A5077752545- Trace Elements in Health
- Heavy Metal Exposure and Toxicity
- Viral Infectious Diseases and Gene Expression in Insects
- Protein purification and stability
- Cystic Fibrosis Research Advances
- Iron Metabolism and Disorders
- Cancer Cells and Metastasis
- Lung Cancer Treatments and Mutations
- Neonatal Respiratory Health Research
- Cancer Treatment and Pharmacology
- MicroRNA in disease regulation
- Cancer therapeutics and mechanisms
- Immune Response and Inflammation
- Animal Genetics and Reproduction
- Protease and Inhibitor Mechanisms
- Neonatal and Maternal Infections
- Muscle metabolism and nutrition
- RNA Interference and Gene Delivery
- Aluminum toxicity and tolerance in plants and animals
- Medicinal Plant Pharmacodynamics Research
- Cancer-related gene regulation
- Redox biology and oxidative stress
- Endoplasmic Reticulum Stress and Disease
- Molecular Biology Techniques and Applications
- Insect symbiosis and bacterial influences
Dublin City University
2012-2024
University of Limerick
2024
Alltech (Ireland)
2017
National Institute for Bioprocessing Research and Training
2017
The ability of mammalian cell lines to sustain specific productivity (Qp) over the full duration bioprocess culture is a highly desirable phenotype, but molecular basis for sustainable has not been previously investigated in detail. In order identify proteins that may be associated with sustained we have conducted proteomic profiling analysis two matched pairs monoclonal antibody-producing Chinese hamster ovary (CHO) differ their 10 day fed-batch culture.Proteomic inherent differences...
Alterations in protein expression associated with adriamycin resistance a panel of variants derived from the poorly differentiated squamous cell lung carcinoma DLKP were investigated using 2-D DIGE. Of 80 proteins identified as being differentially expressed, 32 correlated to resistance. Twenty-four showed positive correlations drug resistance, 11 directly increase (including NDPK, RPA2, CCT2, HSP70 and Annexin A1) while 13 HNRP K H1, aldehyde dehydrogenase (ALDH), stomatin CCT3) increased...
Abstract BACKGROUND Radical prostatectomy cures the majority of men with clinically localized disease, but up to 30% relapse rising serum PSA levels. Stage, Gleason grade, and pre‐operative levels are associated outcome do not accurately predict which individuals will relapse. MicroRNA (miRNA) altered in cancer progression disease. The miR‐200 family has roles prostate cancer. METHODS miR‐200a were measured 18 radical samples from who did relapse, matched for stage (all T3), A pair normal...
Doxorubicin- and paclitaxel-selected variants of an in vitro invasive clonal population the human breast cancer cell line, MDA-MB-435S, were established by pulse selection, exhibited a novel 'superinvasive' phenotype. This phenotype is characterised ability to relocate another surface following invasion through matrigel membrane pores, decreased adhesion extracellular matrix proteins increased motility. may represent model step metastatic process occurring subsequent invasion. The...
Alpha-1 antitrypsin (AAT) is the major physiological inhibitor of a range serine proteases, and in lung, it maintains protease–antiprotease balance. AAT deficiency (AATD) an autosomal co-dominant condition with Z mutation being most common cause. Individuals homozygous for (PiZZ) have low levels circulating mutant Z-AAT protein leading to premature emphysematous lung disease. Extensive glycoanalysis has been performed on normal (M-AAT) from healthy individuals importance glycosylation...
Alpha-1 antitrypsin (AAT) is an acute phase protein that possesses immune-regulatory and anti-inflammatory functions independent of antiprotease activity. AAT deficiency (AATD) associated with early-onset emphysema chronic obstructive pulmonary disease. Of interest are the AATD nonsense mutations (termed null or Q0), majority which arise from premature termination codons in mRNA coding region. We have recently demonstrated plasma patient homozygous for Null Bolton allele (
AIMTo identify miRNA-regulated proteins differentially expressed between Caco2 and HT-29: two principal cell line models of the intestine. METHODSExponentially growing Caco-2 HT-29 cells were harvested prepared for mRNA, miRNA proteomic profiling.mRNA microarray profiling analysis was carried out using Affymetrix GeneChip Human Gene 1.0 ST array.miRNA Genechip 3.0 array.Quantitative Label-free LC-MS/MS performed a Dionex Ultimate 3000 RSLCnano system coupled to hybrid linear ion 7369
Abstract Scope Copper supplementation in nutrition has evolved from using inorganic mineral salts to organically chelated minerals but with limited knowledge of the impact at cellular level. Methods Here, and organic nutrient forms (glycinate, acid, proteinate) copper on level is investigated intestinal cell lines, HT 29 Caco‐2, after a 2‐hr acute exposure compounds following 10‐hr recovery. Results Following recovery, increases were observed proteins involved metal binding...