentiate efficacy known antifungal agent used therapy. The aim this study was determine skin safety VB-001 antidandruff leave-on formulation comparison with marketed ZPTO shampoo patients moderate adherent...

Local Ca 2+ signals (Ca sparks) play an important role in multiple cellular functions airway smooth muscle cells (ASMCs). Protein kinase Cϵ is known to downregulate ASMC sparks and contraction; however, no complementary phosphatase has been shown produce opposite effects. Here, we for the first time report that treatment with a specific calcineurin (CaN) autoinhibitory peptide (CAIP) block CaN activity decreases, whereas application of nickel activate increases, both presence absence...

The present study was conducted on advanced pregnant buffaloes suffering from uterine torsion to assess the status of fetus and uterus by transabdominal ultrasonography, findings were compared with normal buffaloes.The 20 clinical cases (control group). lower ventral area just lateral linea alba (on both sides udder) in standing animals scanned transabdominally two-dimensional convex transducer for various ultrasonographic findings. data collected statistically analyzed "one-way ANOVA"...

Background: L-carnitine (LC) might have the potential to control inflammation by reduction of major inflammatory cytokines, including CRP. Therefore, this study was planned investigate effect LC supplementation on CRP levels in CAD patients.Methods: 50 subjects comprised placebo group who received standard line treatment for coronary artery disease while other 1000 mg daily apart from 12 weeks. were measured at baseline and again after weeks all subjects.Results: The participants had...

Uninhibited proliferation of Malassezia spp., enhanced sebaceous gland activity and individual sensitivity are three prime etiological factors behind dandruff. For many dandruff sufferers, existing anti-dandruff products start yielding unsatisfactory results after a few cycles use. This observation made us explore the physical biological environment host exploit specific type lipid dependence spp. for their survival. A shampoo formulation (product code VB-3222) was developed to address...

Resumo As lesões do tipo joelho flutuante (FKIs, na sigla em inglês) são complexas e geralmente causadas por trauma de alta velocidade. Estas frequentemente associadas a que causam risco morte, devem ter precedente sobre nas extremidades. Os autores revisaram os resultados das tratadas nesta instituição entre 2003 2015. Foi realizado um estudo retrospectivo todos pacientes com FKIs Doze foram incluídos no estudo. dados relacionados ao fratura, associadas, modalidades tratamento complicações...

Hypoxia is well known to cause pulmonary vasoconstriction, but usually does not induce systemic (e.g., mesenteric) vasoconstriction. The molecular mechanisms responsible for the heterogeneous hypoxic responses in these two different vascular systems remain unclear. Herein we provide data showing that mRNA and protein expression levels of Rieske iron-sulfur (RISP) mitochondrial complex III are significantly higher artery smooth muscle cells (PASMCs) than mesenteric SMCs (MASMCs). Exposure...

Background: Hypertensive retinopathy (HR) represents the ophthalmic findings of end-organ damage secondary to systemic arterial hypertension. Platelets play a crucial role in pathogenesis atherosclerotic complications, contributing thrombus formation or apposition after plaque rupture. The aim our study was investigate whether Mean platelet volume (MPV) is associated with severity hypertensive patients.Methods: This cross-sectional conducted Department Medicine SAMC and PGI, Indore. Total...